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Citations for New England Biolabs :
151 - 200 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... coli (NEB) as described above for StcE and sialidase ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (NEB) as described above for StcE and sialidase with the exception that expression was induced with IPTG at an OD600 of 0.9 The harvested cells were then lysed using sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB) ...
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bioRxiv - Genomics 2022Quote: ... ligated to Illumina Truseq LT adaptors using Quick Ligase enzyme (NEB) and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Synthetic Biology 2023Quote: ... The Python script by default selects codons with the highest usage frequency for the input organism (E. coli NEB 10-Beta in this study), unless specified problematic sequences are introduced (Supplementary Figure S3B) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli (NEB, #C2987H) by following the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: ... coli ligase (NEB). The longest product ...
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bioRxiv - Biophysics 2021Quote: ... coli cells (NEB) and was purified by maxiprep ...
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bioRxiv - Genetics 2021Quote: ... coli (C2987H, NEB), resultant colonies of which were screened for successful assembly with colony PCR —2 min initial denaturation (95 °C) ...
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bioRxiv - Microbiology 2021Quote: ... coli (NEB, #C3019I) were transformed with ligated DNA according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (NEB) and validated by sequencing using the universal primer U6-Forward.
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bioRxiv - Molecular Biology 2021Quote: ... coli DH5alpha (NEB) was used for all for cloning and sExpress 47 as a conjugal donor ...
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bioRxiv - Bioengineering 2019Quote: ... coli BL21 (NEB).
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bioRxiv - Synthetic Biology 2019Quote: ... coli strain (NEB) using ZymoPURE Midi Kit (Zymo Research) ...
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bioRxiv - Bioengineering 2019Quote: ... coli cells (NEB)
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bioRxiv - Cancer Biology 2020Quote: ... coli (NEB #3020K), and the entire transformation was grown as a midi culture and prepped with Chargeswitch Pro Filter Midi Kit (Thermofisher #CS31104).
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bioRxiv - Cancer Biology 2020Quote: ... coli (NEB #3040H) and cells were grown at 30 °C for 24 h ...
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bioRxiv - Immunology 2021Quote: ... coli cells (NEB), and the isolated plasmids were verified through sanger sequencing.
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bioRxiv - Genomics 2021Quote: ... coli (NEB #C2566). Cells were cultured in LB media until an OD600 of 0.6 and induced with 0.4 mM IPTG (Growcells #MESP-2002 ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli Turbo (NEB) was used throughout this study ...
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bioRxiv - Microbiology 2021Quote: ... coli cells (NEB). All constructs were N-terminally truncated to remove the transmembrane helix (α1N ...
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bioRxiv - Biochemistry 2020Quote: ... coli ER2738 (NEB) for 15 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... coli cells (NEB). Constructs were validated by Sanger sequencing.
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bioRxiv - Bioengineering 2021Quote: ... coli cells (NEB).
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bioRxiv - Bioengineering 2021Quote: ... coli cell (NEB). Cells were grown at 37°C to log phase to achieve OD 0.8 ...
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bioRxiv - Bioengineering 2021Quote: ... coli DH5a (NEB) was used for general plasmid propagation and standard molecular techniques ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell (NEB) for expression ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell (NEB) for expression ...
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bioRxiv - Biochemistry 2021Quote: ... coli cell (NEB) as follows ...
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bioRxiv - Biochemistry 2020Quote: ... coli (NEB, UK). Protein expression was carried out in MagicMedia™ E ...
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bioRxiv - Biochemistry 2020Quote: ... coli (NEB, UK). Protein expression was carried out in MagicMedia™ E ...
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bioRxiv - Cancer Biology 2020Quote: ... coli (NEB#C2987).
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB-c3026) was transformed with pET-28a(+)-OsRGLP1 and grown in 100 mL LB media with 100 mg/L kanamycin at 30 °C to an OD600nm 0.6 ...
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bioRxiv - Systems Biology 2021Quote: ... coli (NEB C2987I) were transformed with pDONR221 containing the Akt KTR ...
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bioRxiv - Microbiology 2020Quote: ... coli DH5α (NEB) according to the manufacturer’s instructions
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bioRxiv - Microbiology 2021Quote: ... coli (NEB 5alpha). The resulting constructs were confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... coli (10β, NEB) for plasmid propagation ...
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bioRxiv - Microbiology 2021Quote: ... coli strain (NEB) and protein expression was done following the manufacturer directions with some modifications ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cells (NEB) to generate the final barcoded piggyBac pool (final identifier P58) ...
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bioRxiv - Cell Biology 2021Quote: ... coli (NEB C2527) using isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible expression systems and amino-terminal hexa histidine tags ...
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bioRxiv - Genomics 2020Quote: ... coli (NEB #C3019H) according to manufacturer protocol ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (NEB) and expressed as GST-fusion proteins ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB C3020K) and plated in duplicate at a target bottleneck of 50,000 unique barcodes per library (100,000 for Delta libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells (NEB). Recombinant clones were sequenced using the universal primers M13-Forward and M13-Reverse.
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bioRxiv - Microbiology 2022Quote: ... Coli (NEB C3040H). Purified EMCV-IRES-PV plasmid DNA was linearized using EcoRI digestion and purified linear DNA was then used to in vitro transcribe EMCV-IRES-PV plus stranded RNA using MEGAscript™ T7 Transcription Kit according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... coli (NEB, C2987H) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (NEB) and expressed as a His-tagged protein in LB media supplemented with 60 µg/mL of kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... coli Turbo (NEB) and DNA was recovered with Macherey-Nagel NucleoBond Xtra Midi kit.
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells (NEB), from which demethylated pSG01 was obtained for all downstream applications to increase the efficiency of B ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli (NEB, #C3019) and selected on LB agar plates (ThermoFisher ...