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Citations for New England Biolabs :
1901 - 1950 of 10000+ citations for Mouse Switch associated protein 70 SWAP70 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and library amplification using Phusion HF PCR kit (NEB #E0553L).
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bioRxiv - Developmental Biology 2024Quote: ... the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, E7490) was employed to capture mRNA ...
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bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monarch PCR and DNA Cleanup Kit (NEB) to purify PCR products ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using Ultra II RNA library kit (NEB) and samples were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Genomics 2024Quote: ... and purified using a Monarch RNA Cleanup Kit (NEB, T2040S), both according to the Manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit HS DNA or RNA kit (Thermo) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated by using LunaScript RT SuperMix Kit (NEB) and qPCR was performed with the Luna universal qPCR Master Mix (NEB ...
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bioRxiv - Biophysics 2024Quote: ... purified using a PCR clean up kit (NEB, Cat# T1030L) and eluted in water ...
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bioRxiv - Biophysics 2024Quote: ... All mutations were constructed by using Gibson assembly kit (NEB). The signal peptide of murine immunoglobulin κ chain was used for expression of PDGFRβ and CSF-1R receptors ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated using the mRNA Isolation kit (NEB, S1550S). mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Ribosomal RNA was removed using the rRNA Depletion kit (NEB) and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 site-directed mutagenesis kit (New England Biolabs). These substitutions did not alter protein sequences ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were extracted using Monarch Plasmid Miniprep kit (NEB) and precipitated by cold ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... with NEB Next Library Quant Kit for Illumina (NEB, E7630L) and KAPA Library Quantification Kit (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using Monarch® RNA Cleanup Kit (NEB, T2040). An RNA/DNA mix was obtained by retrotranscribing 200pmol of RNA + 200pmol of the Reserve primer (5’-TTGTCGCACGTTCGGTGTCG-3’ ...
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bioRxiv - Microbiology 2024Quote: ... and purified with the Monarch RNA clean-up kit (NEB). cDNA was prepared from 1 μg of RNA ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit ...
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bioRxiv - Neuroscience 2024Quote: ... and hybridized with NEBNext rRNA Depletion Kit v2 (NEB E7400) to substantially diminish rRNA from the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... The NebNext® UltraTM II DNA library prep kit (NEB) for Illumina was used for sequencing library preparation following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... or a region in the N-terminal part (for TGDs) were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Neuroscience 2021Quote: The moPrP-eGFP_39/40 fusion construct carrying eGFP within the flexible N-terminal tail between aa 39 and 40 of mouse PrP was created using NEB® Golden Gate Assembly (New England Biolabs). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... we performed eight 100 μl PCR reactions per sample (4 μg DNA per reaction, 32 μg per mouse) using Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments ranging from 3 kb to 5 kb with 40-100 bp terminal homologies were amplified from mouse BAC RP23-51O13 with Q5 polymerase (NEB, M0491L). Approximately equal amount (100 ng ...
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bioRxiv - Systems Biology 2023Quote: ... Signals following a 1h incubation at room temperature with the appropriate HRP-conjugated secondary antibodies (anti-mouse NEB 7076S or anti-rabbit NEB 7074S) were acquired using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed three times with a PBS buffer 1X and then incubated with mouse anti-MBP (NEB, Cat# E8032L) or rabbit anti-V5 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared by amplifying the sgID-BC region from 32μg of genomic DNA per mouse using unique dual-indexed primers and the Q5 Ultra II 2x Master Mix (New England Biolabs, M0544X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: Cleavage of the MBP tag from NlGr7 was completed according to manual instructions of the pMAL protein fusion and purification system (NEB, Inc, USA). The tag (MBP ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... protease mutant S219V [24] was inserted at the 3’ end of gene encoding maltose binding protein (MBP) in pMAL-c5E vector (New England Biolabs, MA, USA) to construct pMAL-TEV vector ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl of the gRNA mixture was mixed with Cas9-NLS protein (final concentration of 5 μM. New England Biolabs, Ipswich, USA), 2M KCl (final concentration of 300mM) ...
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bioRxiv - Microbiology 2021Quote: ... 39 μl of each lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Molecular Biology 2022Quote: ... deglycosylation treatment (Dglyco) consisted of sample incubation for 3 hours at 37°C with 20 nL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per μL of sample ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or four (white shavenbaby experimental) sgRNA targeting the first or second exon (100 ng/μl) and CAS9 protein (EnGen Spy Cas9 NLS from NEB, Catalog # M0646M). The distribution of the major trichome height for the white control was normal (Shapiro-Wilk normality test W = 0.9637 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...