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Citations for New England Biolabs :
1951 - 2000 of 4230 citations for R 4 5 Isopropylidene 2 pentanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 mM dCTP and 2 U of terminal transferase (New England Biolabs). The mix was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated with 2 µM SNAP-Cell Oregon Green (NEB) for 20 minutes at 37°C to label newly synthesized RPL23a ...
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bioRxiv - Microbiology 2024Quote: ... The RNE-Apex2FlgC-EGFPC-2 plasmid was assembled via Gibson assembly (NEB) and transformed into chemically competent DHbeta10 E ...
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bioRxiv - Microbiology 2024Quote: ... 20 µl Q5® High-Fidelity DNA Polymerase (2 U/µl, NEB), 160 µl Taq DNA ligase (40 U/µl ...
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bioRxiv - Microbiology 2024Quote: ... cenocepacia K56-2 genome using Q5 polymerase with high GC buffer (NEB) and primers 3107 and 3108 (Supplementary Table 10 ...
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bioRxiv - Biochemistry 2024Quote: ... with or without 2 µL Lambda protein phosphatase (NEB, 400000 U/mL). Reactions were carried out for various times ...
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bioRxiv - Synthetic Biology 2024Quote: ... 2 to 3 μg plasmid was digested with 1 μl NotI (NEB) for 1 h at 37 °C and heat inactivated prior to transformation ...
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bioRxiv - Developmental Biology 2020Quote: ... 3mM MgCl2) for 5 min before digesting with 0.2U/ml of DNase I (NEB). Embryos were then fixed in 4% PFA ...
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bioRxiv - Developmental Biology 2021Quote: ... To each of those tubes 5 μl of 10x CutSmart Buffer (New England Biolabs) and 2 μl of DpnI restriction enzyme (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was resuspended and treated with 7.5 units (1.5 µl) RNA 5′ pyrophosphohydrolase (NEB) for 1 h at 37°C in a total volume of 20 µl ...
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bioRxiv - Molecular Biology 2021Quote: ... The bead slurry was directly treated with 3 µL Klenow (3’→5’ exo-) (NEB) in 50 µL NEB Buffer #2 with 0.2 mM ATP at 37 °C for 30 min to add 3’ overhangs to DNA ...
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bioRxiv - Cell Biology 2022Quote: ... and then unligated linker was digested away with 25 U Yeast 5’-deadenylase (NEB) and 5 U RecJ exonuclease (Epicentre) ...
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bioRxiv - Immunology 2020Quote: ... Samples (20 μL) were loaded in DNA loading buffer (5 μL; New England Biolabs) and gels were run at 60 V for 120 mins.
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bioRxiv - Molecular Biology 2020Quote: ... RNA was treated with RppH (RNA 5’ Pyrophosphohydrolase, Art. No. M0356S, New England Biolabs) to dephosphorylate small RNAs and specifically enrich for 22G RNAs ...
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bioRxiv - Genomics 2021Quote: ... 5 µL of 5X Phusion HF reaction buffer (all from New England Biolabs, USA), 0.5 µL of 10 mM premixed dNTPs and 0.5 µL of gDNA ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-gaattcgatgtgtaggctggag-3’ using Gibson assembly technique (kit was purchased from New England Biolabs), to yield pIJ773-Δspt9 ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: 5-10 μg RNA was diluted in 2x RNA loading dye (New England Biolabs) and size separated on 7 M Urea/15% polyacrylamide/0.5×TBE gels by gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
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bioRxiv - Biophysics 2020Quote: ... and 7U/mL Klenow Fragment of DNA Polymerase I (3’-5 exo-) (NEB, M0212). Reactions were incubated at 37°C and incorporation of labeled dCMP was monitored by an acid precipitation assay ...
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bioRxiv - Cell Biology 2022Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR (New England Biolabs) for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubating with either Klenow Fragment (3’→5’ exo-) (New England Biolabs (NEB), M0212L ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubating with either Klenow Fragment (3’→5’ exo-) (New England Biolabs (NEB), M0212L ...
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bioRxiv - Immunology 2020Quote: ... about 5 μg of the genomic DNA was digested with HaeIII (New England Biolabs) and purified using phenol:chloroform extraction followed by ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 2X Blunt/TA Ligase master mix (New England Biolabs, cat#M0367S), and milliQ water up to 10 μl total volume ...
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bioRxiv - Molecular Biology 2020Quote: ... which was treated with 5 units of T7E1 (New England Biolabs, Ipswich,MA, USA) for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the RNP injection mix [5 µM EnGen® Spy Cas9 NLS (NEB Cat# M0646T) mixed with 2.5 µM of each gRNA and Cas9 buffer] was freshly prepared ...
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bioRxiv - Zoology 2021Quote: ... we split the library and ran 5 high-fidelity Phusion (New England BioLabs, #M0530) PCRs for 12 cycles on a SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ adaptor ligation using T4 RNA Ligase 1 (New England Biolabs, Ipswich, MA, M0204L). Generation of cDNA was done by using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 ng of RNA was digested by 5 U of RppH (New England Biolabs) for 2 h at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... act57B sequence: 5’-UCUUCCCCUC-3’ RNA oligonucleotides were end-labeled using T4 Kinase (NEB) with ATP [γ-32P]32 ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
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bioRxiv - Genomics 2020Quote: ... incubated for 5 minutes and magnetically separated using a Magnetic Separation Rack (NEB #S1509S). Three 1 mL 70% ethanol washes are performed on the Eppendorf tubes ...
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Microbiology 2021Quote: ... dsRNA ladders and a 5’ Fluorescein-labeled RNA (300 nt) were provided by NEB and used as substrates for RNase I and RNase III activity assays ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 μM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Microbiology 2021Quote: ... 5’ RACE products were generated with Template Switching RT Enzyme Mix (New England Biolabs) using anchored poly(dT)23 and TSO (GCT AAT CAT TGC AAG CAG TGG TAT CAA CGC AGA GTA CAT rGrGrG ...
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bioRxiv - Molecular Biology 2021Quote: ... in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs). After dialysis ...
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bioRxiv - Neuroscience 2023Quote: ... containing 5 µl of collection buffer (NEBNext Single Cell Lysis Module, New England Biolabs). Noteworthy ...
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bioRxiv - Genomics 2023Quote: ... around 5 million cross-linked nuclei were digested overnight using 400 U DpnII (NEB). After digestion ...
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bioRxiv - Genomics 2023Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... 5 μl of genomic DNA was used with Luna Universal qPCR Master Mix (NEB) and primers for the R ...
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bioRxiv - Genomics 2023Quote: ... The DNA/RNA hybrid strand was pre-adenylated with DNA 5’ Adenylation Kit (NEB) and purified with Oligo Clean & Concentrator (Zymo) ...
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bioRxiv - Molecular Biology 2022Quote: ... The linkers that were pre-adenylated using a 5’ DNA adenylation mix (NEB, #E2610L) were ligated to dephosphorylated RNAs using T4 truncated RNA ligase 2 (K227Q ...
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bioRxiv - Genomics 2022Quote: ... the end-repaired DNA was mixed with Klenow Fragment (3′ → 5′ exo−) (NEB, #E6044A) in NEBNext dA-Tailing Reaction Buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNase-treated total RNA was further treated with RNA 5’ Pyrophosphohydrolase (New England Biolabs) to remove possible pyrophosphate from the 5’ end ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5′ end labelled using γ32P UTP (Perking Elmer) and T4 polynucleotide kinase (NEB) following manufacturer’s protocol ...