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Citations for New England Biolabs :
1751 - 1800 of 5659 citations for 7 Oxa 1 2 diazaspiro 4.4 non 1 en 6 one 4 methyl cis 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 1 μL T4 DNA Ligase (2,000,000 U/mL, NEB, #M0202M), 1 μL BsaI-HF v2 (NEB ...
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bioRxiv - Immunology 2023Quote: ... 1 mM NAD+ in 1x rCutSmart buffer (New England Biolabs). The reaction was incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were digested with 1 μL DpnI (NEB #R0176L) for 1 hour and purified by agarose gel extraction (MinElute Gel Extraction Kit ...
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μl anti-MBP Monoclonal Antibody (New England Biolabs, #E8032L) and 6 μl anti-E ...
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bioRxiv - Developmental Biology 2023Quote: ... adding 400 U (1 μl) T4 DNA Ligase (NEB, #M0202S), and incubating the reactions at 16 °C for an additional 5-16 h.
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bioRxiv - Immunology 2023Quote: ... with the addition of 1 µL GluC protease (P8100S, NEB) at 1 mg/mL per mg of RH5ΔNL (1:1000 wt/wt) ...
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bioRxiv - Biophysics 2023Quote: ... and 1 μL of BaeGI-HF (NEB; 100,000 units/mL) and incubated for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... final 25% PEG8000 and T4 RNA ligase 1 (NEB, M0204L). The ligation reaction was incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 1 μL of 10 mM ATP (NEB, cat. no: M0204S), 1 μL of 10 μM M13 Ligation primer ...
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bioRxiv - Molecular Biology 2023Quote: ... 40 units of T4 RNA Ligase 1 (New England Biolabs) in a total volume of 80 µl ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 µL PCR reaction contained 1× OneTaq Master Mix (NEB), 0.2 µM of each primer ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 kb and 100 bp DNA Ladders (New England Biolabs) were used to measure amplicon size ...
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bioRxiv - Cell Biology 2023Quote: ... in 1× T4 DNA ligase buffer (New England Biolabs, B0202S) containing 1% TritonX-100 and for 4 hours at 16°C followed by additional incubation for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM TCEP and 2.5 units/mL of YIPP (NEB). At each time point reaction was quenched with 4X LDS and 50 mM DTT then run on SDS-PAGE ...
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bioRxiv - Biophysics 2023Quote: ... 1 mM TCEP and 2.5 units/mL of YIPP (NEB). RNA-nsp9 product was then purified from the protein and leftover RNA components through a 1 mL HiTrap Q Fast Flow column in a buffer of 20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 μl of BsaI enzyme (New England Biolabs, USA). The PCR program was ...
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bioRxiv - Molecular Biology 2023Quote: ... enzyme) in the reaction buffer containing 1 × Cutsmart buffer (NEB), 2 mM ATP (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was incubated with 1 U T4 polynucleotide kinase (NEB) in presence of its buffer (70 mM tris-HCl pH 7.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1× ProtoScript II RT Reaction Buffer (New England Biolabs) was incubated for 1 h at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... 50 µM ATP and 1 x RNA ligation buffer (NEB) at 25 °C for 16 h ...
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bioRxiv - Genomics 2024Quote: ... 1 µL of M.TaqI (10 units/µL; New England Biolabs) and ultrapure water in a total volume of 40 µL ...
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bioRxiv - Microbiology 2023Quote: ... and 1 unit/µL of T4 DNA Ligase (NEB #M0202). A no-insert with backbone only control was prepared in parallel with each ligation reaction to serve as a backbone self-ligation control ...
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bioRxiv - Molecular Biology 2024Quote: ... 40 µM FeSO4 and 1 µl murine RNase inhibitor (NEB). The products of the reactions were digested to nucleosides and analyzed by LC-MS/MS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μL of T4 PNK (New England Biolabs,M0201S) in Ultrapure water (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µL of T4 DNA ligase (New England Biolabs). Following ligation ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 U of Q5 HotStart Taq polymerase (New England Biolabs) in 1 X Q5 buffer ...
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bioRxiv - Plant Biology 2024Quote: ... The sample was supplemented with 1 µL RNase H (NEB) and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... was amplified as per the ChIPmentation protocol [64] using indexed and non-indexed primers and NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541) in a thermomixer with the following program ...
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bioRxiv - Biochemistry 2021Quote: ... The amplified templates were used for in vitro transcription with recombinant T7 RNA polymerase in the presence of either m7GpppG cap analog or non-functional cap analog ApppG (New England Biolabs®). To check RNA integrity ...
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bioRxiv - Microbiology 2022Quote: ... The resulting double-stranded oligos had 5’ extensions that were complementary to the non-palindromic 3’ overhangs generated by BsaI-HFv2 (NEB, R3733) digestion of pJJW101 ...
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bioRxiv - Molecular Biology 2023Quote: F1 dumpy and/or non-dumpy animals were isolated from dumpy jackpot plates and genotyped by PCR and restriction digestion using HpyCH4IV (F180Δ, NEB R0619), HinfI (G199S ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified cDNA was converted to double stranded DNA (dsDNA) by performing second strand synthesis with NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (NEB, E6112) in 20 µl reaction volume for 2.5Lh at 16L°C and 20 min at 65L°C ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR addition of indices (7-11 cycles) was done using NEBNext Ultra II DNA library prep kit (NEB). Biotinylated capture probes 70 nt in length were designed against every DpnII restriction fragment in a 2.5 Mb window centered on Runx1 (chr16:91,566,000-94,101,999) ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA of RIN (RNA integrity number) more than 7 is subject to NEBNext Small RNA library kit (NEB). After final PCR amplification with indexed-adaptors ...
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bioRxiv - Molecular Biology 2021Quote: ... The Taq I-digested chromatin was diluted 7-fold to which 10,000 cohesive end units of Quick T4 DNA ligase (New England Biolabs) were added ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... asynchronous or mitotic HeLa cell lysates were incubated with 0.9 mM MnCl2 and 7 U/µl λPP (New England Biolabs) or 0.9 mM MnCl2 and water for 1 h at 30 °C.
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bioRxiv - Cancer Biology 2023Quote: ... the region of interest was minimally amplified through a 7-cycle PCR (Phusion® High-Fidelity DNA Polymerase, NEB) surrounding the mtDNA regions for ddPCR detection ...
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bioRxiv - Genomics 2024Quote: ... 30 µL of the eluted DNA samples were mixed with 7 µL of TSO digestion mix (3.5 µL UDG-DNA glycosylase and 3.5 µL UDG Buffer, NEB M0280S) and incubated at 37 ºC for 30 min ...
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bioRxiv - Genetics 2020Quote: ... followed by gap repair by adding 2 µl of 10× NEBuffer 2 (NEB), 3 µl of dNTPs (2.5 mM each ...
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bioRxiv - Molecular Biology 2021Quote: ... and 2’O-methylated using Vaccinia VP39 (2’O Methyltransferase) (New England Biolabs), then purified by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Genomics 2020Quote: ... One microgram of the sonicated DNA was incubated in 1x T4 DNA ligase buffer (NEB; Ipswich, MA) with components ...
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bioRxiv - Microbiology 2019Quote: ... we performed q-RT PCR using the Luna Universal One-Step RT-qPCR Kit from NEB (E3005), using the Applied BiosystemsΤΜ 7500 Real-Time PCR system ...
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bioRxiv - Synthetic Biology 2019Quote: ... All Gibson reactions were performed at 50 °C for one hour with T5 exonuclease (New England Biolabs), Phusion polymerase and Taq ligase (New England Biolabs) ...
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bioRxiv - Microbiology 2019Quote: ... RT-PCR was performed in a 50 µl reaction mixture with One Taq 2x master mix (NEB), 20 ng of cDNA and 0.2 µM of each primer (Sigma) ...
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bioRxiv - Genomics 2021Quote: One reaction of NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645) was used for 1 μg of input DNA ...
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bioRxiv - Biochemistry 2020Quote: ... One microgram of total RNA was reverse transcribed into cDNA with Protoscript II (New England Biolabs, M0368L) followed by qPCR with SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... The amplified PCR product was digested in one step using NdeI and BamHI HF (New England Biolabs) and ligated into similarly treated pET-15b to create plasmid pET15b-EcGhrA ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR kit (New England Biolabs; #E3005L). Briefly ...