Labshake search
Citations for New England Biolabs :
1651 - 1700 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
-
bioRxiv - Neuroscience 2024Quote: ... An imaging activation mix was prepared by adding RNAse inhibitor (100 μL, New England BioLabs) to 250 μL Imaging Buffer Activator (VIZGEN ...
-
bioRxiv - Molecular Biology 2023Quote: ... 100 μl of a master mix that contained 1X DNA Adenylation Buffer (New England Biolabs), 100 μM ATP ...
-
bioRxiv - Molecular Biology 2023Quote: ... Diluted cDNA (100-fold) was used for two multiplex PCR reactions with Q5 polymerase (NEB) with two primer pools (Table S5) ...
-
bioRxiv - Plant Biology 2023Quote: ... For size comparison a radioactively labelled DNA ladder (100 bp DNA ladder, New England Biolabs) was applied.
-
bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Crosslinked nuclei were first resuspended in 100 µl 1x r2.1 restriction buffer (New England BioLabs) and then treated with 2% SDS for 1h at 37°C and with 1% Triton X-100 for 1h at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... treating each well with 100 μl of lysis buffer (0.45% NP40, 0.45% Tween20, 0.5x NEB Q5 PCR buffer ...
-
bioRxiv - Developmental Biology 2023Quote: ... 7 μL of 2x NEBNext Library Quant Master Mix with 1:100 low ROX (NEB), and 1.5 μL ddH2O ...
-
bioRxiv - Plant Biology 2023Quote: ... 100 µg of dual crosslinked enriched chloroplasts were resuspended in 1X DpnII reaction buffer (NEB) and digested with 20 U DpnII at 37°C overnight with gentle shaking ...
-
bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 25°C in 100 μl 1x T4 RNA ligase buffer (NEB) containing 40 μl 50% PEG 8000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 100 μM forward and reverse oligo pairs were combined with T4 polynucleotide kinase (PNK) (NEB) and 10X T4 ligation buffer (NEB ...
-
bioRxiv - Developmental Biology 2024Quote: 2 μg of genomic DNA was digested with 100 units of Taq1-v2 (NEB R0149S) and 100 μg of RNaseA overnight ...
-
bioRxiv - Developmental Biology 2024Quote: ... dissociated cells were ethanol-fixed and incubated with 1:100 anti-H3K27me3 antibody (NEB, 9733S) as described previously (34) ...
-
bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
-
bioRxiv - Biochemistry 2024Quote: ... The vector was linearized by 100-fold overdigestion by NdeI and XhoI (New England Biolabs) and then purified by gel extraction (Qiagen) ...
-
bioRxiv - Microbiology 2024Quote: ... Peaks were identified by comparison to a standard 100 bp dsDNA ladder (New England Biolabs) that were fit to an exponential function of molecular weight versus distance.
-
bioRxiv - Biophysics 2024Quote: ... 100 μg of each RBD was treated with endo F3 (purchased from New England Biolabs) in 1x Glycobuffer (50 mM sodium acetate ...
-
bioRxiv - Genomics 2024Quote: ... 100-500 ng of genomic DNA was fragmented using 2 units of Nt.CviPII (NEB # R0626S) in 500 µl of 1X NEBuffer 2 at 37°C for 4 hrs to overnight ...
-
bioRxiv - Biochemistry 2024Quote: ... The molecular weight standard used was the Quick-Load Purple 100 bp DNA Ladder (NEB). The PCR product band corresponding to the expected molecular weight of the RT-PCR product of the intron reverse spliced into the plasmid was excised from the gel ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... we digested 100 ng of DNA with the endonuclease PstI-HF (NEB Inc., MA, USA). Fragments were ligated to adaptors including the Illumina sequencing adaptor and individual barcodes with T4 ligase (NEB Inc. ...
-
bioRxiv - Bioengineering 2024Quote: ... media containing 100 μg/mL ampicillin and 109 p.f.u./mL of M13K07 helper phage (NEB) was added for overnight phage amplification in 37°C ...
-
bioRxiv - Genomics 2024Quote: ... chromatin was digested overnight with HindIII enzyme (100 U/μl, New England Biolabs, Ref. #R0104T). Then ...
-
bioRxiv - Synthetic Biology 2024Quote: ... using reaction volumes of 100 µL with 200 µM of dNTPs (New England Biolabs, N0447S). Reactions were prepared on ice in a PCR hood before being transferred to a preheated thermocycler (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... 1kb and 100 base pair DNA ladder Quick Load® markers (NEB # N0468L and N0467L) and 6x Gel loading Dye (NEB #B7024S ...
-
bioRxiv - Bioengineering 2024Quote: The Cas12a-crRNA complex was created by combining 100 nM LbCas12a (M0653T, New England Biolabs) and 100 nM crRNA (Integrated DNA Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... RNase H treatment on fixed cells was performed using 5 U RNase H (M0297, NEB) for 3 h at 37°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo-) (NEB M0212S) and ligation with Illumina sequencing adapters using T4 DNA ligase (NEB) ...
-
bioRxiv - Molecular Biology 2021Quote: ... a set of 5’-end biotinylated anti-sense DNA oligoes and 5ul RNase inhibitor (NEB) were added to the lysate ...
-
bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides were 5-end labeled with 32P-γATP using T4 polynucleotide kinase (New England Biolabs). Supercoiled pUC19 plasmid dsDNA was prepared by a method that did not involve DNA denaturation (Clewell and Helinski ...
-
bioRxiv - Genomics 2020Quote: ... A-tailing 3’ end was performed using Klenow Fragment (3’→5’ exo-) (New England Biolabs), and then TruSeq Adapters were ligated by Quick T4 DNA Ligase (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2021Quote: 5’ RACE was performed using the Template Switching RT Enzyme Mix (New England Biolabs M0466) according to manufacturer’s instruction ...
-
bioRxiv - Genomics 2021Quote: Decapping of 5 µg total RNA was performed with 200 units of yDcpS (NEB M0463S) in 10mM Bis-Tris-HCl pH 6.5 ...
-
bioRxiv - Genomics 2021Quote: ... The ligation mix (5 μL of LNB, 3 μL of Quick ligase (New England Biolabs), 1.2 μL of MQ ...
-
bioRxiv - Developmental Biology 2020Quote: ... followed by addition of 3’-A overhangs using Klenow Fragment 3’-5’ exo- (NEB, M0212S). After denaturation of DNA at 95°C for 3 min ...
-
bioRxiv - Genomics 2020Quote: ... 5 μL of Phusion High Fidelity 2× Master mix (New England Biolabs, Beverly MA, USA) and 2 μL of 10 μM standard Illumina P1 and P2 primers ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
-
bioRxiv - Pathology 2021Quote: ... The second strand was synthesized with Klenow Fragments (3′-5′ exo-; New England Biolabs Inc.) and complement chains of NSR primers ...
-
bioRxiv - Cell Biology 2021Quote: ... purified HEK-proGATI (∼5 μg) was denatured and deglycosylated using deglycosylation mix II (NEB, #P6044S), which contains the enzymes needed to remove N-linked and many common O-linked glycans ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... samples were digested for 15 min at 37 °C with 5 μL of ExoI (NEB) to remove first round primers ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
-
bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
-
bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
-
bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
-
bioRxiv - Biochemistry 2020Quote: ... The 5’ phosphate of transcribed RNA ligands was removed using calf intestinal phosphatase (CIP, NEB) and then replaced with 32P using T4 polynucleotide kinase (PNK ...
-
bioRxiv - Microbiology 2020Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs; 0.01 units/μL), Taq DNA ligase (New England BioLabs ...
-
bioRxiv - Genomics 2022Quote: ... We performed a second bead binding followed by a 5’ decapping with RppH (NEB, M0356S). RNA was phosphorylated on the 5’ end using T4 polynucleotide kinase (NEB ...
-
bioRxiv - Genomics 2022Quote: ... 5 μl of highly concentrated HhaI enzyme (150,000 U/mL, NEB; Cat. Num. R0139B-HC1), while keeping the remaining reagents as stated in the Tapestri protocol (5 μl Forward Primer Pool ...
-
bioRxiv - Cell Biology 2021Quote: ... A-tailing 3’ end was performed using Klenow Fragment (3’→5’ exo-) (New England Biolabs), and then TruSeq Adapters were ligated by Quick T4 DNA Ligase (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2020Quote: ... containing 0.1 mM dATP and 15 units of Klenow Fragment (3’→5’ exo-) (NEB, M0212M) and incubated at 37°C for 30 min ...