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Citations for New England Biolabs :
1751 - 1800 of 1891 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Purified product was Gibson cloned into a previously described PiggyBac transposon vector (37) using standard protocols in a 10 μl reaction (NEB E2621S). 2.5 μl of the Gibson reaction was electroporated into 25 μl of 10-beta electrocompetent E ...
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bioRxiv - Cell Biology 2024Quote: ... Thymidine was then removed by washing cells with culture media and blocking of existing CENP-A was performed by treatment with 10 mM SNAP-Cell® Block BTP (S9106S, NEB) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... The mixture was then returned to ice for 5 minutes before 180 μL of NEB® 10-beta/Stable Outgrowth Medium (B9035, NEB) was added ...
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bioRxiv - Genomics 2023Quote: ... The pellet was resuspended in 90 µL of freshly prepared Micro-C “Master Mix 1” (10 μl 10x T4 DNA Ligase Buffer, 75 μl ddH2O, 5 μl T4 PNK (NEB #M0201L)) ...
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bioRxiv - Genomics 2023Quote: Restriction digestion was carried out by adding 25 μL of 10× CutSmart buffer and 100 U of the MluCI restriction enzyme (NEB #R0538) plus 100 U of the MboI enzyme (NEB #R0147) ...
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bioRxiv - Genomics 2023Quote: ... The reaction was diluted to 100 µL with 1X T4 PNK (polynucleotide kinase) buffer and incubated for 30 min with 1 µL T4 PNK (10 U/µL; NEB M0201) to remove 3’ phosphoryl groups ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng of RCA amplicons were treated with T7 endonuclease 5 μL of 10× reaction buffer and 1 μL of T7 endonuclease I (New England Biolabs, M0302S) in a 50 μL reaction volume ...
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bioRxiv - Systems Biology 2023Quote: ... The insert and the destination vector were ligated in a 10 µl reaction with 2X instant sticky-end ligase master mix (NEB #M0370S), using a 1:5 molar ratio of backbone-to-insert and otherwise following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were performed at 37°C for the indicated time (30 min if not otherwise specified) and then terminated by the addition of 10 μL of 2× RNA loading dye (New England Biolabs, USA). Reaction samples were heated at 85°C for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Nascent RNA was then fragmented at 85C for 10 min and converted to cDNA using NEBNext Ultra II RNA First Strand Synthesis Module (NEB, E7771) and NEBNext Ultra II Directional Second Strand Synthesis Kit (NEB ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from starter cultures of the three original ancestor isolates and 10 trimethoprim-resistant derivatives using New England Biolabs Monarch Genomic DNA Extraction Kit (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 μL of PCR product was added to a 10 μL reaction containing 0.2 μL DraI (New England BioLabs, MA, USA) and 1 μL rCutSmart™ Buffer (New England BioLabs ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL of 10 mM indexed P5 and P7 primer solutions and 25 µL NEBnext High-Fidelity 2X Master Mix (New England BioLabs: ME541L) were added ...
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bioRxiv - Biophysics 2022Quote: ... the reaction was diluted to 90 µl and was supplemented with 10 µl DNAse I buffer and 2 µl DNAse I enzyme (NEB #M0303S) and incubated for 15 minutes at 37□ C to degrade the DNA template ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Genomics 2022Quote: ... 10 U of the enzyme was used in the 50 μl-reaction containing 5 μl of rCutSmart Buffer (10X, NEB # B6004S) for 30 min-incubation at 37°C ...
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bioRxiv - Genomics 2022Quote: ... from ONTs ligation sequencing kit (ONT, SQK-LSK109) and 10 µl of NEBNext Quick T4 DNA Ligase (New England Biolabs, E6056S). Ligation mix was incubated at RT for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... was used as a template to prepare 10 μl cDNA using LunaScript®RT SuperMix Kit (New England Biolabs, cat# E3010L) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The sonicated DNA was end-repaired for 30 minutes at 20°C in 200 μl final volume containing 1x end-repair buffer and 10 μl of end-repair mix (NEB, E6050L). The reaction was cleaned in 2x AmpureXP Beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared with 10 ng of total RNA using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs). Single-end sequencing was performed on all the samples with a 75 bp sequencing depth on a NextSeq machine at the RNomics platform of the Université de Sherbrooke (Canada) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng of DNA was used for library preparation according to NEBNext® Ultra II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution of the libraries were estimated using Fragment Analyzer ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated for 2 h at 37°C in 100 μl 1x TdT buffer containing 4 μl 10 mM ATP and 1 μl Terminal Transferase (NEB M0315L). Plugs were rinsed with 1 ml Tris buffer (10 mM Tris HCl (pH 8.0)) ...
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bioRxiv - Biochemistry 2023Quote: ... was used to linearize plasmid (10 µg) by incubating at 37 °C for a minimum of 2 hours in 1x CutSmart buffer (NEB, B7204S). Linearized plasmid was purified by extraction with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... Small RNA libraries were then prepared from 10 ng of small RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs #E7300S) according to the manufacturer’s recommendation using NEBNext Multiplex Oligos for Illumina (Index Primer Set 1 ...
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bioRxiv - Genetics 2023Quote: ... cDNA and sequencing libraries were generated from 500 ng of fresh RNA samples with 10 cycles of PCR with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760). After quality checking using an Agilent 2100 Bioanalyzer ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Briefly 2 µM of dsDNA guide was ligated into 10 ng of pSp-Cas9-(BB)-2A-GFP using 400 units T4 DNA ligase (NEB M0202) and incubated for 16 hrs at 16°C ...
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bioRxiv - Microbiology 2023Quote: ... The cDNAs were diluted 1:10 and 2 μl of each used for subsequent PCR reactions with one unit of Taq polymerase (New England Biolabs, MA), 200uM dNTPs (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ddRAD library preparation included an initial digestion of 300 ng of DNA in a 34 μL reaction (2 hours at 37 °C; 10 U each of SbfI and MspI, New England Biolabs Inc.). Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes ...
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E. coli, NEB, Ref.C3020K) and grown overnight on LB plates supplemented with 50 μg/mL Carbenicillin (Carbenicillin disodium salt ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 µg of genomic DNA extracted using the Phenol-Chloroform method and treated with RNase A were digested with 10 units of DpnII (NEB, R0543S) in a final volume of 20 µL at 37°C for 6 hours ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Whole-cell extracts were quantified using the BCA assay and 10 µg was subjected to PNGase F (New England Biolabs, #P0705S) treatment according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The final ligation step was carried out for 10 min at room temperature using a 2X Quick Ligase Buffer (NEB; #B2200S) and a Quick Ligase (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... and then 5 μL of 10% NP-40 and 10X GlycoBuffer 2 as well as 1 μL of PNGase F (New England Biolabs #P0704S) were added to make a final reaction volume of 50 μL ...
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bioRxiv - Microbiology 2024Quote: ... 10 ng was subjected to an additional 10 cycles of amplification with Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs #M0494) using the same primers as the mutagenic PCR ...
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bioRxiv - Genetics 2024Quote: ... Digested vector fragments and insert DNA fragments were mixed in a volume of 10 μl containing 2U T5 exonuclease (New England Biolabs, M0363S), 12.5U Phusion DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: dsBC was cloned into the digested vector by combining 200ng vector (∼0.035pmol, 8uL), 10ng dsBC (∼0.175pmol, 2uL) and 10 μL 2x NEBuilder® HiFi DNA Assembly Master Mix (NEB cat#E2621L) and incubating at 50°C for 1hr ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Biophysics 2024Quote: ... We mixed the digested handles with the large 38 kb fragment in 10:1 molar ratio (biotin-handles to DNAparS) and added T4 DNA ligase (New England Biolabs, M0202L) and 1 mM ATP for ligation ...
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bioRxiv - Genomics 2024Quote: ... The no stem-loop plasmid library was digested for 8 hr at 55°C (10 μg plasmid library, 9 μL BsmBI (NEB R0739L), and 10 μL of Buffer 3.1 (NEB B6003S) ...
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bioRxiv - Biochemistry 2024Quote: ... the DNA digestion or cleavage activity of the proteins was performed by incubating 50 nM protein with 10 ng ml -1 pUC19 plasmid (NEB N3041S), in a 20 µl reaction at 37 °C for varying time conditions in 20 mM Hepes pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid pool was then digested with the outer guides M1 and P1 (5 μg plasmid pool, 10 μL M1+P1 Cas9 RNP, 4 μL 10X rCutSmart buffer (NEB, B6004S), and H2O to 40 μL incubated at 37°C for 1 hour) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the reaction was diluted to 90 µl and was supplemented with 10 µl DNAse I buffer and 2µl DNase I enzyme (NEB, cat. M0303S) and incubated for 15 min at 37 °C to degrade the DNA template ...
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bioRxiv - Developmental Biology 2024Quote: Cells at passage 35-60 were harvested as a single cell suspension using TrypLE Express and 800K cells were nucleofected in 100 μl final volume containing either 2 μg of the Cas9 nickase expression vector 47 with 1 μg of each of the two sgRNAs and 10 μg of the targeting vector (for the INS eGFP allele) or 6 μl Cas9-NLS protein (120 pmol, NEB, M0646M) with 2 μg of each of the sgRNAs (Table S2 ...
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bioRxiv - Systems Biology 2022Quote: ... of CROPseq-CaptureSeq-Guide-Puro backbone and 400 fmoles (18.31ng) of amplified dsDNA oligonucleotides were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...