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Citations for New England Biolabs :
151 - 189 of 189 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... after removing the Gel Coverslip, slides were incubated in 5 ml warmed Clearing Solution (ref. 20300003, Vizgen) with Proteinase K (ref. P8107S, NEB) in a sealed petri dish for 24 hours at 37°C.
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bioRxiv - Genetics 2024Quote: ... The cell suspension was subsequently treated with an equal amount of Solution B containing 100 IU/mL of DNase I (New England Biolabs), 0.5% BSA (Sigma Aldrich) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we ligated 100 ng of sample DNA in 15 µL of a solution with 1X CutSmart Buffer (New England Biolabs, USA; NEB), 10 units of XbaI ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin and RNA were digested in 100 µL MNase digestion solution (1x micrococcal nuclease (MNase) buffer and 40 units/µl MNase (NEB, #M0247)) for 5 min at 37 °C while shaking at 1,400 rpm in a thermomixer ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL of 10 mM indexed P5 and P7 primer solutions and 25 µL NEBnext High-Fidelity 2X Master Mix (New England BioLabs: ME541L) were added ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of the heated solution was then used as template for PCR using the Q5® High-Fidelity DNA Polymerase (NEB), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were cleared for 24-48 h with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μg of venom in 10 µL of reducing SDS-PAGE buffer (6X stock solution, NEB B7024S with 30% β-mercaptoethanol) was run per lane on BioRad™ Mini PROTEAN pre-cast TGX acrylamide gels (15 well ...
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bioRxiv - Neuroscience 2023Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS + 2% BSA + 0.2U/µl RNase inhibitor - New England Biolabs, Cat#: M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their shape ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-5 μM SNAPf fused activator was incubated in a 1× PBS solution containing up to threefold excess of SNAP-surface 649 (New England BioLabs, #S9159S), 1 mM DTT ...
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bioRxiv - Developmental Biology 2023Quote: One-cell stage zebrafish embryos were injected with 1-2 nl of injection solution containing 300 ng/μl of Cas9 enzyme (NEB# M0646T) and 12.5 ng//μl of sgRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... the tissue samples were stained using Vizgen’s Cell Boundary Kit (Vizgen, 10400009) and blocked in blocking solution (Vizgen, 20300012) supplemented with a 1:20 dilution of Rnase inhibitor (NEB, M0314L) for one hour ...
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bioRxiv - Molecular Biology 2024Quote: ... This incubation was repeated with a 1:200 pA-Dam solution (equivalent to nearly 60 Dam units, determined by calibration against Dam enzyme from NEB, #M0222L), followed by two wash steps ...
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bioRxiv - Neuroscience 2024Quote: ... Final nuclear lysates were resuspended using 100 µl of resuspension solution (1x PBS, 2% BSA, 0.2U/µl RNase inhibitor; New England Biolabs, cat# M0314S). Hoechst staining was performed to assess the quality of isolated nuclei based on their morphology ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 h at 37°C and then cleared for 16 to 24 hours with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the Manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... sections were cleared for 24-48 hours with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 5 uL of gDNA solution was used as template in the 50 uL PCR reaction using Q5 High-Fidelity 2X Master Mix (NEB, M0492) with the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... This incubation was repeated with a 1:200 pA-Dam solution (equivalent to nearly 60 Dam units, determined by calibration against Dam enzyme from NEB, #M0222L), followed by two wash steps ...
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bioRxiv - Bioengineering 2024Quote: ... The WarmStart Master Mix is designated because the enzyme is inactive at lower temperatures but becomes active once the reaction solution reaches a temperature of 40°C (NEB, USA). Using the enzyme and indicator of this recent invention ...
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bioRxiv - Biophysics 2024Quote: ... dynactin and NINL was measured in solution by coupling 100nM NINL to 35 µL of NEBExpress® Ni-NTA Magnetic Beads (NEB) in the case of NINL-dynactin binding or 100nM dynein to 25 µL SNAP-Capture Magnetic Beads (NEB ...
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bioRxiv - Genomics 2020Quote: ... we heated samples to 95°C for 3min and snap-cooled on an ice slush before gently adding 0.5μl of enzyme solution (8,000 U/ml Bst DNA Polymerase Large Fragment, New England Biolabs, USA, in 1X amplification buffer) into the aqueous droplet.
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bioRxiv - Genomics 2022Quote: ... Chimeric RNA barcode molecules were eluted from the bead by incubating with 127 µL ProK digestion solution (11 µL ProK (NEB cat # P8107B),100 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... to 10 μl of the plasma/stop solution 70 μl of mixture consisting of 75 mU/ml ATP sulfurylase (New England Biolabs, Cambridge, MA), 1 μmol/L APS (Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Bioengineering 2022Quote: Reunion of 4-6 nM of dsDNA template (gblocks® Gene Fragment, IDT) to a solution of 5 U/μL of T7 RNA polymerase (NEB, M0251), 200 nM Cas9 (S ...
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bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... halys adults were pooled in 400 µL of a DNA/RNA-protecting solution (Monarch Total RNA Miniprep Kit, New England Biolabs Inc., Germany) for each biological replicate ...
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bioRxiv - Cell Biology 2024Quote: In vitro kinase reaction was performed in 40 μl solution consisting of 1X NEBuffer™ for Protein Kinases (PK) (New England Biolabs, B6022SVIAL), 2 mM ATP-gamma-S Kinase substrate (Abcam ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we ligated 100 ng of sample DNA in 15 µL of a solution with 1X CutSmart Buffer (New England Biolabs, USA; NEB), 10 units of XbaI ...
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bioRxiv - Cancer Biology 2021Quote: ... Purification of PCR products was performed with an in-house ExoSAP solution [50 μl of Exonuclease I (20,000 units/ml) (NEB® M0568, Ipswich, MA); 40 μl of Antarctic Phosphatase (5,000 units/ml) ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of 5 μl of UDG reaction solution (New England Biolabs, 0.02 U μl−1 UDG in 1X UDG buffer) and an additional 30 min incubation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: Living ovaries were incubated at room temperature in SNAP solution containing 3 μM SNAP- Cell TMR-Star or SNAP-Cell 647SiR (New England BioLabs, S9105S and S9102S), 10% FCS and 0,2 mg/ml Insulin in Schneider’s medium ...
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bioRxiv - Genomics 2023Quote: ... This 17 µl of DNA solution was incubated with 3 µl digestion mixture containing 1 µl BciVI (New England Biolabs, cat. no. R0596S) and 2 µl CutSmart buffer (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... cells were incubated for 2 hours in a dye solution containing 1 µM SNAP-tag ligand BG-AF647 (New England Biolabs, catalog no. S9136S), 1 mM dithiothreitol (neoFroxx ...
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bioRxiv - Biochemistry 2024Quote: ... The DNA oligonucleotides in the transcribed RNAs solution were digested using DNase I (RNase-free) (Catalog no. M0303L, New England Biolabs Pte. Ltd., Singapore). RNAs were cleaned and eluted using Monarch® RNA Cleanup Kit (Catalog no ...
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bioRxiv - Genomics 2024Quote: ... The final rRNA depletion probe pool was made by combining the NEBNext rRNA Depletion Solution from NEBNext rRNA Depletion Kit V2 (Human/Mouse/Rat; New England Biolabs; Cat. No. E7405) and NEBNext rRNA Depletion Kit V2 (Bacteria ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...