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Citations for New England Biolabs :
1651 - 1700 of 2641 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... sequences of all myc-his tagged ZFP36 proteins were digested using ClaI and PmeI restrictions enzymes (New England Biolabs), blunted using DNA polymerase I large Klenow fragment (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... and MYC-UBR7 and MBP-UBC8 proteins were purified using anti-MYC (Pierce) and anti-MBP (New England Biolabs) magnetic beads ...
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bioRxiv - Cell Biology 2024Quote: ... Total lysates (200 µg each) were incubated with or without 400 U lambda protein phosphatase (New England Biolabs, #P0753S) and 1 mM MnCl2 at 30 °C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... SNAP-fused proteins in the extract were then labeled by incubation with 0.4 μM SNAP-Surface-549 (New England Biolabs) for 1 hour at 4°C on a rotator ...
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bioRxiv - Genetics 2024Quote: ... chromatin was diluted with a lysis buffer up to 0.1-0.2% SDS and pre-cleared by incubation with Protein A magnetic beads (New England Biolabs, S1425S) for 2h at 4C with slow rotation ...
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bioRxiv - Cell Biology 2024Quote: ... were microinjected in the pronuclei with a mixture of 0.67 µM EnGen Cas9 protein (New England Biolabs, Cat.No. M0646T), two Cas9 guides RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted RNA was treated with 2% RNase-free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation.
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 2 nM HaloTag-JF549 and 5 nM SNAP-Cell® 647-SiR (NEB) before imaging.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... 7 μl PEG 8000 (17.5% final) and 1 μl of T4 RNA ligase 2 KQ (NEB, M0373L) in 1X T4 RNA ligase buffer ...
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bioRxiv - Microbiology 2021Quote: ... total RNAs were extracted from Caco-2 cells and followed by mRNA purification (Oligo dT beads, NEB). The mRNAs were fragmented and converted into double-stranded DNA by reverse transcription and then second-strand synthesis (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... 8 μL of the de-phosphorylated DNA was incubated with 2 μL T4 Polynucleotide Kinase (NEB M0203S), 3 μL γ32P-dATP (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified PCR products were denatured and reannealed in NEBuffer 2 (New England BioLabs, Ipswich, MA, USA), using a thermocycler ...
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bioRxiv - Neuroscience 2021Quote: ... 50mM Sodium Butyrate) and incubated in a 37C water bath with 2 microliters Micrococcal Nuclease enzyme (NEB) for eight minutes ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification products were evaluated in 2 % agarose with Low Molecular Weight DNA Ladder (New England BioLabs, Ipswich). Residual primers and nucleic acids were removed from PCR reactions with Exonuclease I (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions were performed with the Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs) with 100 ng of genomic DNA ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S, #E7500S), following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Microbiology 2020Quote: ... The reverse transcription reaction (20 μL) contained 2 μL 10x M-MulV buffer (B0253S, New England Biolabs), 1 μL of 50 μM Oligo 18 dT (SO132 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted RNA was treated with 2% RNase-free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2020Quote: cDNA was synthesized from 2 μg RNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB) per manufacture’s protocol ...
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DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7bioRxiv - Cell Biology 2020Quote: ... and ligated with oligos each containing a Cy5- or BHQ-2 label and NotI sticky ends (NEB) complimentary to the amplified DNA template ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs) and confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs), 10 μg RBD produced in CHO-S- ...
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bioRxiv - Molecular Biology 2022Quote: ... and the L3 DNA linker (Table S1) was ligated to RNA by T4 RNA Ligase 2 (NEB). The beads were washed with high salt buffer and PNK buffer ...
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bioRxiv - Biochemistry 2022Quote: ... mixing with 5 μL of stop buffer (50 mM EDTA and 2 mg/ml Proteinase K (NEB)) ...
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bioRxiv - Microbiology 2022Quote: ... 23 nt RNA (5’-GAAUCUAUACAUAAAGACCAGGC-3’) was capped with vaccinia capping enzyme and 2’-O-methyltransferase (NEB) and radiolabelled with [α 32P]-GTP ...
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bioRxiv - Biochemistry 2022Quote: 2 mg of TconTS1 were incubated with 40,000 Units EndoHf (New England Biolabs, Frankfurt am Main, Germany) for 16 h at 37°C in 2.0 mL 10 mM phosphate buffer pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... DNA from 2 biological replicates was purified with Monarch PCR & DNA Cleanup Kit (Cat. no. T1030, NEB). Library preparation ...
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bioRxiv - Microbiology 2020Quote: ... and 2 μL of endo-β-N-acetylglucosaminidase-H (Endo-H, EC 3.2.1.96) (New England Biolabs™) and incubated in the thermomixer at 37 °C for one h ...
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bioRxiv - Microbiology 2021Quote: ... The oligos were then ligated into the double digested psiCheck-2 plasmid using T4 DNA ligase (NEB) at room temperature for 2 h ...
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bioRxiv - Immunology 2022Quote: ... GTP was added to a final concentration of 2 mM along with T4 RNA ligase buffer (NEB). RNA was then heated at 55°C for 8 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... SARS-CoV-2 S was amplified by PCR (Q5 High-Fidelity 2X Master Mix, New England Biolabs) from pUC57-nCoV-S (kind gift from Jonathan Abraham lab) ...
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bioRxiv - Systems Biology 2020Quote: SARS-CoV-2 S was amplified by PCR (Q5 High-Fidelity 2X Master Mix, New England Biolabs) from pUC57-nCoV-S (gift of Jonathan Abraham) ...
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bioRxiv - Genetics 2020Quote: ... genomic DNAs from AAV9-sgRNA infected SCs were amplified using Q5 High-Fidelity 2× Master Mix (NEB). PCR products were purified through QIAQuick PCR purification kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... The mixture was cooled to room temperature and 2 μL of 20 μM Cas9 (NEB, Ipswich, USA) was added (final concentration of 10 μM of Cas9 and 25 μM of gRNA) ...
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bioRxiv - Genomics 2020Quote: ... were assembled by hybridization and ligation over a complementary DNA strand using T4 RNA ligase 2 (NEB). To construct the hairpins ...
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bioRxiv - Genetics 2020Quote: ... corresponding to the point mutation in the SARS-CoV-2 genome (Q5 Site-directed mutagenesis kit, NEB). Site directed mutagenesis primers (Table S1 ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 µl of 10x Promega T4 ligase buffer (contains ATP) with 1µl of PNK kinase (NEB) and incubated at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... was ligated to the RNA fragments on bead using T4 RNA ligase 2 truncated KQ (NEB M0373) at 16°C ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged and resuspended in 600 μl Blocking buffer (PBS + 2 % BSA (B9000S, New England Biolabs)) and incubated for 5 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext Ultra 2 DNA Library Preparation Kit (New England Biolabs, MA, USA) with AMPure® XP Beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... The beads were drained of all liquids before 2 mg/ml of Proteinase K (New England Biolabs) diluted in PK buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The pLVX-EF1alpha-SARS-CoV-2-Nsp1/Nsp2-2XStrep-IRES-Puro plasmids were digested with BamHI (NEB) and MluI (NEB ...
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bioRxiv - Immunology 2020Quote: ... (2) and (3) was synthesized through HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs). All the mRNA products were concentrated and purified via EZ-10 Spin Column RNA Cleanup & Concentration Kit (Bio Basic Inc.) ...
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bioRxiv - Genomics 2021Quote: ... and washed several times before being resuspended the beads in 20 µl NEBuffer 2 (New England Biolabs). The final library was generated using the Illumina TruSeq kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: 3’ linker ligation (1x PNK buffer, 800 U T4 RNA ligase 2 truncated KQ (NEB, Cat#M0373L), 80 U RNaseOUT ...
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bioRxiv - Cancer Biology 2022Quote: ... Fragments were end-modified with adaptors using NEBNext Multiplex Oligos for Illumina Set 2 (New England BioLabs), following library amplification (PCR ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... were ligated to 3′ barcoded DNA adapters using truncated T4 RNA ligase 2 (New England Biolabs, #M0373). These fragments were separated in an 12% denaturing polyacrylamide-urea gel ...
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bioRxiv - Molecular Biology 2024Quote: ... After CIP inactivation (2 min at 80°C) piRNAs were 5΄end radiolabeled by T4 PNK (NEB) with [γ-32P] ATP (10mCi/mL ...