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Citations for New England Biolabs :
151 - 200 of 2119 citations for Human IgG1 Anti SARS CoV 2 Spike S1 Antibody CR3022 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: Expression vectors (SCRIPT 1-4; Supplemental Figure S1) were constructed by a Golden Gate reaction with BsaI (New England Biolabs) using the paired gRNA entry vectors and a destination vector as previously described (Decaestecker et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... the anti-Smt3 shRNA sequence based on the TRiP line HMS01540 (Supplementary Table S1) was ligated into the pValium20 vector (Ni et al. 2011) using T4 DNA ligase from NEB, according to the manufacturer’s instructions and was integrated at the attP2 landing site by BestGene ...
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bioRxiv - Biochemistry 2019Quote: ... 4 μg of the gapped plasmid was annealed to the appropriate 39 nt oligonucleotides (1:100 molar ratio, Supplementary Table S1) and ligated overnight with T4 DNA Ligase (NEB). Restoration of a KpnI restriction site present in the gapped region confirmed incorporation of mismatch oligonucleotides ...
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bioRxiv - Cell Biology 2020Quote: ... PCR amplification was performed with primers targeting the indicated regions (Fig. 1B, Supplemental Table S1) using Taq DNA Polymerase (New England Biolabs) for 30 cycles of amplifying protocol ...
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bioRxiv - Biophysics 2021Quote: ... the corresponding set of specific primers (Table S1) and digestion of the template from the reaction mix with DpnI (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RNA was ligated overnight at 16°C at 5’ with DNA/RNA chimeric oligonucleotide adaptor (TCAGACGTGTGCTCTTCCGATCTrNrNrWrNrNrWrNrN, TIF2-RNA in Supplementary Table S1 using T4 RNA ligase (NEB) in the presence of 10% dimethylsulphoxide (DMSO) ...
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bioRxiv - Genetics 2020Quote: ... An 18-mer poly-N barcode was created by annealing primers (Table S1) and extended to make fully double stranded with Klenow polymerase (NEB). The barcode was then PCR purified (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: ... by PCR based site-directed mutagenesis (SDM) using non-overlapping primers listed in Supplementary Table S1 and Phusion High-Fidelity DNA Polymerase (New England Biolabs). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: Primers (Table S1) were ordered from Integrated DNA Technologies (IDT) and constructs were PCR-amplified with Q5 High Fidelity polymerase (NEB). For all vectors ...
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bioRxiv - Molecular Biology 2023Quote: ... For library generation primers specific to the 5′ and 3′ RNA adapter sequence were synthesized (Table S1) and the whole cDNA was PCR amplified using the NEB Q5 HotStart polymerase (NEB). Secondary PCR was performed to introduce TrueSeq barcodes [16] ...
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bioRxiv - Cell Biology 2023Quote: The new constructs produced in this study are reported in S1 Table and were generated either through restriction enzymes mediated cloning or NEBuilder HiFi DNA Assembly (NEB) using the primers listed in S2 Table ...
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bioRxiv - Microbiology 2023Quote: ... RH-Luc+ Δgra47 or ME49-cLuc+ Δgra47 were co-transfected with plasmids containing sgRNAs specifically targeting the UPRT locus (Table S1) and EcoRV (New England Biolabs)-linearized pUPRT::GRA47HA plasmid at a ratio 1:5 of sgRNAs to linearized plasmid ...
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bioRxiv - Microbiology 2023Quote: ... we co-transfected the pU6-Universal plasmid carrying a sgRNA targeting the gene of interest (Table S1) and the BamHI-HF (New England Biolabs) linearized pTKOatt plasmid ...
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Broad variation in response of individual introns to splicing inhibitors in a humanized yeast strainbioRxiv - Molecular Biology 2023Quote: ... This plasmid was cut with BaeI and a guide sequence DNA targeting the genomic region encoding Hsh155 HRs 15-16 region (Table S1) was inserted using HiFi DNA Assembly (New England BioLabs) to make p416-TEF1p-Cas9-NLS-crRNA-HSH155.
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bioRxiv - Cell Biology 2023Quote: ... Bisulfite-converted DNA was amplified using the oligonucleotides #2479 and #2480 (Supplemental Table S1) and the Q5U Hot Start High-Fidelity DNA Polymerase (New England Biolabs). PCR products were sequenced at Eurofins Scientific using the NGSelect Amplicon option (2-step amplicon generation workflow).
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bioRxiv - Genetics 2023Quote: Libraries for 12 samples (Table S1) were prepared using the NEBNext Enzymatic Methyl-seq Kit (New England Biolabs, Massachusetts, USA) following the manufacturer’s large insert libraries protocol ...
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bioRxiv - Biochemistry 2024Quote: ... were PCR amplified with indicated primers (see Table S1) and inserted into backbone plasmids via restriction digest and ligation (all enzymes from NEB) or Gateway cloning (Thermo) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sox17 and sox32 deletion and domain switch constructs (Table S1) were generated using pCS2+sox17 and pCS2+myc-sox32 by PCR amplification using Q5 polymerase (NEB) using described primers (Table S2 ...
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bioRxiv - Microbiology 2024Quote: ... The relevant regions were amplified by PCR using primers designed for this study and shown in Table S1 Q5 High Fidelity DNA polymerase (NEB) was used for sequence analysis with appropriate purity and accuracy.
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bioRxiv - Microbiology 2021Quote: ... or 5 µl anti-H3K18cr antibody (PTM-517, PTM Biolabs) together with protein A agarose (Roche ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μl anti-MBP Monoclonal Antibody (New England Biolabs, #E8032L) and 6 μl anti-E ...
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bioRxiv - Developmental Biology 2019Quote: ... Bound antibodies were detected by incubation with anti-rabbit DyLight 800-conjugated secondary antibody (New England BioLabs). Slides were analysed using an InnoScan 710-IR scanner (Innopsys) ...
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bioRxiv - Cancer Biology 2023Quote: ... Bound antibodies were detected by incubation with anti-rabbit DyLight 800-conjugated secondary antibody (New England BioLabs). Slides were analysed using an InnoScan 710-IR scanner (Innopsys) ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and T478K of the Delta variant was spliced into the spike expression vector by HiFi DNA assembler cloning (NEB). The plasmid was prepared and verified as described above.
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bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
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bioRxiv - Molecular Biology 2020Quote: ... The four exons and three introns of Mlst8 were PCR amplified with primers listed in Table S1 and then cloned into the pCAG backbone using the Gibson Assembly Master Mix (NEB, #E2611S). Similarly ...
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bioRxiv - Cancer Biology 2020Quote: ... The guide RNA or scrambled control sequences (Supplementary Table S1) were subcloned into the lentiCRISPR v2 using the BsmBI restriction endonuclease (NEB R0580S). Virus was produced through PEI (MilliporeSigma ...
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bioRxiv - Biophysics 2021Quote: ... Mutagenic PCR to obtain the D614G amino acid change into both untagged and 161/345A4-tagged SΔTM constructs was done using the primers S2_D614_Q5-F and S2_D614_Q5-R (Table S1) and the Q5® Site-Directed Mutagenesis Kit (NEB®, Ipswich, MA, USA) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2021Quote: ... A 2.3 kb amplicon including the NAC18.1 gene and ∼800 bp of upstream sequence was amplified by PCR using primers NAC18F2 and NAC18R2 (Table S1) and Phusion® High-Fidelity PCR Master Mix with HF Buffer (NEB). PCR product size and purity was confirmed by agarose gel electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... UPF2L (120-1227) were produced by PCR amplification (primers listed in Supplementary Table S1) using the Phusion High-Fidelity PCR kit (NEB #E0553S) prior to restriction digest and ligation into pPROEX-HTB (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... and then ligated into the Bbs1 site of the vectors pX335 and pKN7 (Appendix Tables S1) in the presence of T4 DNA ligase (New England Biolabs, M202), as previously described (Pyzocha et al ...
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bioRxiv - Microbiology 2019Quote: ... Primers 5’Pcrz1 and 3’Tcrz1 (Additional file 1:Table S1) were used to amplify the deletion cassette from the yeast genome using Phusion® High-Fidelity Polymerase (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: The P562del and Exo+THR mutants were generated on the pET23-P2-D12A-THR (Table S1) background through inverse Polymerase Chain Reactions (iPCR) using Q5® High-Fidelity DNA Polymerase (NEB) following the manufacturer’s instructions in 25 μl reactions with primers p2_thumb_loop_R/DEL1 (Table S2 ...
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Enhancers display constrained sequence flexibility and context-specific modulation of motif functionbioRxiv - Genomics 2022Quote: ... Drosophila library fragments were amplified (primers see Supplementary Table S1) and cloned into Drosophila STARR-seq vectors containing the DSCP core-promoters using Gibson cloning (New England BioLabs; E2611S). The oligo library for human STARR-seq screens was amplified (primers see Supplementary Table S1 ...
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bioRxiv - Immunology 2022Quote: Point mutations were introduced through linearizing the plasmid by PCR with primers containing the intended mutations (Supp. Table S1) followed by reverting to circular plasmid with Gibson Assembly Master Mix (M5510A, NEB, USA).
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bioRxiv - Microbiology 2023Quote: ... custom adapters (Table S1) were ligated using a NEBNext DNA Library Prep Master Mix Set for Illumina (New England Biolabs, E6040S), and the transposon-genome junction was amplified with primers (Table S1 ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA libraries from at least three replicate individuals per sex for each lineage (Table S1) were prepared from gametes using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs). Mature gametophytes release gametes just after the medium renewal ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Microbiology 2021Quote: ... or murine anti-MBP monoclonal antibody (1:10,000; NEB; catalog# E8032S) in the above LI-COR blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... We used rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs), mouse monoclonal anti-CaM Kinase II α subunit antibody (05-532 ...
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bioRxiv - Immunology 2020Quote: ... RNA was then immunoprecipitated with anti-m6A antibody (New England Biolabs) overnight at 4°C with head-over-tail rotation ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Biochemistry 2020Quote: ... with anti-Knbu/Kibu as the positive control using anti-butyryllysine antibody (PTM Biolabs, Cat# PTM-301) and histone H3 as the loading control using histone H3 antibody (Santa Cruz Biotechnology ...
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bioRxiv - Physiology 2019Quote: ... gene-specific oligonucleotides targeting this region (see Table S1) were designed to amplify this predicted partial fragment using Q5 High Fidelity DNA Polymerase (New England Biolabs, Whitby, On) with whole adult female A ...
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bioRxiv - Developmental Biology 2022Quote: ... the PCR products (primers shown in Table S1) were digested with AvaII and HpyCH4IV restriction enzymes (New England BioLabs; Ipswich, MA, USA), respectively ...
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bioRxiv - Pathology 2020Quote: ... Candidate colonies were screened using external primers (Table S1) by PCR using Phusion HiFi polymerase according to the manufacturer’s instructions (New England BioLabs, Ipswich, MA, USA). Candidates with the expected PCR fragment size were sequenced using external primers to confirm the knock-out of the gene fragment.
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bioRxiv - Synthetic Biology 2023Quote: ... The pUdO#a was obtained by assembling four fragments (Table 1 and Figure S1) using the Gibson Assembly® Master Mix (New England Biolabs, E2611) according to the supplier’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... employing triple template PCR as described128 with primer sequences listed in Supplementary Table S1 using the Q5 polymerase (New England Biolabs, Ipswich, USA). The knockout constructs were released from their vector backbones with XhoI (PpAARE1) ...