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Citations for New England Biolabs :
1801 - 1850 of 3219 citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and M-MuLV reverse transcriptase enzyme (200,000 U mL−1, New England Biolabs, Ispwich, USA) was added per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then probed with the primary anti-MBP antibody (NEB E8032S, 1:10000) diluted in 5% NFDM (overnight ...
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bioRxiv - Biochemistry 2023Quote: Extracted samples (1 μL) were digested with NotI-HF (0.89 unit/μL, New England Biolabs) and ClaI (0.44 unit/μL ...
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bioRxiv - Cancer Biology 2023Quote: ... digested overnight at 37°C with 1 μl 20 U/μl NotI-HF (NEB R3189S) and 1 μl 10 U/μl PmeI (NEB R0560S ...
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bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... the isolated RNA was treated with 1 μl (5 units) RppH enzyme (New England Biolabs) in NEB Buffer 2 (50 mM NaCl ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg RNA was reverse transcribed to cDNA (LunaScript RT SuperMix, New England BioLabs, USA).
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bioRxiv - Physiology 2023Quote: ... 1 U of Phusion® Hot Start II High-Fidelity DNA polymerase (New England BioLabs), 500 nM of the 27F-DegS primer ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 in the presence of 25 mU ml-1 apyrase (NEB, Cat. no. M0398S) and 1 µM of individual ligand ...
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bioRxiv - Biochemistry 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to the annealed product ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs). Agarose gel was electrophoresed at 70V until resolution of bands required was reached ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added and incubated at 25 °C for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... PCR amplified Domain 1 and PCR Domain 2 and HiFi DNA assmbley was performed (NEB). Transformation was performed in DH5α cells (REF) ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Plugs were rinsed with TE and then washed with 1 ml NEB buffer 4 (NEB) 3 × 15min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µL of T4 DNA ligase high-concentration (2,000,000, units/mL, NEB, cat. no. M0202T) was added ...
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bioRxiv - Immunology 2023Quote: 200 ng extracted mRNA was digested into nucleosides by Nuclease P1 (1 U, NEB, M0660S) and shrimp alkaline phosphatase (rSAP ...
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bioRxiv - Developmental Biology 2023Quote: ... After samples were PCR-amplified using 1×NEBNext High-Fidelity PCR Master Mix (NEB, M0541S). Subsequent libraries were purified with the MinElute PCR Purification Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... at 37 °C for 1 hour prior to the addition of T4 DNA ligase (NEB). The ligation reaction was incubated at room temperature for 1 hour then transformed into DH5ɑ competent cells.
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bioRxiv - Genetics 2024Quote: ... A mixture of 20 ng/μL transgene and 70 ng/μL 1 kb ladder (NEB) filler DNA was microinjected into CZ26711 young adult hermaphrodites ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step two plasmid library was linearized with SpeI-HF (NEB #R3133) at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... RNA samples (1 µg each) were mixed with 2x RNA Loading Dye (New England Biolabs), denatured at 72 °C for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of one plasmid was digested overnight with 10 units PacI (New England Biolabs) in 20 μL at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Successful reactions were directly incubated at 37 °C with 1 µL of DpnI (NEB, #R0176L) for 30 min to 3 hrs ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA substrate was incubated with 1 unit of purified Uracil-DNA glycosylase (New England BioLabs) instead of cell extracts in the same reaction buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we added 1 uL of T4 DNA ligase buffer (New England Biolabs, USA, product #B0202S). Using a thermal cycler (C1000 Touch Thermal Cycler ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction system was as follows: 20 μL volume containing 1× reaction buffer (NEB, B9012S), 5 U/μL T7 RNA polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 1.35 μL of 1 mM dNTPs and 0.8 μL Klenow exo-polymerase (New England Biolabs) were added to fill in the barcode oligo ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Microbiology 2024Quote: ... 0.4 µg μl-1 bovine serum albumin (New England Biolabs® Inc., Ipswich, MA, USA) and 5 µl DNA template ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was stopped by adding 1 µL of Thermolabile Proteinase K (NEB, Ipswich, MA) and incubating for 30 min at 37°C then an additional 10 min at 60°C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of the sgRNA was complexed with 20 pmol recombinant Cas9 endonuclease (NEB # M0646T) and injected into the C ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this was then combined with 5 μL Q5 buffer (NEB, cat#M0491 S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... or a polyclonal rabbit antibody against cleaved casp-3 (1:100; New England Biolabs, Frankfurt, Germany), followed by a biotinylated goat anti-rabbit secondary antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 10 mM dATP and 15 units of Klenow 3’ → 5’ exo- (NEB M0212L) was added to blunted ends and incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5’ adaptor (rGrUrUrCrArGrArGrUrUrCrUrArCrArGrUrCrCr-GrArCrGrArUrCrNrNrNrCrGrArNrNrNrUrArCrNrNrN, N is for a random nucleotide) by T4 ligase 1(NEB) sequentially ...
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bioRxiv - Genomics 2022Quote: ... The electroporated bacteria was immediately split into ten 1 mL aliquots of outgrowth medium (NEB, B9035S) and incubated at 37°C for 1 hr then independently scaled up in 20 mL of LB supplemented with 100 μg/mL of carbenicillin (Teknova ...
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bioRxiv - Genomics 2020Quote: ... and barcoded with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England Biolabs). We sequenced prepared libraries on an Illumina MiSeq using MiSeq Reagent Kit v3 (2×300 cycles ...
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Genomics 2020Quote: ... All ligations were supplemented with 1:40 RNAse inhibitor (ThermoFisher Ribolock or NEB Murine RNase Inhibitor) to prevent RNA degradation.
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bioRxiv - Molecular Biology 2019Quote: ... The Cap 1 25mer RNA was purified using Monarch RNA Cleanup kit (50 μg capacity, NEB). Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Bioengineering 2019Quote: ... The inserts were ligated to the phPB52 digested vector in 1× T4 DNA ligation buffer (NEB) with 30 ng of vector DNA with three molar excess of synthetic inserts ...
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bioRxiv - Molecular Biology 2019Quote: ... We used the NEBNext Multiplex Oligos for Illumina (Index Primers #1 - #24) (NEB, E7335S and E7500S) as the unique index primers ...
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bioRxiv - Microbiology 2019Quote: ... 1 μl Exonuclease I and 2.44 μl Antarctic Phosphatase buffer (New England BioLabs GmbH, Frankfurt, Germany) to 20 μl of the pooled PCR product ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...