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Citations for New England Biolabs :
51 - 100 of 189 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting aqueous solution was concentrated using a spin column (Monarch, NEB) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was assembled with the following: 3.2 µL solution A (NEB), 1 µL factor mix (NEB) ...
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bioRxiv - Biochemistry 2022Quote: ... The working solution at 1.53 μM was obtained using Diluent A (NEB) and 1% Triton X-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by in-solution DNase I treatment (8 Units, New England Biolabs) according to manufacturer’s instructions for 30 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.5 mg DNase I solution (New England Biolabs, Ipswich MA, USA) in PBS and incubated at 37 °C and 5 % CO2 for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, N0446), 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S) ...
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bioRxiv - Bioengineering 2021Quote: ... the plasmid solutions were incubated for Nb.Bsmi nicking enzyme (R0706S, New England Biolabs) for 60 minutes at 65°C in NEBuffer 3.1 ...
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bioRxiv - Genomics 2020Quote: ... 2 μl 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, #N0446), 10 μl 10x T4 DNA Ligase Reaction Buffer (NEB #B0202S) ...
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bioRxiv - Biophysics 2021Quote: ... 200μl of a 50ng/μl solution (using Lambda DNA, New England Biolabs (NEB) N3011S) ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched on ice and by addition of 1x Stop Solution (NEB). Immediate isopropanol-based precipitation recovered 75- 95% of input mRNA as mRNA fragments in water.
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bioRxiv - Neuroscience 2022Quote: RNA solution was then treated with DNase I (RNase-free) (New England Biolabs) to remove genomic DNA from sample and protected against RNA degradation using RNasin® Ribonuclease Inhibitor (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 μl of deoxynucleotide (dNTP) solution mix (40 μM; New England Biolabs, UK), 1 μl of forward primer (10 μM ...
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bioRxiv - Biophysics 2023Quote: ... The solution was supplemented with 0.1 vol of 10X T4 ligase buffer (NEB) and T4 ligase (30 U.μL-1 ...
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bioRxiv - Genomics 2024Quote: ... a 750 μl solution containing 1x NEB T4 DNA ligase buffer (NEB B0202), 120 μg of BSA (ThermoFisher AM2616) ...
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bioRxiv - Microbiology 2024Quote: ... the chamber was blocked with 1 mg/ml bovine serum albumin solution (NEB) for 5 min ...
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bioRxiv - Microbiology 2024Quote: ... the chamber was blocked with 1 mg/ml bovine serum albumin solution (NEB) for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The complexation reaction was carried out in a solution containing 1X NEBuffer 2.1 (NEB) (composed of 100 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... This was repeated with a 1:200 pA-Dam solution (∼60 NEB Dam units), followed by 2 wash steps ...
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bioRxiv - Synthetic Biology 2021Quote: ... These reaction solutions included the same reagents as the commercial reaction buffer from NEB but without Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: ... First, 200μl of a 50ng/μl solution (using Lambda DNA, New England Biolabs (NEB) N3011S) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 1 μL deoxynucleotide Solution Mix and 0.5 μL Taq DNA Polymerase (New England Biolabs). The reactions were incubated in an Eppendorf Mastercycler Gradient Thermal Cycler for 7m at 95°C ...
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bioRxiv - Cell Biology 2024Quote: ... and ligated with the oligo solution according to the NEB Quick Ligase (NEB, M2200) protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then immediately added 5 µL of a solution with 1X Ligase Buffer (NEB), 0.75 mM ATP (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... mRNAs were isolated from this solution using 50 μ magnetic Oligo(dT)25 beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: A working lysis buffer solution was prepared by adding 1 µL of the MNase (NEB) [1:50 dilution] per 5 µL lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The solution was diluted to 200 μL to yield 1X NEBuffer 1 (NEB, Ipswich, MA) at pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: An in vitro translation reaction was assembled with the following: 6.4 µL solution A (NEB), 2 µL factor mix (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... Half of the purified PVS10-DLK solution was nicked with Nt.BbvCI enzyme (New England Biolabs) over 72 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were cleared with Clearing Solution consisting of 50μL of Proteinase K (NEB, P8107S) and 5mL of Clearing Premix (Vizgen 20300003 ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μg of gp145 in solution were incubated with 50 units of α2-3,6,8,9 NEUA (NEB) for 24 hrs at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... PV stock solution (1 mM) was prepared by adding 10 μl of 100 mM vanadate (NEB) and 50 μl of 100 mM hydrogen peroxide (diluted from a 30 % stock in 20 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Microbiology 2023Quote: ... to 100 μl of RNA solution and cleaned up with a Monarch RNA cleanup kit (NEB). RNA concentrations were measured with a NanoDrop 1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µL PCR Anchor Primer,1 µL Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, Inc., N0447L), 10 µL Q5® Reaction Buffer (New England BioLabs ...
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bioRxiv - Biophysics 2022Quote: ... cells were incubated in dye solution (1 μM BG-AF647 (catalog no. S9136S, New England Biolabs), 1 mM DTT (catalog no ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µL of the solution was mixed with 15 µL of amylose beads (New England Biolabs). Samples were placed into PierceTM micro-spin columns (Thermofischer ...
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bioRxiv - Microbiology 2022Quote: ... each chamber is filled for 30 min with a 1 mg/ml solution of Streptavidin (NEB) and then passivated with a blocking solution (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL NEBNext Bacterial rRNA depletion solution and 2 µL Probe Hybridization Buffer (NEB, CN E7850) were mixed with cells on ice ...
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bioRxiv - Plant Biology 2023Quote: ... The solution was then used to transform NEB turbo Escherichia coli competent cells (NEB, MA, USA). pCambia1300/SPRI1A-C+12 was created in our previous study (Fujii et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Transposed DNA was amplified in a solution containing 1X NEBNextHigh Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer ...
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bioRxiv - Neuroscience 2024Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Genomics 2024Quote: ... Spat tissue was then placed into a digestive solution containing lysis buffer and proteinase K (NEB). Samples were homogenized using a sterile pestle and digested at 56 °C for 2 hours ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were subjected to a methylation labeling reaction using a solution of 1x M.CviPI Reaction Buffer (NEB), 300 mM sucrose ...
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bioRxiv - Microbiology 2020Quote: ... Stock solutions of SNAP-Cell® TMR-Star or SNAP-Cell® 647-SiR (New England Biolabs) in DMSO were diluted to 4 μM in complete medium ...
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bioRxiv - Genetics 2020Quote: ... 10 mL of a 10% BSA solution was incubated with 2.5 μL of Proteinase K (NEB P8107S) for 30 minutes at room temperature (∼22°C) ...
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bioRxiv - Biochemistry 2023Quote: ... 50 ul of aqueous solution were recovered and treated with 0.3 U of calf intestinal phosphatase (NEB) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... beads were washed twice with coIP-solution and finally dissolved in RNA protection buffer (New England Biolabs).
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bioRxiv - Cancer Biology 2023Quote: ... and the samples were incubated overnight at 47℃ in clearing solution comprised of Protease K (NEB, P8107S) and Clearing Premix (Vizgen ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting solution was cooled down to 42°C before adding 2 U of β-agarase (NEB), gently mixing the solution by end-over-tube inversion ...
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bioRxiv - Biophysics 2024Quote: ... solutions containing polySH3 and cleaved MBP- and His6-tags were passed over Amylose resin (New England Biolabs) to remove excess MBP ...