Labshake search
Citations for New England Biolabs :
1701 - 1750 of 10000+ citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2022Quote: ... 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB), 0.5 μL 10 mM dNTPs (NEB) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.5 μL Phusion polymerase and 10 μL 5X HF buffer (New England Biolabs, M0530L), supplemented with nuclease-free water to a total volume of 50 μL ...
-
bioRxiv - Microbiology 2023Quote: ... lysates (1 mg) were incubated with MNase (10 units/1 mg lysate; NEB #M0247S) at 37°C for 60 min ...
-
bioRxiv - Neuroscience 2023Quote: ... 10 µM of each primer was combined with OneTaq QuickLoad Master Mix (NEB #M0486) and run in a thermocycler as follows ...
-
bioRxiv - Cancer Biology 2023Quote: ... Streptavidin conjugated DNA was ligated to the appropriate concentration adapter (1:10) (NEB, 7335L). Then ...
-
bioRxiv - Molecular Biology 2024Quote: ... The eluent was mixed with 10 U of Bst 2.0 WarmStart DNA polymerase (NEB) and 1 × Q5 polymerase reaction buffer (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 mM β-ME) supplied with protease inhibitors and an RNase inhibitor (NEB, M0314L) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... 10 µL of Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, Massachusetts, USA), 0.5 µL of template DNA/cDNA ...
-
bioRxiv - Microbiology 2024Quote: ... Cells were harvested and resuspended with 1:10 volumes of CutSmart Buffer (NEB, # B7204) and 5 µl of 100 mg/ml RNase-A solution (pre-heated at 100°C for 20 minutes) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate TbGT8 5’_BSDr_TbGT8 3’_pUC19 ...
-
bioRxiv - Biochemistry 2024Quote: ... Peak fractions were further purified on 7.5-10 mL amylose columns (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
-
bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
-
bioRxiv - Genomics 2024Quote: ... 975 μL of pre-warmed 10-beta/Stable Outgrowth Medium was added (NEB #B9035) to each cuvette ...
-
bioRxiv - Evolutionary Biology 2021Quote: DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
-
bioRxiv - Genomics 2020Quote: NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext rRNA Depletion Kit (NEB, Ipswich, MA) was used to generate RNA-seq libraries from purified RNA following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: Libraries were constructed using NEBNext Ultra RNA Library Prep for Illumina kits (New England Biolabs kits E7490 and E7530), as per manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... a ChIP-seq library was made using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB).
-
bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
-
bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
-
bioRxiv - Genomics 2023Quote: ... Final immunoprecipitated DNA samples from the kit were then purified using a Monarch PCR and DNA cleanup kit (5μg) (NEB), with a final elution volume of 50μL in TE buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... libraries were quantified by qPCR using a library quantification kit (NEBNext® Library Quantification Kit, E7630, New England Biolabs). Illumina sequencing was performed on a HiSeq2500 instrument for 100 single end read cycles (Genome Sequencing Core ...
-
bioRxiv - Cell Biology 2019Quote: ... then incubated with either mouse anti-MBP antibodies at a dilution of 1:5000 (New England Biolabs) or 1:2500 mouse anti-GFP antibodies (Roche ...
-
bioRxiv - Cell Biology 2021Quote: ... The mouse C10 G54R mutation removes a cut site of the restriction endonuclease BanII (NEB, cat# R0119S), introducing a restriction fragment length polymorphism ...
-
bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
-
bioRxiv - Cancer Biology 2023Quote: Mouse NIK coding sequence in the pENTR shuttle plasmid was mutated using Phusion high fidelity polymerase (NEB) and oligonucleotides designed to carry the desired mutations (indicated by lowercase letters) ...
-
bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
-
bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
-
bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
-
bioRxiv - Genomics 2020Quote: ... Samples were pre-cleared by adding 40 µl Protein A Magnetic Beads (New England Biolabs, MA, United States) and rotating at 4°C for 60 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates containing 800μg of protein in a total of 400μl lysis buffer were pre-cleared by incubation with 15μl of Protein A magnetic beads (New England Biolabs) for 1h at 4°C under agitation ...
-
bioRxiv - Biochemistry 2020Quote: ... Cas9 RNPs were formed by incubating 1 pmol of sgRNA with 0.5 pmol of Cas9 protein in 1X NEBuffer™ 3.1 or 2.1 (NEB) at room temperature for 10 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... on ice for 30 min and protein concentration measured by BCA assay (7780, New England Biolabs, Beverly, USA). Results were analyzed using the Compass software (ProteinSimple) ...
-
bioRxiv - Microbiology 2020Quote: ... or CatL-cleaved GPs were incubated with Protein N– glycosidase F (PNGaseF, 250U; New England Biolabs, Ipswich, MA) under reducing conditions for 16 h at 37℃to remove N–linked glycans ...
-
bioRxiv - Molecular Biology 2021Quote: ... PARP1 and SET8 GST-fusion and mutant proteins were induced in Escherichia coli ER2566 cells (New England Biolabs
-
bioRxiv - Molecular Biology 2022Quote: ... Removal of sialic acids was performed using the α2-3,6,8 neuraminidase (New England Biolabs, 50 U/μg protein). Removal of fucose was performed using α1-2,4,5,6 fucosidase O (New England Biolabs ...
-
bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 40 μl of filtered double-distilled water and 20 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads ...
-
bioRxiv - Cell Biology 2019Quote: ... The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... σA-FLAG and RbpA-FLAG proteins were prepared by PCR using Q5® High-Fidelity DNA Polymerase (NEB) with primers #3130 + #3131 (HelD) ...
-
bioRxiv - Biochemistry 2021Quote: ... His tagged proteins were eluted with lysis buffer containing 400mM imidazole and bound immediately to amylose resin (NEB) for 1hr ...
-
bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
-
bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
-
bioRxiv - Molecular Biology 2021Quote: ... EcoRI sites underlined) and different concentration of recombinant His-SET8 protein (0 to 1.4 μM, New England Biolabs) were incubated for 10 min on ice in 1× GRB binding buffer [20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... Either 3-5 µL of Color Prestained Protein Standard-Broad Range (11-245 kDa) (New England Biolabs, P7712) or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa ...
-
bioRxiv - Cell Biology 2022Quote: ... residual ATP from the protein preparation was depleted using apyrase by mixing 4.5 µl of 500 nM protein with a 0.5 µl of apyrase (NEB), and incubating for 30 minutes at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: NSD1/2 WT and different cancer mutants (3.4 μM) were mixed with recombinant H3.1 protein (1 μg) (purchased from NEB) or recombinant H3.1 mononucleosomes in methylation buffer (50 mM Tris/HCl ...
-
bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
-
bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
-
bioRxiv - Biochemistry 2023Quote: ... and then deglycosylated and dephosphorylated overnight at 37 °C with the protein deglycosylation mix II (New England Biolabs) and Quick CIP (New England Biolabs) ...