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Citations for New England Biolabs :
1901 - 1950 of 7574 citations for 7 Chloro 1 3 dihydro 1 methyl 5 phenyl 2H benzo 1 4 diazepin 2 one 4 oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 5 uL aliquots were removed at each time point and added to 2 uL 5 mg/mL Proteinase K (NEB) in 5% SDS ...
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bioRxiv - Microbiology 2020Quote: Reverse transcription and amplification for figure 2 was performed using OneTaq One-Step RT-PCR Kit from NEB (cat. # E5315S). Both the OneTaq One-Step RT-PCR Kit and Luna Universal One-step RT-qPCR Kit (cat ...
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bioRxiv - Plant Biology 2021Quote: ... One μl of each 100 μM oligo was added to 500 μl 1x NEB buffer 2 (New England Biolabs, www.neb.com). The p201N:Cas9 plasmid was linearized by digestion with Spe1 (www.neb.com ...
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bioRxiv - Microbiology 2024Quote: Quantification of SARS-CoV-2 E gene subgenomic mRNA (sgmRNA) was conducted using Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a Step One Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 µg of empty vector and 1 µg of purified amplicon were digested overnight with 1 µl of SphI-HF® (New England Biolabs, catalog no. R3182) in a total volume of 50 µl ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... and 15ul BseYI-digested product were run side-by-side on gel (1% agarose gel in TAE buffer, 1 kb ladder (NEB, Cat. No. / ID: N3232L), most gels run at 115V for 40 minutes) ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Genomics 2023Quote: ... The DNA length and RNA absence were assessed by 2% agarose gel electrophoresis (50 V, 90 min) including the Quick-Load 1 kb DNA Ladder (New England Biolabs Inc., Ipswich, USA; Text S1). The extracted gDNA was not fragmented and larger than 10 kb (Fig ...
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bioRxiv - Microbiology 2022Quote: ... 0.75 µl End-prepped DNA, 1 µl Native Barcode (Native Barcoding Expansion 96, EXP-NBD196) and 5 µl Blunt/TA Ligase Master Mix (NEB Blunt/TA Ligase Master Mix, M0367) were combined in a new reaction vessel and incubated for 20 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The samples were converted for sequencing with the NEBNext enzymatic methyl-seq kit (NEB) and sequenced at the University of Minnesota Genomics Center ...
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bioRxiv - Genomics 2023Quote: ... We used the NEB Next Enzymatic Methyl-seq Kit (New England Biolabs Cat.no. E7120S). Control DNA (CpG methylated pUC19 and unmethylated lambda ...
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bioRxiv - Genetics 2023Quote: ... digested DNA was converted using New England Biolabs’ Enzymatic Methyl-seq Kit (E7120S, NEB) to detect selectively oxidized unmethylated cytosines ...
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bioRxiv - Molecular Biology 2024Quote: ... End prep and adaptor ligation was performed using the Enzymatic Methyl-seq kit (NEB). Conversion was performed identically to the amplicons ...
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bioRxiv - Genomics 2021Quote: ... One μL APOBEC3A (NEB #E7120S) was added directly to the reaction with 10 μL of 10x APOBEC3A reaction buffer and 1 μL BSA (10 mg/mL) ...
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bioRxiv - Cell Biology 2023Quote: ... and one in-solution (NEB) DNase digestions were performed ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted in 1% low melting point agarose (LMP) (Biolabs) within a 1mm diameter glass capillary ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Physiology 2022Quote: ... anti-MBP (1:10,000, catalog no. E8032S, New England Biolabs); anti-CCNA2 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Excess adapter was digested by adding 1 µl RecJf (NEB) and 1 µl 5’deadenylase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-GFP (1:1000; TP401, Torrey Pine Biolabs, RRID:AB_10013661), mouse anti-GFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and 1 unit of poly(A) polymerase (New England Biolabs) and was incubated at 42°C for 1 hour followed by enzyme inactivation at 95°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... 0.1mM dNTPs and 1 U/μl USER enzyme (NEB, USA). 75μl of the mix was added and incubated for 3h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... and dNTPs in 1× T4 DNA ligase reaction buffer (NEB), followed by dATP-addition with Klenow ...
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bioRxiv - Microbiology 2020Quote: ... 1× Phusion HF buffer (New England Biolabs GmbH, Schwalbach, Germany) with 1.5 mM MgCl2 ...
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bioRxiv - Genomics 2019Quote: ... and various amounts of the 1 kb ladder (NEB#N3232s) were run on 1% Agarose gel.
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bioRxiv - Cell Biology 2019Quote: ... 1% NP40 and PNGase-F (50 U/μl: NEB#P0704S).
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bioRxiv - Genomics 2019Quote: ... 34 µl of H2O and 1 µl of PstI (NEB) were added and restriction digestion was performed at 37°C for 4 h ...
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bioRxiv - Genomics 2021Quote: ... and 1 µl of PNK (NEB M0201, 10,000 units/ml) and incubated at 37 °C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... were ligated using 1 μl Quick T4 DNA Ligase (NEB) and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC ...
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bioRxiv - Genomics 2020Quote: ... 1 µl Calf Intestinal alkaline Phosphatase (10 U; CIP, NEB) or quick CIP (10 U ...
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bioRxiv - Cell Biology 2020Quote: ... 2700 units mL−1 murine RNase inhibitor (New England Biolabs), and 0.1 M Tris-HCl (pH 8.0)).81 Primers consisted of a poly-T sequence to trap mRNA molecules ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... and 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA). Labeling reactions were incubated for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM sodium vanadate (New England Biolabs, Ipswich, MA, #0758s); 50 mM sodium fluoride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions were treated with Exonuclease 1 (New England Biolabs, M0293S) for 45 minutes at 37℃ ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pulse-labeled with SNAPcell-505 (1 µM; NEB) for 20 min in media ...
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bioRxiv - Genomics 2021Quote: ... 0.5 μL of 1 mg/mLbovine serum albumin solution (NEB), 1.2 μL of 50 mM NAD+ (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μL DpnI enzyme (20 U, New England Biolabs #R0176L) was added to the 25 μL PCR mixture immediately after the final extension and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: 1 U (0.5 μl) RNaseIII (NEB Short Cut RNase III), was combined with 1 μl of 10X reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 U/μL exonuclease III (New England BioLabs®), while the other half of each cleanup was not treated ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl microccocal nuclease (New England Biolabs, Ipswich, MA, USA) and 2 μl benzonase (Merck-Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μl of T4 DNA ligase buffer (NEB, Ipswich, MA), 0.5μl of restriction enzyme (BsaI-HFv2 or Esp3I) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Lane M is DNA marker (NEB 1 Kb DNA Ladder), lane N shows the PCR product from negative control (ddH2O) ...
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bioRxiv - Developmental Biology 2020Quote: ... once with 1 mL NEBNext dA-Tailing reaction buffer (NEB) and then resuspended in 50 μL of with NEBNext dA-Tailing reaction buffer (NEB ...