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Citations for New England Biolabs :
1701 - 1750 of 3754 citations for 1 1 Dimethylsila 11 Crown 4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1 unit of Phusion Polymerase were mixed with 1x HF Phusion buffer (NEB) and made up to 50 µl total volume with H20 ...
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bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
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bioRxiv - Systems Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB EE7600S), and an Illumina NextSeq 550 75-cycle high-output kit (Illumina #20024906) ...
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bioRxiv - Biochemistry 2023Quote: ... The final Golden Gate reaction volume contained 1× concentrated T4 ligase buffer (NEB), 250 ng Golden Gate vector (pcDNA3-based carrying a twin Strep-FLAG tag ...
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bioRxiv - Genetics 2023Quote: ... the rRNA was eliminated by adding 1 μl RNase H (New England Biolabs), 4 μl 10X RNase H buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton X-100) supplemented with Proteinase K (1:100, New England Biolabs). Specimens were incubated on an orbital shaker at 37°C per 2 hours and 60 rpm.
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bioRxiv - Biophysics 2023Quote: ... The left lane (“L”) has a DNA size ladder (1 kb+, NE Biolabs). The black arrow in the left lane (“P” ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB, #E7600S) with primer indexing PCR run according to the Epicypher protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μl were combined with 1 μl 10X T4 Ligation Buffer (NEB, M0202), 6.5 μl Nuclease-free H2O and 0.5 μl T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Microbiology 2023Quote: ... RNA concentration was determined by nanodrop and DNase 1 treated (NEB, Cat. # M0303L). DNase 1 (4 units ...
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bioRxiv - Developmental Biology 2023Quote: ... 1μg RNA was incubated with either 1 unit of XRN1 (New England Biolabs) or water for 2hr at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... were ligated using NEBNext DNA Sample Prep Reagent Set 1 (New England Biolabs). Immunoprecipitations were carried out using 500ng DNA per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl of 10x T4 RNA ligase 2 (truncated) buffer (New England Biolabs), 3 μl of 50% PEG 8,000 (New England Biolabs) ...
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bioRxiv - Systems Biology 2024Quote: ... then digested for 1 hour at 37 °C using SacI (New England Biolabs). This linearized plasmid was used for Gibson assembly with an insert composed of a 248 base pair region of PBS2 amplified from genomic DNA ...
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bioRxiv - Genetics 2024Quote: ... PCR-1 and PCR-2 amplicons were digested with DpnI (NEB Cat#R0176S) for 2 hours at 37°C to remove the MSp509_PKR-WT template DNA ...
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bioRxiv - Cell Biology 2024Quote: ... then 1 μL Endo H and 2.5 μL GlycoBuffer 3 (New England Biolabs) was added and incubated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 founder with the L424V was determined by restriction digest with MseI (NEB) and Sanger sequence analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µl of tailing mix (0.05 µl Terminal Transferase (NEB, cat. no. M0315), 0.1 µl 10x Cutsmart buffer ...
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bioRxiv - Biochemistry 2024Quote: ... 1× Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), 0.5 µM i5 index primer ...
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bioRxiv - Microbiology 2024Quote: ... the chamber was blocked with 1 mg/ml bovine serum albumin solution (NEB) for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... mixed with 1 μL (10 units) of T4 Polynucleotide Kinase (NEB, Cat #M0201L), 5 μL T4 Polynucleotide Kinase Reaction Buffer (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µL of the BsaI-HF®v2 restriction enzyme (NEB, R3733S) (Figure 1F) ...
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bioRxiv - Genomics 2024Quote: ... was utilized using 1 µg gDNA and the pre-annealed NEB adapter (NEB_P7 ...
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bioRxiv - Immunology 2024Quote: ... 1% penicillin/streptomycin (100U/mL penicillin and 100 µg/mL streptomycin, Nordic Biolabs), and supplemented with 1mg/mL geneticin (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... the chamber was blocked with 1 mg/ml bovine serum albumin solution (NEB) for 5 min ...
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bioRxiv - Immunology 2024Quote: ... 1 µl of the recombinants was transformed into NEBstable competent cells (NEB, USA). Bacterial cells were spread on agarose plates ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM phosphoenolpyruvate and 0.15 mg/ml bovine serum albumin (New England Biolabs), in a final volume of 15 µl ...
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bioRxiv - Microbiology 2024Quote: ... and 1/10th volume of DNAse I reaction buffer (NEB cat. no. B0303S) were added to 1 mL of phage lysate and incubated at 37°C for 30 min followed by a 60 min incubation at 65°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 nM DNA template and 1.25 U/μL T7 RNAP (New England Biolabs) were mixed in 100 μL transcription buffer containing 40 mM Tris-HCl (pH 8.5) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was initiated by adding 1 mM NTP mix (New England Biolabs). Cy3 and Cy5 were excited with light at λex=540±18 nm and λex=640±20 nm ...
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bioRxiv - Biophysics 2024Quote: ... PM187B20 was 5’ end labelled using 1 μL T4 polynucleotide Kinase (NEB, M0201S), 1 μL γ 32P ATP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR-1 was performed with Q5 Hot Start High-Fidelity DNA polymerase (NEB) using the entire 20 µL MaP-RT reaction and following manufacturer’s protocol with 20 cycles of amplification ...
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bioRxiv - Biochemistry 2024Quote: ... 1× Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs), 0.5 µM forward primer ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µM each DNAs were mixed in 1× T4 Ligase buffer (NEB, B0202S) and heated at 85°C for 10 min and cooled 5°C per 2 min down to 40°C ...
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bioRxiv - Genomics 2024Quote: ... and M-MuLV reverse transcriptase enzyme (200,000 U mL-1, New England Biolabs) was added per sample ...
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bioRxiv - Genomics 2024Quote: ... 1 μL T4-BGT (10U/μL) in a 50 μL reaction (NEB #0357) and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL T4 DNA ligase (400,000 cohesive end units/mL, New England Biolabs) and 4 µL 10× T4 DNA ligase buffer were added ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 μL Master Mix Golden Gate BsmbI or BsaI (New England Biolabs) for construction of Level 0 (L0 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM DTT) and incubated with 1 mg/mL streptavidin (New England Biolabs). The chamber was again washed with TIRF buffer and incubated with 10 μL of a fresh dilution of microtubules (1.5 μL microtubules diluted into 10 μL TIRF-Casein buffer [TIRF buffer supplemented with 50 mM KCl ...
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bioRxiv - Genetics 2024Quote: ... An additional 1 μL of T4 Ligase (200 U/μL; New England Biolabs) was then added ...
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bioRxiv - Neuroscience 2021Quote: ... 0.4 mM phenylmethylsulfonyl fluoride (PMSF) and 4 µM pepstatin) and digested with the chosen endoglycosidase (PNGase F, New England Biolabs; or Endoglycosidase H, Roche) in a small volume of the appropriate buffer ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of 0.2 mg/mL of streptavidin (NEB; N7021S) in crystallization buffer were added to the biotinylated lipid surface and incubated for 30 min in a humidity chamber at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... β1-4 galatosidase or α2-3,6,8 Neuraminidase (New England BioLabs) at concentration of 5000 ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of T4 DNA ligase (40U final, NEB M0202L), 1X T4 DNA ligase reaction buffer ...
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bioRxiv - Genomics 2021Quote: ... and 4 μl 5 U/μL I-SceI (NEB, #R0694L) in a 50 μL final volume for 3 hours at 37°C ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 4 μl 10× T4 RNA ligase buffer (New England Biolabs), 4 μl T4 RNA ligase ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 10x buffer 4 (New England Biolabs, NEB), 2 μL of 1 mg/ml bovine serum albumin solution (NEB) ...