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Citations for New England Biolabs :
1851 - 1891 of 1891 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Systems Biology 2022Quote: ... 100ng of vector backbone and 20ng of trcrRNA-CaptureSeq-5’LTR(truncated) cassette were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of total RNA from various tissues was used to construct RNA-seq library and sequence on the Illumina HiSeq X-10 platform following the protocol of NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Ipswich, MA, USA).
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bioRxiv - Systems Biology 2020Quote: ... reaction components were added for second strand synthesis (70 μL water, 20 μL NEB second strand buffer, 10 μL NEB second strand enzyme). Second strand synthesis was carried out as described and the double-stranded cDNA was used as input for tagmentation ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 µL of dA-tail reaction buffer and 10 µL of Klenow Fragment were added to the nuclei (both reagents were part of NEBNext dA-Tailing Module (NEB, #E6053L, Ispwich, MA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µ l of cfDNA was incubated at 37°C for 1 hour with the following reaction mixture: NEBuffer™ 2 (NEB, B7202), 0.25 mM MnCl2 (SIGMA ...
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bioRxiv - Microbiology 2022Quote: ... sequences of RcACE2 or hACE2 were PCR-amplified from pCAGGS-RcACE2- or pCAGGS-hACE2-expressing plasmids (10) and cloned into EcoRI- and XhoI-digested pCAGGS-blast plasmids using NEBuilder (New England Biolabs, Ipswich, MA, USA). Vero/TMPRSS2 cells were transfected with pCAGGS-blast-RcACE2 or pCAGGS-blast-hACE2 plasmids using the PEI MAX transfection reagent (Polysciences ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... The TD-PCR protocol began with an annealing temperature of Tm + 10 °C for the first cycling phase (Tm calculated by NEB Tm Calculator tool). The annealing temperature was then decreased by 1 °C per cycle until the primers’ Tm was reached ...
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bioRxiv - Molecular Biology 2020Quote: DNA adapters were prepared by resuspending complimentary oligos at a final concentration of 10 μM of each in CutSmart® buffer (New England Biolabs, Ipswich, USA). The reaction was then heated to 98°C for 2 minutes then set to ramp from 85°C to 65°C for 1 hour and finally from 65°C to 8°C for 30 minutes.
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bioRxiv - Biochemistry 2021Quote: ... 500 ng total of the purified PCR products were mixed with 1 μl 10× NEBuffer 2 (New England Biolabs Inc., Beverly, MA, USA) and ultrapure water to a final volume of 9.75 μl ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was analyzed in duplicate 10-µl reactions containing 5 µl Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA), 2 µM of each primer ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng of pLenti6/V5-DEST™ vector was used as template per reaction with Q5® High-Fidelity DNA Polymerase (NEB, #M0491S). The PCR program ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from each tail clip was standardized to 10 ng/µL and digested with the restriction endonuclease SbfI-HF (New England Biolabs, Ipswitch, MA, USA), and used to generate RAD-seq libraries33–35 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids cleavage was conducted at 37 °C for 10 min and stopped by addition of 2×RNA loading dye (New England Biolabs, Ipswich, MA, USA). Before loading ...
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bioRxiv - Zoology 2023Quote: Reverse transcription was conducted with 10 µL of eluted nucleic acids using the ProtoScript® II Reverse Transcriptase (New England Biolabs, Massachusetts, USA). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... and treated for 10 min at RT by RNase III enzyme (2 U of RNase III (Short Cut, New England BioLabs, Massachusetts, USA, M0245S) with 20 mM MnCl2 in 1× reaction ShortCut buffer) ...
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bioRxiv - Genomics 2024Quote: ... and half the elution was used as a template in a second round of 8-10 cycle PCR to prepare Illumina libraries using NEBNext multiplex oligos using the same conditions described above (NEB E7335L and E7500L). The final PCR product was purified using AMPure XP beads (Beckman Coulter A63882 ...
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bioRxiv - Genomics 2023Quote: ... and chromatin was digested during 10 min at 37 °C with 6 Kunitz units of MNase (New England Biolabs, 200 Kunitz units/µl) per 1 million cells ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... Washed Dynabeads containing the DNA-complexes were directly resuspended in 20 µL USER reaction buffer containing 10 µL StickTogether DNA Ligase Buffer 2x (NEB, cat. no. B0535S), 1.5 µL USER Enzyme (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no. N0552S; New England BioLabs, Ipswich, MA) and ran at 105 V for 45 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... An additional reaction was conducted where 10 µg was digested using 3 µl of EcoRI-HF and 3 µl of mung bean nuclease (New England Biolabs, catalog number: M0250) at 37 °C for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... Strand extension was performed at 37°C for 10 minutes using 5uM of ssDNA binding protein (T4 Gene 32 Protein, NEB, Cat No. M0300S) and 0.8U/µL of Klenow Fragment (3’-5’ exo- ...
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bioRxiv - Microbiology 2024Quote: ... 10^106 of DMSO or IMP-1088 released virions were treated with two-fold dilutions of Furin (New England Biolabs, Cat. no. P8077L) in 20 mM HEPES ...
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bioRxiv - Plant Biology 2024Quote: Cloning of the vectors pCAMBIA1300-AtSDR4Lpro::3xHA-AtSDR4L-3xFLAG and pCAMBIA1300-DIG2pro::3xHA-DIG2-3xFLAG was performed using Escherichia coli competent cells of NEB 10-beta strain (C3019H, NEB, Ipswich, MA, USA) and One Shot™ Mach1™ T1 strain (C862003 ...
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bioRxiv - Systems Biology 2024Quote: ... 50 µL reverse-crosslinking buffer (3.33 µL 0.5M EDTA, 1 µL 10% SDS, 2 µL Proteinase K NEB #P8111S, 43.67 µL EB buffer) was added to each sample ...
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bioRxiv - Molecular Biology 2020Quote: Individual LAMP assays were assembled in a total volume of 25 μl of 1X Isothermal buffer (NEB; 20 mM Tris-HCl, 10 mM (NH4)2SO4 ...
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bioRxiv - Genetics 2021Quote: ... The col-10p::TIR1 line was generated by cloning to combine the col-10 promoter with the TIR1 construct of pDD356 (NEB Hifi Assembly Master Mix). Plasmid pAP082 was used to express Cas9 and a sgRNA to target the chromosome I insertion site ...
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bioRxiv - Genetics 2024Quote: ... Cells were then pelleted by centrifugation at 500 x g for 10 min and washed with 200 µL of 1.5X DpnII reaction buffer (New England Biolabs Ipswich, MA cat no. R0543S), pelleted again and then resuspended in 300 µl of cold 1.5X DpnII reaction buffer (NEB cat no ...
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bioRxiv - Genomics 2024Quote: ... Samples were then rinsed with 2X SSC before incubating with a blocking buffer (10% BSA, 3% v/v 6% v/v murine RNase inhibitor [NEB, M0314L] in 2X SSC) for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 50 mL buffer 3 (20mM KPi pH 7.4, 1.2M Sorbitol, 10 mM ribonucleoside vanadyl complex (NEB S1402S, pre-warmed at 65 °C), 0.08mg/ml Zymolyase-20T (Amsbio 326921) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Python script by default selects codons with the highest usage frequency for the input organism (E. coli NEB 10-Beta in this study), unless specified problematic sequences are introduced (Supplementary Figure S3B) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were further lysed with probe sonication and the lysate was denatured by adding 10 µL of denaturing buffer (NEB PNGase F kit, P0709) and heating at 100 °C for 5 min ...
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bioRxiv - Genomics 2023Quote: ... BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Supplementary Table S7) in the following reaction mix: 10 µl 2X NEBNext® Ultra™ II Q5® Master Mix (NEB cat. M0544S), 1 µl 10 µM each bio-primer ...
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bioRxiv - Biochemistry 2024Quote: ... final concentration 30 nM) at 37 °C for 10 min before being added to M13mp18 single-stranded DNA (New England Biolabs, final concentration 25 ng/μL). Aliquots were quenched at the indicated time points by adding an equal volume of phenol-CHCl3-isoamyl alcohol and analyzed by agarose gel electrophoresis as described above.
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...