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Citations for New England Biolabs :
1801 - 1850 of 3754 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented RNA was then purified and circularized with T4 RNA ligase 1 (NEB). Tandem repeats were generated during first-strand synthesis at 42 °C for 30 min ...
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bioRxiv - Genetics 2020Quote: ... and 0.3 μL quick ligase in 1 x quick ligase buffer (New England Biolabs) with a total volume of 6 μL ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... 1 μL deoxynucleotide Solution Mix and 0.5 μL Taq DNA Polymerase (New England Biolabs). The reactions were incubated in an Eppendorf Mastercycler Gradient Thermal Cycler for 7m at 95°C ...
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bioRxiv - Biochemistry 2021Quote: ... one of which was incubated with 1 µl nicking enzyme (10 units Nt.BspQI (NEB) or 5 units Nb.Bpu101 (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... reaction products were resuspended in 50 µl relevant manufacturer’s enzyme buffer (1 × Cutsmart (NEB) for nicking with Nt.BspQI or 1 × Buffer R (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.02% NP-40) including 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... The chambers of the microplate were pre-treated with 1 mg/ml BSA (NEB) for 30 min before aspirating the BSA ...
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bioRxiv - Immunology 2020Quote: ... enzymatic digestion was performed 30 min with 1 unit T7 endonuclease (New England Biolabs) at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Digestion was performed for 1 h using Cut Smart Buffer (NEB, Cat no. B7204S). Next ...
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bioRxiv - Biophysics 2020Quote: ... The flow cell was incubated for 10 minutes with 1 mg/ml streptavidin (NEB) and then 10 minutes with Blockaid (ThermoFisher) ...
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bioRxiv - Bioengineering 2022Quote: ... Each 10 µL experiment contained: 1 µL 10x isothermal buffer (New England Biolabs (NEB)) ...
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bioRxiv - Bioengineering 2022Quote: ... Each 10 µL experiment contained: 1 µL 10x isothermal buffer (New England Biolabs (NEB)) ...
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bioRxiv - Biophysics 2022Quote: ... fluorescently labeled dCas9 was incubated with gRNA in 1× NEBuffer 3.1 (New England Biolabs). 1 nM dCas9 was incubated with 10 nM gRNA for 30 min at 37 °C on an orbital shaker ...
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bioRxiv - Bioengineering 2022Quote: ... we used a 1 kb Plus DNA Ladder for Safe Stains (NEB; catalog # N0559S), Gel Loading Dye ...
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bioRxiv - Microbiology 2022Quote: ... two complementary oligonucleotides (1 μM each) were hybridized in T4 DNA-ligase-buffer (NEB) containing T4 polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2022Quote: ... To 200 μL of supernatant 1 μL DNase (New England Biolabs, catalog number: M0303S) 1 μL RNaseA (Biomed ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... (1) DNA fragments were repaired with the End-Repair enzyme mix (New England Biolabs). (2 ...
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bioRxiv - Biophysics 2022Quote: ... diluted to ∼1 nM in 1x T4 DNA ligase reaction buffer (New England Biolabs), and treated with T4 DNA ligase to circularize the DNA ...
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bioRxiv - Plant Biology 2024Quote: ... was used for Level 1 and 3 assembly and SapI (New England BioLabs, USA) for Level 2 assembly ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested and resuspended with 1:10 volumes of CutSmart Buffer (NEB, # B7204) and 5 µl of 100 mg/ml RNase-A solution (pre-heated at 100°C for 20 minutes) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA) with or without Short Cut RNase III (New England Biolabs; 1:150) for 90 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step one plasmid library was linearized with XhoI (NEB #R0146) at 37°C for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... The digested nuclei were washed and resuspended by 1 x T4 ligase buffer (NEB). Nuclei were then ligated for 4 h at room temperature by adding 1% Triton X-100 ...
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bioRxiv - Biophysics 2023Quote: 12mer arrays (40 ng) were digested with 1 U of MNase (New England Biolabs) in a 10 μL reaction for varying digestion times (2.5 to 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... these products were ligated to a 3’ adapter (1× T4 RNA ligase buffer (NEB), 10% PEG-8000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Rabbit polyclonal anti-SNAP-tag (Cat no. P9310S, New England Biolabs, 1:1000 dilution). Secondary antibodies used were ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1; New England Biolabs) were used as specified by the manufactureŕs instructions ...
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bioRxiv - Microbiology 2023Quote: ... For XRN1 treatment refolded RNA (1 μg) was incubated with 1U XRN1 (NEB, USA) and 10U RppH (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... this was followed by incubation with 1 µl PNGase F (NEB, Ipswich, MA, USA), 2 µl 10% NP-40 (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... Library indexing was done with NEBNex multiplex oligos for Illumina Set 1 (E7335S, NEB) and Set 2 (E7500S ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL RNAs were mixed thoroughly with 1 μL T4 PNK (10 U, NEB), 1 μL purified TS2126 and 1 μL PAP1 enzyme (NEB ...
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bioRxiv - Cell Biology 2022Quote: 1 μg of total RNA was reverse transcribed with Protoscript II reverse transcriptase (NEB), both according to the manufacturer’s instructions using random hexamer primers ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... The membrane was incubated with HRP-conjugated (1/2,500 New England Biolabs, Ipswich, MA) or IRDye® (1/10,000 LI-COR Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... The resulting reaction product was mixed with 1 μl Exonuclease V (New England Biolabs) and 3 μl of 10 mM ATP in NEB Buffer 4 ...
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bioRxiv - Genetics 2023Quote: ... 5% DMSO and 1× NEBNext High-Fidelity PCR master mix (New England BioLabs, M0541L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... were added in a total of 50 µl 1× CutSmart buffer (New England Biolabs). The reaction was run for 15 min at 37 °C on a thermocycler ...
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bioRxiv - Genomics 2023Quote: ... cDNA (1 µg) was prepared from total RNA using LunaScript cDNA synthesis mix (NEB), diluted 5 times before using it for qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... The 385 bp PCR product was digested with 1 unit of Hpy188III (NEB #R0622) for 2 hrs at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Streptavidin conjugated DNA was ligated to the appropriate concentration adapter (1:10) (NEB, 7335L). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 1X DNase I reaction buffer with 1 µL DNase I (NEB) for 15 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (E7335; NEB) according to the manufacturer’s protocols ...