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Citations for New England Biolabs :
151 - 200 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified using NEBMonarch PCR and DNA Clean-up Kit (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using the Monarch® PCR & DNA clean-up kit (New England Biolabs). In vitro expression was performed for 2 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR products were purified using the Monarch® PCR & DNA Cleanup Kit (NEB, T1030) and used as “megaprimers” that are denatured and annealed to the original plasmid (pNG93 ...
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bioRxiv - Biochemistry 2023Quote: ... RT-PCR reactions were performed using the OneTaq One-Step RT-PCR Kit (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Molecular Biology 2023Quote: 150 ng of genomic DNA extracted from ear punch biopsies (see Genotyping protocol) was digested with MseI (NEB) and 1x CutSmart buffer at 37°C for two hours ...
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bioRxiv - Microbiology 2019Quote: KLRC2 genotypes were determined by touchdown PCR using the Phusion High Fidelity PCR kit (New England Biolabs) using previously described methods40 ...
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... PCR was performed according to manufacturer’s protocol (Phusion High-Fidelity PCR kit, New England Biolabs, Ipswich, MA).
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bioRxiv - Genetics 2021Quote: ... The A-tailed PCR products were cleaned with Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB) and ligated into pGEM®-T Easy vector (Promega ...
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bioRxiv - Genetics 2020Quote: ... DNA libraries were then prepared by PCR amplification using NEBNext High-Fidelity PCR kit (New England Biolabs) in the presence of barcoded PCR primers (sequences provided in Ref ...
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bioRxiv - Synthetic Biology 2019Quote: ... PCR products with the expected size were column-purified with the Monarch PCR & DNA Cleanup Kit (NEB) and Sanger sequenced by GATC-Eurofins or Microsynth ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was performed using the Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, UK) and the final reaction contained 250 ng of template DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Eluted DNA was enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was cleaned using the Monarch® PCR clean up kit (New England Biolabs T1030S) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... The PCR products were purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs, no. T1030L). In vitro transcription of pegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and Luna® Universal RT-PCR Kit (New England Biolabs) in a StepOnePlus apparatus (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... NEB One Taq One-Step RT-PCR Kit (NEB # E5310S) was used for nucleic acid amplification ...
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bioRxiv - Developmental Biology 2019Quote: ... purified with the Monarch PCR and DNA Cleanup kit (NEB), and then dialyzed with 0.5X TE buffer using a 0.025 μm VSWP membrane (Millipore ...
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bioRxiv - Genomics 2021Quote: ... After purification with Monarch PCR & DNA Cleanup kit (NEB, #T1030S), libraries were indexed using NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was purified using a Monarch PCR purification kit (NEB). qPCR for three independent replicates was performed using iQ SYBR Green Supermix (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). Collected DNA was quantified with the Qubit dsDNA High Sensitivity Assay kit (ThermoFisher) ...
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bioRxiv - Synthetic Biology 2019Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). Note that a complete digestion of receiver vectors in both step 1 and 2 is key to avoid the recovery of empty backbones ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA was purified with Monarch PCR & DNA Cleanup Kit (NEB) and eluted in 20 µL of nuclease-free water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cleaned with the Monarch PCR & DNA cleanup kit (NEB). Pooled libraries were sent to the Beijing Genomics Institute (BGI ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified with the Monarch PCR & DNA Cleanup Kit (NEB). The pLentiGuide-Blast plasmid was digested with BsmBI-V2 (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... They were introduced by mutagenic PCR (Q5 mutagenesis kit, NEB) of the BtubAB expressing plasmid (Schlieper et al ...
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bioRxiv - Synthetic Biology 2022Quote: ... After purification using Monarch® PCR & DNA Cleanup kit (NEB), the templates were transcribed ...
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bioRxiv - Plant Biology 2023Quote: ... and purified using Monarch PCR & DNA Cleanup kit (NEB #T1030). The proUBE2:mTurquoise-N7 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products were purified with a DNA purification kit (NEB) and were then phosphorylated using T4 polynucleotide kinase (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... or Monarch PCR and DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2023Quote: ... We used the Monarch PCR and DNA Cleanup Kit (NEB) to purify PCR products ...
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bioRxiv - Developmental Biology 2024Quote: The PCR-based Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) was used to perform truncation (constructs L-P ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were pooled and purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Sequenced PCR products showing overlapping peaks in their chromatograms were cloned using NEB PCR cloning kit (NEB #E1202S) and then sequenced to define the identity of mutations carried by F1 heterozygous animals ...
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bioRxiv - Microbiology 2019Quote: ... the PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg; New England Biolabs GmbH). For the second PCR ...
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bioRxiv - Biophysics 2022Quote: ... PCR cleanup was carried out on each amplicon sample using the Monarch PCR cleanup kit (New England Biolabs). The amplicons were then dual-indexed for paired-end read Illumina sequencing using Nextera primers N701 and N702 for the PEL and SUP of the 5.5 h time point and N705 and N706 for the PEL and SUP of the 9.0 h time point ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
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bioRxiv - Molecular Biology 2023Quote: The amplicons from the Q5 PCR step were cleaned up using Monarch® PCR & DNA Cleanup Kit (NEB). The purity and size of the DNA bands were visualized by running a 1% agarose gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Zoology 2024Quote: ... PCR products were purified with a Monarch PCR & DNA clean-up kit (New England Biolabs, Whitby, ON, Canada) and used as template to reamplify the products to incorporate a Kozak translation initiation sequence prior to the start codon (Kozak ...
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bioRxiv - Molecular Biology 2023Quote: ... three 25 μl PCR reactions were pooled and purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB), according to manufacturer’s instructions ...