Labshake search
Citations for New England Biolabs :
1701 - 1750 of 4951 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... then ligated to a custom 5′ adapter (10 μM oligocap) by T4 RNA Ligase I (M0437, New England Biolabs) with 1 μl RNaseOUT for 16 hours at 16°C ...
-
bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated and regions flanking Exon 5 were PCR-amplified using Q5 High-Fidelity DNA Polymerase (NEB) (oligonucleotide primers F ...
-
bioRxiv - Cancer Biology 2023Quote: ... were then denatured at 95°C for 5 min and cooled at room temperature to form heteroduplexes using NEBuffer2.1 1x (NEB). The T7 endonuclease I (NEB ...
-
bioRxiv - Microbiology 2023Quote: ... the 5’-PPP RNA in the total RNA was specifically capped with 3’-Desthiobiotin-GTP (New England BioLabs, N0761) by the Vaccinia Capping System (New England BioLabs ...
-
bioRxiv - Systems Biology 2023Quote: ... with 10 µL RT mix (5 µL 5x Maxima RT Buffer, 1.25 µL 10 mM/each dNTP [New England Biolabs #N0447S] ...
-
bioRxiv - Plant Biology 2024Quote: ... This end-repaired DNA was subjected to A-tailing using the Klenow 3′–5′ exo− enzyme (New England Biolabs). The subsequent step involved the ligation of methylated adapters to the A-tailed DNA ...
-
bioRxiv - Immunology 2023Quote: ... Total RNA samples (5-500 ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Cat# E7400; New England Biolabs) to diminish rRNA from the samples ...
-
bioRxiv - Biophysics 2023Quote: ... Reaction was terminated with 3 μL of stop buffer (5% sarkosyl, 0.025% bromophenol blue, 50% glycerol) and incubated with 1.6 units of Proteinase K (NEB, P8107S) for 15 minutes at 37 degrees before resolving on 1% agarose TAE gel stained with SybrSafe (Invitrogen ...
-
Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The quantitative PCR reaction mix contained 5 µl of the Luna Universal qPCR Master Mix (2X, New England BioLabs), 1 µl of the forward primer (10 µM) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Beads were subsequently resuspended in 20 μL dephosphorylation reaction mix (1X PNK buffer, pH 6.5, 5 units of T4 polynucleotide kinase (New England Biolabs), 0.5 μL RNaseOut Ribonuclease inhibitor (Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNase-treated RNA (10 µg) was fragmented by using 5 units of ShortCut RNase III (New England Biolabs, M0245) for 5 min at 37°C and the reaction was stopped by adding 10 µL nuclease free water ...
-
bioRxiv - Microbiology 2024Quote: ... Plasmid pEC095 was digested with BtsBI and the 5’ overhanging DNA ends were filled in with T4 Polymerase (NEB). A kanamycin resistance marker ...
-
bioRxiv - Genetics 2024Quote: ... DNA digested overnight with XhoI was further digested for ∼5 h with HF-BamHI or NgoMIV (New England Biolabs). Digested DNA was precipitated using standard ethanol/ sodium acetate precipitation.
-
bioRxiv - Microbiology 2024Quote: ... the protein eluted from MonoQ 5/50 GL was incubated with 10 μM SNAP-Surface Alexa Fluor 647 (NEB) for 1 hour at 4°C prior to gel filtration.
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was dephosphorylated by incubation with 5 U T4 Polynucleotide Kinase in 1X T4 PNK Buffer (New England BioLabs) supplemented with SUPERaseIn RNase Inhibitor for one hour at 37°C and ligated to bar-coded linkers (NI-810 ...
-
bioRxiv - Biochemistry 2021Quote: ... The fragments were ligated into the pLSV101 vector (1:1 and 3:1 molar ratios) with T4 DNA ligase (New England Biolabs) (10 °C for 30 s and 30 °C for 30 s alternating overnight) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 pM/μl TrAEL-seq adaptor 1 and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L). Plugs were then rinsed with 1 ml tris buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer, 1% triton X-100, and 100 U HpaII enzyme [NEB]) and incubated at 37 °C for 3 hours with constant shaking ...
-
bioRxiv - Genomics 2019Quote: ... the fragmented genomic DNA was ligated with 1 µL of 10 µM phosphorylated hairpin oligo mix (1 µL of NEB T4 ligase, 1 µL of 10x NEB T4 Ligase buffer ...
-
bioRxiv - Microbiology 2020Quote: ... using separate template dilutions (1:1 & 1:10) and high-fidelity Phusion polymerase (New England BioLabs Inc., Ipswich, MA, USA). A single round of PCR was performed using “fusion primers” (Illumina adaptors + indices + specific regions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proximity ligation was performed using 1 unit/ml RNA ligase 1 (NEB), 1x RNA ligase buffer ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The cells were resuspended in 1 mL of 1× NEBuffer 2.1 (NEB) and homogenized by grinding to a fine powder in liquid nitrogen ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 mM phenylmethylsulfonyl fluoride (PMSF) and 1 x Protease Inhibitor Cocktail (NEB) as described before (Sun et al. ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 μg of amilCP_Orange chromoprotein was digested with 1 μL PflMI (NEB) with r3.1 buffer (NEB ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 μl of 20,000 U.ml−1 exonuclease I (New England Biolabs M0293) and 5 μl of 10X exonuclease I buffer (New England Biolabs B0293S ...
-
bioRxiv - Biochemistry 2021Quote: ... 100 μM ATP including 1 unit/μL T4 RNA ligase 1 (NEB) (final volume 300 μL ...
-
bioRxiv - Microbiology 2023Quote: ... In vitro Xrn-1 digests were performed with 1U Xrn-1 (NEB), 10U RppH (NEB) ...
-
bioRxiv - Genomics 2022Quote: ... 1× CutSmart Buffer (NEB) and 15 units of Quick CIP (NEB ...
-
bioRxiv - Genomics 2022Quote: ... 1× CutSmart Buffer (NEB) in nuclease-free water to form RNPs ...
-
bioRxiv - Genomics 2020Quote: ... and NEBuffer 1 (NEB) to the library and incubated at 37 °C for 1 hour ...
-
bioRxiv - Biophysics 2019Quote: ... Apyrase (1 unit, NEB) was added and incubated overnight at 4° C ...
-
bioRxiv - Genetics 2021Quote: ... rSAP (1 Unit, NEB) and RNasin (20 units ...
-
bioRxiv - Genetics 2020Quote: ... 1:50 (NEB, 14001), (5 ...
-
bioRxiv - Genetics 2020Quote: ... 1:100 (NEB, 51255), (4 ...
-
bioRxiv - Molecular Biology 2022Quote: ... and ligase 1 (NEB) sequentially ...
-
bioRxiv - Microbiology 2021Quote: ... 1 mM ATP (NEB), 1×T4 DNA Ligase Buffer and 800 U T4 DNA ligase (NEB) ...
-
bioRxiv - Microbiology 2022Quote: ... 1× Buffer 2.1 (NEB), 2 µg BSA and 3 units of T4 DNA polymerase (NEB ...
-
bioRxiv - Biochemistry 2021Quote: ... coli Topoisomerase 1 (NEB), 0.1 mg/ml BSA ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 µl MlyI (NEB). The digest was incubated at 37°C for 1 hour ...
-
bioRxiv - Genomics 2021Quote: ... 1 mM NAD+ (NEB) and water to a volume of 20 μl ...
-
bioRxiv - Genomics 2022Quote: ... 1 mM dNTPs (NEB), 1 U/μL RNase Inhibitor (NxGen ...
-
bioRxiv - Molecular Biology 2023Quote: ... pyogenes (1 μl, NEB), and 0.2 µl phenol red were mixed in an Eppendorf tube (total volume 2.2 μl ...
-
bioRxiv - Genomics 2022Quote: ... 1 μl RNaseOut (NEB), 2 μl of 100 μM TSO ...
-
bioRxiv - Genomics 2023Quote: ... 1 mM ATP (NEB), 4 mM DTT (Promega) ...
-
bioRxiv - Microbiology 2023Quote: ... 1 µl DpnI (NEB) was added to the PCR mix ...
-
bioRxiv - Genomics 2023Quote: ... and 1× Thermopol (NEB) at 72°C for 60 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... after DNase 1 (NEB) treatment.
-
bioRxiv - Genomics 2024Quote: ... 1% BSA (NEB, B9000S), and 1U/ml Protector RNase Inhibitor (between 100-200 μl depending on pellet volume) ...
-
bioRxiv - Microbiology 2024Quote: ... 1 mM NAD+ (NEB), 4 µg of DNA or RNA oligo ...
-
bioRxiv - Plant Biology 2020Quote: ... APE1 gene with an additional 1500 bp on the 5’ side and 500 bp on the 3’ side was amplified by PCR using Q5 High-Fidelity DNA Polymerase (NEB) using primers 5’-TTATAATACCCACCCGTCAAAGCTGTG-3’ and 5’-TTATAACAGACTCATCCGGACCCCAA-3’ containing an additional PsiI restriction site (70°C ...