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Citations for New England Biolabs :
51 - 100 of 2029 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... was performed using 10ng of His tagged PTP-PEST (WT) plasmid as template and followed by Dpn1 (New England Biolabs, UK) digestion for 2 hours at 37°C ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Molecular Biology 2021Quote: ... The transcription template for RNAI was produced by using p770 plasmid (from E. coli strain LK_2385) as template for PCR amplification using Q5 polymerase (NEB) and LK_3413 and LK_3414 primers to introduce a T7 class II promoter (Supplementary table 3) ...
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bioRxiv - Molecular Biology 2022Quote: The recombinant pET6xHN-N constructs were transformed into Escherichia coli strain BL21(DE3) (New England Biolabs, MA, USA). The transformed clones were cultured at 37 °C in LB medium with 100 μg/mL Carbenicillin and were induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Molecular Biology 2019Quote: ... The HIS-SUMO-BAHEPR-1 fusion and HIS-SUMO tag constructs were expressed in Escherichia coli BL21 DE3 cells (New England BioLabs). Both HIS-SUMO-BAHEPR-1 and HIS-SUMO cultures were initially grown in 4 liters of LB media at 37 °C at 200 RPM until cultures reached an optical density of ∼ 1.0 at 600 nm and then cultures were pelleted ...
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bioRxiv - Biophysics 2022Quote: ... a construct was cloned by PCR from the LacZ gene of Escherichia coli MG1655 with a His-tag on the C terminus by Gibson Assembly (New England Biolabs) in a pET-28b vector using the following primers LacZ200 (TAACTTTAAG AAGGAGATAT ACCATGACCA TGATTACGGA TTCACTGGCC GTCGTTTTAC ...
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bioRxiv - Developmental Biology 2021Quote: ... They were then incubated overnight at 37 °C in Hybridization Buffer (10% Formamide, 10% 20x SSC, 400 µg/ml E. coli tRNA (New England Biolabs), 5% dextran sulfate ...
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bioRxiv - Microbiology 2021Quote: ... Capped mRNAs were again purified by LiCl4 precipitation followed by the addition of polyA tails using E coli Poly(A) polymerase (NEB) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The recombinant constructs (pETDuet-1-TaPHB-1 and pETDuet-1-TaPHB-1-RUVBL1 were separately transformed into E. coli Lemo21(DE3) (NEB) chemical competent cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Immunology 2023Quote: ... Unincorporated primers were digested from the pre-amplified samples using 16 U/μl Exonuclease I (E. coli, New England Biolabs) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... gap fill enzymes were added (1U Sulfolobus DNA Polymerase IV NEB cat# M0327S and 10U E. coli DNA Ligase NEB cat# M0205L) and incubation continued for an additional 30min at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Plant Biology 2023Quote: ... MBP-tagged and GST-tagged proteins were detected using anti-MBP (E8032S, NEB) and anti-GST antibody (60-021 ...
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bioRxiv - Immunology 2021Quote: ... coli (NEB) accomplished by heat shock at 42°C ...
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bioRxiv - Developmental Biology 2021Quote: ... coli (BioLabs) strains ...
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bioRxiv - Developmental Biology 2020Quote: ... coli (NEB).
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bioRxiv - Biochemistry 2022Quote: ... coli (NEB) were transformed ...
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bioRxiv - Genetics 2019Quote: ... coli (NEB), by electroporation in a Bio-Rad GenePulser machine (2.0k V ...
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB) using the heat shock protocol provided by the manufacturer and the transformants grown on LB agar plates supplemented with ampicillin (100 µg/mL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli (NEB) as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli (NEB) by adding 10 μL to each ...
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) and purified using the PureYield MidiPrep system (Promega).
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) was suspended in 3 mL of LB and grown overnight ...
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) was scaled up to aid coverage ...
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bioRxiv - Cell Biology 2021Quote: ... coli (NEB). The reporter construct for ASAP2f was assembled into 2 different Level 1 destination vectors ...
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB) were chemically transformed ...
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bioRxiv - Biochemistry 2021Quote: ... coli (NEB).
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bioRxiv - Microbiology 2021Quote: ... coli (NEB) were transformed with 3 μl of the Gibson mix and plated on four LB plates containing ampicillin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... coli (NEB 10-beta electrocompetent cells ...
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bioRxiv - Biochemistry 2020Quote: ... coli (NEB) were co-transformed with 1 ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... coli (NEB) and purified as described in Yeeles et al ...
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bioRxiv - Bioengineering 2022Quote: ... coli (NEB).
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bioRxiv - Bioengineering 2022Quote: ... coli (NEB). Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... coli (NEB) after adding 0.25 mM IPTG for 4 hours at 28°C ...
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bioRxiv - Cancer Biology 2022Quote: ... coli (NEB).
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bioRxiv - Molecular Biology 2019Quote: ... coli (NEB) were transformed and inoculated in LB media with kanamycin and grown to an A600 of 0.6 ...
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bioRxiv - Bioengineering 2021Quote: ... coli (NEB) were used for the expression of DsbC-his ...
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bioRxiv - Genetics 2022Quote: ... coli (NEB). The hsp68 promoter driving dsRed reporter was cloned using EcoRI and BamHI sites ...
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bioRxiv - Biophysics 2019Quote: ... coli (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... coli (NEB) and production was induced by 0.1M IPTG treatment ...
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bioRxiv - Bioengineering 2020Quote: ... coli (NEB) by chemical transformation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli (NEB) and purified following standard Ni-NTA resin purification protocols (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... coli (NEB). All cultures were grown in 2×YT media ...
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bioRxiv - Cancer Biology 2020Quote: ... coli (NEB) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... coli (NEB) to generate NDV_LS_S ...
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bioRxiv - Microbiology 2021Quote: ... coli (NEB) and selected on LB agar (per liter ...
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bioRxiv - Immunology 2022Quote: ... coli (NEB 10-beta electrocompetent cells ...
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bioRxiv - Microbiology 2022Quote: ... coli (NEB), and isolated plasmid was verified by restriction enzyme digest and sequencing by Eurofins Genomics ...
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bioRxiv - Bioengineering 2022Quote: ... coli (NEB). Fifty μL of electrocompetent E ...