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Citations for New England Biolabs :
2151 - 2200 of 7433 citations for hsa mir 940 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... DNA was synthesized from extracted RNA using 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Immunology 2022Quote: ... The qPCR was performed using the NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with cycling conditions of 10 min at 55°C for reverse transcription ...
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bioRxiv - Genomics 2019Quote: ... The cell lysate was centrifuged for 5min at 2,000g at RT and suspended in 500μl of 1X Restriction buffer 2 (NEB). This step was repeated thrice ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 mM CaCl2 and the TRX-6His-S-tag was cleaved overnight at RT with enterokinase protease (NEB). The ET domain was further purified using Nickel-NTA resin (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... 1 mM ATP and 3 units of T4 DNA ligase for 2 h at RT (all from NEB). Next ...
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bioRxiv - Cancer Biology 2019Quote: ... ligation was carried out at RT for 2h using 2000 U of T4 DNA ligase (New England Biolabs). DH5α cells (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 ng RNA were used for analysis with the LUNA one-step RT-qPCR kit (LUNA E3005L Biolabs). The relative expression levels of the mRNA of interest were determined by real-time PCR using Quantifast SYBR Green Mix (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... Detection of selected targets was performed with Luna® Universal One-Step RT-qPCR (New England BioLabs Inc.) according to manufacturers protocol using specific primers ...
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bioRxiv - Neuroscience 2022Quote: ... Complementary DNA (cDNA) was synthesized from 200ng RNA using LunaScript® RT-SuperMix kit (New England Biolabs #E3010). RT-qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs #M3003) ...
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bioRxiv - Microbiology 2022Quote: ... viral cDNA was synthesized from the extracted RNA by using a LunarScript RT SuperMix Kit (New England BioLabs). The DNA was then amplified by performing a multiplexed PCR in two pools using the ARTIC-N5 primers and the Q5 Hot Start DNA polymerase (New England BioLabs ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized via reverse transcription from ~500 ng of RNA using LunaScript RT Supermix (New England Biolabs). Transcripts of interest were subsequently quantified using Luna qPCR master mix (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was prepared from 1µg of RNA using the LunaScript® RT SuperMix Kit (New England Biolabs, E3010L). A no template control (NTC ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR analysis was performed using New England Biotech LUNA SYBR Green qPCR reagents (New England Biolabs, U.K.). Glyceraldehyde phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2023Quote: RT-LAMP reactions were done using the WarmStart Colorimetric LAMP 2X Master Mix kit (M1800, NEB, Hitchin, UK) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was carried out with 1.5 mM (high concentration) or 0.1 mM (limiting concentration) dNTP mix and 2.5 U AMV-RT (NEB, M0277S) and 1 hour incubation at 42°C ...
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bioRxiv - Microbiology 2023Quote: ... viral cDNA was synthesized from the extracted RNA by using a LunarScript RT SuperMix Kit (New England BioLabs). The DNA was amplified by performing a multiplexed PCR in two pools using the ARTIC-N6 primers and the Q5 High-Fidelity DNA polymerase or Q5 Hot Start DNA polymerase (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... For ligation, samples were washed in CutSmart buffer, preincubated (5 min, RT) in 1x T4 ligase buffer (NEB), and incubated (18 h ...
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bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pif expression was assessed with the Luna® Universal Two-Step RT-qPCR Master Mix (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 500ng poly-A tailed RNA was ligated to ONT RT adaptor (RTA) using T4 DNA ligase (NEB, M0202M) and reverse transcribed by SuperScript III Reverse transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription (RT) of RNA was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs), in accordance with the manufacturer’s manual ...
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bioRxiv - Genetics 2021Quote: ... Tagmented library was amplified with P5_BRB and BRB_Idx7N5 primers (5 μL, Supplementary Table 14) using NEBNext UltraTM II Q5 Master Mix (NEB, M0544L) which was incubated at 98 °C for 30 sec before adding DNA with the following conditions ...
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bioRxiv - Genetics 2021Quote: ... 2nd cDNA strand was again synthesized using a transcript specific primer (either 5’UTR_βG-intron _F or 5’UTR_CMV _F2, Table 4) and Q5 2x master mix (NEB, #M0494). After another round of bead purification ...
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bioRxiv - Microbiology 2021Quote: ... pSRK vectors were constructed as previously described using primers listed in table S3 amplified using the polymerase Q5 (NEB). Soft Agar plates were prepared with 0.25% agar ...
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bioRxiv - Molecular Biology 2021Quote: The RNA primer was labelled at the 5’ terminus with [γ-32P]ATP using T4 PNK (New England Biolabs) and PAGE purified ...
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bioRxiv - Developmental Biology 2022Quote: ... and No Template Controls (NTC) for each primer pair using Luna Universal qCPR Master Mix (NEB, Ipswich, MA, #M3003) following the manufacturer’s protocol (1ul cDNA ...
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bioRxiv - Neuroscience 2019Quote: ... The NEBuilder Assembly tool was used to design primers for the NEBuilder HiFi DNA assembly (New England Biolabs # E2621S) of dNOS exon 16 as contained in pET28a-dNOS exon 16 (gift of Nikita Yakubovich ...
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bioRxiv - Microbiology 2020Quote: ... were carried out using specific primers (Supplemental Table 1) and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). After sequence verification ...
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bioRxiv - Developmental Biology 2019Quote: ... 200 ng of total RNA samples with RQN values >6.0 were used for cDNA synthesis together with the Oligo d(T)18 mRNA Primer (NEB) and SuperScript III Reverse Transcriptase (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... Barcode cassette was amplified from genomic DNA with primers P5.seq-B-GLI.v1 and P7.seq-B-GLI.v1 using OneTaq® DNA Polymerase (NEB, cat ...
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bioRxiv - Genetics 2021Quote: ... primers P1 and P2 (Table S1) were used with the Q5 Hot Start High-Fidelity 2X Master Mix (NEB). To ensure coverage for each sample ...
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bioRxiv - Genomics 2021Quote: ... cDNAs were generated using the ProtoScript II First Strand cDNA Synthesis Kit and Oligo(dT) primers (New England Biolabs). RT-qPCR were performed on the Light Cycler 480II (Roche ...
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bioRxiv - Systems Biology 2020Quote: ... The DNA samples incubated for primer extension as described previously13 were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Primer dimer artifacts were reduced by digesting the reverse-transcribed first-strand cDNA using lambda exonuclease (New England Biolabs) (37°C for 30 min ...
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bioRxiv - Plant Biology 2021Quote: ... We amplified the corresponding cDNAs using the primers on Table S1 and Q5® High-Fidelity DNA Polymerase (NEB). The PCR fragments were cloned into pENTR-D Topo vector and sequenced.
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bioRxiv - Plant Biology 2020Quote: ... The region of interest was amplified with specific primers (see Supplemental Table 4) and the Q5 DNA polymerase (NEB), and indexed by PCR using primers containing Illumina indexes (see Supplemental Table 4) ...
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bioRxiv - Cell Biology 2020Quote: ... Tagmentation reactions were completely used as input for a first PCR reaction with cassette-and Tn5-adapter-specific primers (5Btn-hmNeong.rv, P5.fw) with NEBNext Q5 HotStart polymerase (New England BioLabs) with 15 cycles of 68 °C and 1 min elongation ...
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bioRxiv - Cell Biology 2020Quote: ... These beads were then used as input of a second PCR with cassette-and Tn5-adapter-specific primers (P7-gri701…706-hmNeong.rv, P5.fw) with NEBNext Q5 HotStart polymerase (New England BioLabs) with 25 cycles of 68 °C and 1 min elongation ...
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bioRxiv - Microbiology 2021Quote: ... The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmented samples were amplified by the addition of 2.5μL each of 10μM barcoded forward and reverse primers (Picelli et al., 2014) and 15μL Q5 2x HiFI MasterMix (New England Biolabs) using the following thermocycling conditions ...
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bioRxiv - Cell Biology 2022Quote: ... PCR was performed using pBluescript SK+ CPES_V5 clone as template with appropriate sense and antisense primers (Table S1) and Phusion polymerase (NEB). The PCR product was digested with DpnI to remove templates followed by transformation into DH5α competent cells ...
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bioRxiv - Biochemistry 2022Quote: ... Mutants were created via side directed mutagenesis using primers designed on NEBaseChanger and Q5 site-directed mutagenesis kit following manufacturer’s directions (New England BioLabs). BL21(DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR was performed using pBluescript SK+ CPES_V5 clone as template with appropriate sense and antisense primers (Table S1) and Phusion polymerase (NEB). The PCR product was digested with DpnI to remove templates followed by transformation into DH5α competent cells ...
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bioRxiv - Biophysics 2022Quote: ... The two primers are annealed and the single strand overhangs are filled in using One-Taq DNA polymerase (NEB). These filled-in primers are then amplified by PCR with One-Taq DNA polymerase (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... each well containing 0.8 µL Primer Master Mix (0.225% Triton X-100, 1.6 mM dNTP mix, 1.875 uM barcoded oligo[dT] CEL-seq2 primers; Sigma-Aldrich, New England Biolabs) using a BioSorter (Union BioMetrica ...
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bioRxiv - Systems Biology 2019Quote: ... The DNA samples incubated for primer extension as described previously were treated with dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs ...
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bioRxiv - Genetics 2019Quote: ... 10 pmoles of forward and universal reverse primers were annealed and extended with Q5 high-fidelity DNA polymerase (NEB) according to the recommended conditions and with the following program ...
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bioRxiv - Neuroscience 2019Quote: Enhancers were cloned from C57Bl/6J genomic DNA using enhancer-specific primers and Phusion high-fidelity polymerase (M0530S; NEB). Individual enhancers were then inserted into an rAAV or scAAV backbone that contained a minimal beta-globin promoter ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and indexed with NEBNext® Multiplex Oligos for Illumina® (96 Index Primers) (New England Biolabs, Ipswich, Massachusetts, USA). Sample libraries at 1.1 pM were sequenced twice on the NextSeq500 ...