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Citations for New England Biolabs :
1801 - 1850 of 9609 citations for Mouse Switch associated protein 70 SWAP70 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... protease mutant S219V [24] was inserted at the 3’ end of gene encoding maltose binding protein (MBP) in pMAL-c5E vector (New England Biolabs, MA, USA) to construct pMAL-TEV vector ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl of the gRNA mixture was mixed with Cas9-NLS protein (final concentration of 5 μM. New England Biolabs, Ipswich, USA), 2M KCl (final concentration of 300mM) ...
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bioRxiv - Microbiology 2021Quote: ... 39 μl of each lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Molecular Biology 2022Quote: ... deglycosylation treatment (Dglyco) consisted of sample incubation for 3 hours at 37°C with 20 nL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per μL of sample ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or four (white shavenbaby experimental) sgRNA targeting the first or second exon (100 ng/μl) and CAS9 protein (EnGen Spy Cas9 NLS from NEB, Catalog # M0646M). The distribution of the major trichome height for the white control was normal (Shapiro-Wilk normality test W = 0.9637 ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragment ends were repaired using Quick Blunting™ kit (NEB), purified with QIAquick column (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were made following the NEBnext UltraII kit (NEB) and associated protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA libraries were prepared using the E7765L mRNA kit (NEB next). Paired-end read sequencing was performed using the HiSeq2500 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Assembly was done using NEB HiFI DNA assembly kit (NEB E5520) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) following the product instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... MeRIP was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... ARF1 point mutations were generated by Quick-change Mutagenesis kit (NEB) and KLD reactions ...
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bioRxiv - Cell Biology 2022Quote: ... and treated with the NEBNext rRNA depletion kit (New England Biolabs) to deplete the ribosomal RNA (rRNAs) ...
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bioRxiv - Microbiology 2020Quote: ... For 3’ adaptor ligation the NEBNext Small RNA Kit (E7560S, NEB) was used ...
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bioRxiv - Immunology 2021Quote: ... The adaptors from the NEBNext Multiplex Oligos Kits (NEB, Ipswich, MA) were ligated to the adenylated 3’ ends of the DNA fragments ...
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bioRxiv - Genomics 2020Quote: ... A quick Ligation Kit (New England Biolabs Inc., MA, CA#M200l) was used for ligating the insert and vector ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed using the (AMV cDNA kit, NEB, E6550S) according to the manufacturer’s protocol using random primers and 0.1-3 µg of total RNA per sample according to the experiment ...
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bioRxiv - Microbiology 2019Quote: ... and Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA) following the supplier protocols ...
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bioRxiv - Biochemistry 2020Quote: ... then RNAs were purified using a Monarch RNA Cleanup kit (NEB), or homemade equivalent buffers and regenerated columns,75 following the same rationale ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation (New England Biolabs Nest Ultra RNA library prep Kit) and single read Next generation sequencing (Illumina HiSeq 2500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and one round of rRNA depletion (NEBNext rRNA depletion kit, NEB). After ethanol precipitation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and dual indexing from the NEBNext Multiplex Oligos kit (NEB #7600) following manufacturer protocol including beads size selection for 200 bp inserts ...
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bioRxiv - Synthetic Biology 2020Quote: ... using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The YF-Cav1s effector was produced by subcloning the sequence coding aa 61–178 of human caveolin 1 (UniprotKB-Q03135 ...
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bioRxiv - Microbiology 2019Quote: ... and Monarch® Plasmid miniprep Kit (New England BioLabs, Ipswitch, MA) was used for purification of plasmid for gene expression and construction of deletion mutant ...
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bioRxiv - Biochemistry 2019Quote: In vitro translation (IVT) reactions used the PURExpress Δribosome kit (NEB) and a nanoluciferase reporter plasmid (Promega) ...
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bioRxiv - Genetics 2021Quote: ... mRNA was purified using NEB Monarch RNA cleanup kit (NEB, USA). 3 nl of 40 pg mRNA of each arm of the FusX mitoTALE-BE (left-pT3-FusXTBE-N and right-pT3-FusXTBE-C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, #E7770) following manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... DNA was purified using Monarch® PCR & DNA Cleanup Kit (NEB), using the following recommended protocol modification ...
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bioRxiv - Cell Biology 2021Quote: ... Suitable NEXTflex-6 barcodes were ligated using Quick Ligation kit (NEB) before consecutive selection of DNA fragments >100 bp and then >150-200 bp using AMPure XP beads ...
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bioRxiv - Microbiology 2021Quote: ... which was performed using Q5 Site-Directed Mutagenesis Kit (NEB, USA) with mutagenesis primers listed in Supplementary Table 2 ...
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bioRxiv - Plant Biology 2020Quote: ... Libraries were then amplified using the DNA UltraII Kit (NEB®). Libraries were sequenced on the HiSeq X platform (2 lanes ...
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bioRxiv - Biochemistry 2021Quote: ... Then the RNA was purified by Monarch RNA cleanup kit (NEB).
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bioRxiv - Neuroscience 2020Quote: ... The Q5 Site-Directed Mutagenesis Kit (New England BioLabs, cat#E0554S) and corresponding primer design tool were used to create RNAi-resistant UAS-ISWI ...
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bioRxiv - Cell Biology 2019Quote: ... and assessed for luminescence using the stabilized Gaussia luciferase kit (NEB; 50 μl of a 1:1 dilution with 18 MΩ water ...
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bioRxiv - Bioengineering 2021Quote: ... Then we used end prep and ligation kit (New England Biolabs, E7546S for End Repair/dA-Tailing module ...
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bioRxiv - Bioengineering 2021Quote: ... The mRNAs were purified using a Monarch RNA Cleanup Kit (NEB). We amplified target RNAs and mRNAs using primers that include a T7 promoter sequence on the 5′ end ...