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Citations for New England Biolabs :
1901 - 1950 of 5659 citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Regions of interest were amplified by PCR using a One-Taq Hot Start DNA-polymerase (New England Biolabs) with standard buffer and 3% DMSO and the following primers targeting exon2 (L ...
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bioRxiv - Microbiology 2022Quote: ... Detection of selected targets was performed with Luna® Universal One-Step RT-qPCR (New England BioLabs Inc.) according to manufacturers protocol using specific primers ...
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bioRxiv - Molecular Biology 2022Quote: ... This was followed by one round poly(A) purification using oligo d(T) magnetic beads (New England Biolabs). 1.5-2 µg of mRNA was fragmented to 100-150 nts using RNA Fragmentation Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA containing one copy of the SIV gene was linearized using EcoR1 (New England Biolabs, Ipswich, MA) following their restriction digest protocol (New England BioLabs ...
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and qRT-PCR were performed simultaneously using the Luna Universal One-Step RT-qPCR kit (NEB) with the Quantstudio 7 Flex (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA synthesis and RT-qPCR was carried out using Luna® Universal One-Step RT-qPCR Kit (NEB) according to the manufacturer’s instructions with 100 ng of mRNA ...
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bioRxiv - Bioengineering 2023Quote: ... cloning was achieved via one-pot restriction-ligation reactions with type IIS restriction enzymes (NEB or Thermo Scientific) and T4 DNA ligase (NEB) ...
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bioRxiv - Genomics 2023Quote: ... RNA expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: PCR was carried out using NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) or Taqman Fast Universal PCR master mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... one cell stage zebrafish embryos were injected with 50 nM EnGen® Lba Cas12a (Cpf1) (New England Biolabs) and each gRNA at a final concentration of 2 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template in 25 µl of 1x One Taq Standard Buffer (Biolabs, Ipswich, MA, USA), 0.2 mM dNTPs ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA was then directly used in the Luna® Universal One-Step RT-qPCR Kit (New England BioLabs). Primers listed in supplemental table 10.
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bioRxiv - Cell Biology 2024Quote: ... one of the immunoprecipitated samples was treated with 400 units of Lambda protein phosphatase (New England Biolabs, P0753S) at 30 °C for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... 1μg RNA was incubated with 1 unit XRN1 (New England Biolabs) or water for 2hr at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 unit Phusion DNA Polymerase (New England Biolabs; Ipswich, MA, USA), and 30-50 pg template ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μL of dNTPs (final concentration 0.5 mM, New England BioLabs), 0.5 μL of Taq polymerase (corresponding to 2.5 units ...
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bioRxiv - Genetics 2021Quote: ... 1 µl precipitated DNA sample and 0.3 U Q5 polymerase (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... 1 nl of a solution containing 10µM EnGen Cas9 NLS (NEB) and 100 ng/µl of gRNAs was injected at the one-cell stage ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 U per microliter murine RNase inhibitor (Biolabs ref: MO314S). Ribosome-protected fragments (RPF ...
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bioRxiv - Genomics 2020Quote: ... with 1 μl (20 U/μl) of DpnI restriction enzyme (NEB) in a final volume of 20 μl at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... 72°C for 1 min)) using NEBNext 2x Master Mix (NEB) and Nextera adapters and put on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... The first duplicate was deglycosylated by PNGase F (NEB, 1:100) overnight at 37°C in 50 mM ABC prepared in pure H2O18 ...
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bioRxiv - Molecular Biology 2020Quote: ... washed once with 400 μl 1× Quick ligation buffer (NEB, M2200) and resuspended in 46.5 μl of 1× Quick ligation buffer (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... 1% SDS) containing 400 μg/mL Proteinase K (New England Biolabs) and incubated at 65 °C for 1 hr with rotation at 300 RPM ...
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bioRxiv - Microbiology 2021Quote: ... or murine anti-MBP monoclonal antibody (1:10,000; NEB; catalog# E8032S) in the above LI-COR blocking buffer ...
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bioRxiv - Cell Biology 2019Quote: ... reaction buffer containing 1 μL (400 units) lambda phosphatase (P0753S, NEB), reaction buffer containing 1 μL lambda phosphatase plus 1×phosphatase inhibitors cocktail (Roche ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1 µl of T4 DNA ligase buffer 10X (NEB cat. B0202), 0.5 µl of 1 mg mL−1 purified bovine serum albumin (1:20 dilution in dH2O of BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μL of Universal PCR Primer (25 mmol) (NEB, Ipswich, USA), and 1 μL of Index (X ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 μL of T4 polynucleotide kinase (New England Biolabs, M0201S) for 45 min at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 µL 10x T4 DNA ligase buffer (New England Biolabs (NEB)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 µL 10x T4 DNA ligase buffer (New England Biolabs (NEB)) ...
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bioRxiv - Molecular Biology 2020Quote: ... the gel pieces were submerged in 1× DNase I buffer (NEB) and 4 U DNase I (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... 1× final concentration of the isothermal amplification buffer (New England Biolabs), 1.025 mM each of deoxy-ribonucleoside triphosphates (dNTPs) ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... supplemented with 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA) for 1 h at room temperature to allow binding of cognate proteins ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... supplemented with 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA) for 1 h at room temperature to allow binding of cognate proteins ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... supplemented with 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA) for 1 h at room temperature to allow binding of cognate proteins ...
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bioRxiv - Microbiology 2021Quote: ... using NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, NEB) and sequenced on an Illumina Novaseq 6000 (2×150 bp ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µl of lambda protein phosphatase (New England BioLabs, Ipswich, MA). Each mixture was incubated at 30 °C for either 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... washed with 100 μl of 1 × NEBuffer 2.1 (NEB; Cat#: B7202S), and resuspended in 50 μl of 1 × NEBuffer 2.1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... 12 mM MgCl2 and 1 U/ μl T7 Polymerase (all NEB). The transcription mix was incubated at 37°C for 4 h ...
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bioRxiv - Plant Biology 2021Quote: ... 1 U of Q5 High-Fidelity DNA polymerase (New England Biolabs), and 1 μl of DNA ...
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bioRxiv - Microbiology 2021Quote: ... followed by the addition of RNase III (NEB; 1/625 U) and further incubation at 37°C for 5 minutes to allow limited cleavage ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were ligated by T4 RNA ligase 1 (New England BioLabs), and purified by ethanol precipitation ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50 µl T4 RNA ligase 1 (NEB, catalog no. M0204). Bead complexes were then washed 3 times with 1 mL PNK buffer ...
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bioRxiv - Systems Biology 2022Quote: ... Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs) or Quick-Load® 1 kb Extend DNA Ladder (New England Biolabs ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs), 1 µl of BsaI-HFV2 (New England Biolabs) ...
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bioRxiv - Biophysics 2022Quote: ... for POT1 or 1 mL amylose beads (New England BioLabs, E8021S) for shelterin for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μL 10 mM ATP (New England BioLabs; Ipswitch, MA, USA), 10 units T4 RNA Ligase 1 (New England BioLabs ...
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bioRxiv - Molecular Biology 2022Quote: Isothermal products (1 μL) were digested with AvaII (New Englanc Biolabs) according to manufacturer’s instructions to confirm template specific origin (Supplementary figure 1) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were fixed in 1:3 glacial acetic acid:methanol (Biolabs-chemicals) solution and the G-banding karyotype was determined ...