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Citations for New England Biolabs :
1501 - 1550 of 3130 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and 72°C for 5 min) (Phusion High-Fidelity DNA Polymerase, NEB, M0530S) using the P7 and a T7-fused P5 primer (5′ TAA TAC GAC TCA CTA TAG GGA ATG ATA CGG CGA CCA CCG A 3′ ...
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bioRxiv - Biochemistry 2023Quote: ... NEB Stable (lentiviral vectors) and NEB 5-alpha (other plasmids) (New England Biolabs) were used as cloning strains ...
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bioRxiv - Plant Biology 2023Quote: ... treated with 5 µg/mL Proteinase K (New England Biolabs Inc., MA, US) for 5 mins ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Genomics 2023Quote: ... and 5 μL of 300,000 U/mL MNase (New England Biolabs, CAT #M0247S). Efforts were made to avoid freeze-thaw cycles of the MNase enzyme ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was phosphorylated at the 5’ end by T4 polynucleotide kinase (NEB) and ligated to the 5’ adapter (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ ends were hydroxy-repaired by adding T4 polynucleotide kinase reaction mix (NEB) which was incubated for 45 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB) and checked on 1% agarose gel and approximately 600 ng of each PCR product was used as template for in vitro dsRNA synthesis according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2 and 2 U of recombinant Escherichia coli RNase HI (NEB) were added ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were mixed with Hydrophilic Streptavidin Magnetic Beads (5 µl) (NEB #S1421S) and binding buffer (500 µl ...
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bioRxiv - Biochemistry 2024Quote: ... and then ligated to cDNA using Thermostable 5’ App DNA/RNA Ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... PM187B20 was 5’ end labelled using 1 μL T4 polynucleotide Kinase (NEB, M0201S), 1 μL γ 32P ATP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 and 5 minutes by the addition of 0.8 units Proteinase K (NEB). Fragments were resolved on 1% agarose gel and band intensities were quantified on Image Lab (BioRad).
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bioRxiv - Molecular Biology 2024Quote: ... and 5 mM BME) onto a 10 ml amylose column (New England Biolabs). The amylose column was washed with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was degraded with 5 units of each RNase H (BioLabs cat #M029L) and RNase A/T1 (Thermo Scientific cat #EN0551) ...
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bioRxiv - Cancer Biology 2024Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Bioengineering 2024Quote: ... phosphorylated at the 5’ ends with T4 polynucleotide kinase (New England Biolabs M0201), annealed to generate a double-stranded insert ...
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bioRxiv - Genomics 2024Quote: ... 5 units of PolyA polymerase (E. coli or yeast as appropriate) (NEB#M0276S). The reaction was incubated at 37°C for either 7 or 30 minutes and stopped by purification with a MEGAclear column (Termofisher AM1908 ...
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bioRxiv - Cancer Biology 2024Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Cancer Biology 2024Quote: ... The initial 5 cycles of PCR were performed using Q5 polymerase (NEB M0491), 200uM dNTPs (Invitrogen 10297-018 ...
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bioRxiv - Bioengineering 2024Quote: ... Tagmentation reactions were halted using 5 µL of a 50% proteinase K (NEB) solution (mixed with H2O ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.5 μl Klenow fragment (3′ to 5′ exo-, Cat. #M0212L; New England Biolabs) and 0.5 μl T4 Polynucleotide Kinase (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... Tagmentation reactions were halted using 5 µL of a 50% proteinase K (NEB) solution (mixed with H2O ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Genetics 2022Quote: ... with insert-specific primers LC186 and 187) between hlh-30 and 3XFLAG-tag segments with HiFi cloning (NEBuilder® HiFi DNA Assembly Cloning Kit, New England BioLabs Inc., Ipswich, MA). Lastly ...
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bioRxiv - Neuroscience 2022Quote: ... The lysate was kept on ice for 30 min followed by the addition of RNAse If (125 U/ml; NEB, Ipswich, MA; Cat#M0243L) and was allowed to incubate in a thermomixer set to 1200 RPM at 37°C for 3 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CA using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, #E7775) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs) from 1 µg total RNA and Illumina sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... using an RNA input of 100 ng per sample (New England BioLabs, Ipswich, USA). Subsequently ...
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bioRxiv - Genetics 2021Quote: ... After digesting 100 ng of DNA from each sample using EcoRI and MseI (NEB), adapters containing barcode sequences were ligated to the ends of the DNA fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... chromatin fraction was solubilized in Buffer A with 1:100 micrococcal nuclease enzyme (NEB) and incubated for 15 min at 37°C shaker.
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bioRxiv - Molecular Biology 2020Quote: Tailing and ligation: Plugs were equilibrated once in 100 μl 1x TdT buffer (NEB) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and 100 ng of the sgRNA in NEBuffer 3.1 (New England Biolabs, Ipswich, USA) for 20 minutes at 37°C and 10 minutes at 65°C ...
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bioRxiv - Cell Biology 2021Quote: ... pH 7.8) and 100 μg/ml of proteinase K (New England Biolabs, Ipswich, MA) for 15 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... H2O up to 100 μL) was performed with phi29 DNA polymerase (New England Biolabs) at 30 °C for 20 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 100 ng of genomic DNA was treated with McrBC enzyme (New England Biolabs, M0272L) at 37°C overnight and then for 10 min at 75°C to deactivate the enzyme ...
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bioRxiv - Plant Biology 2021Quote: ... GST was cleaved using TEV protease (100 μL at 400 μM – New England Biolabs), and GST beads were added to eliminate GST and undigested proteins ...
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bioRxiv - Biochemistry 2021Quote: ... Extra RT primers were removed by Exonuclease I (100 U; M0293S, NEB, Ipswich, MA) for 2 hr at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... proximity ligated sample was treated with 100 μL Proteinase K (NEB P8107S-800U/ml), 100μL 10% SDS (Invitrogen Cat no ...
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bioRxiv - Genetics 2020Quote: ... 60 µg of this 3C-extract were digested with 100 units NcoI-HF (NEB) over night ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was digested overnight (ON) with either 2 μl (100 U) Dpn II (NEB) (later experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of isolated DNA was digested with MspI restriction enzyme (NEB, Ipswich, MA), carried out end-repair/adenylation (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... ∼100 ng of RNA was treated with NEBNext rRNA Depletion Kit (New England Biolabs). RNA-Seq was performed using libraries prepared with NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: 100 μM of compound was incubated with 0.02 units P1 nuclease (New England Biolabs) in P1 reaction buffer (50 mM sodium acetate pH 5.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 µg of DNA was incubated with 0.1 U/µL of USER enzyme (NEB) in 500 µL at 37°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... the DNA was washed once with 100 μl of 1x λ exonuclease buffer (NEB). The DNA bound to the beads was made ss by λ exonuclease digestion of the strand with 5’ phosphate (5 U/mg DNA ...
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bioRxiv - Biophysics 2024Quote: ... 100 ng or less template DNA and 24 μL Taq DNA polymerase (NEB M0273L). Purify and concentrate the PCR DNA product by ethanol precipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... were annealed (81 µl of each Oligo 100 µM, 1x T4 ligase buffer; NEB) at 98 °C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... were annealed (81 µl of each Oligo 100 µM, 1x T4 ligase buffer; NEB) at 98 °C for 5 min ...