Labshake search
Citations for New England Biolabs :
1651 - 1700 of 2109 citations for 6 Benzofurancarboxamide N 2 azabicyclo 2.2.1 hept 6 yl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... Pmex-5::PH-GFP-cyk-1(700-1437)::tbb-2 3’UTR in the pCFJ150 backbone [89] was made using HiFi cloning (New England Biolabs). Notably ...
-
bioRxiv - Immunology 2021Quote: ... pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). 293T cells were transfected with 3.75 μg pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G ...
-
bioRxiv - Immunology 2021Quote: ... Two restriction sites NheI at the 5’ end and BamHI at the 3’ end were incorporated into the CD4-polypeptide linker plasmid which was then inserted into pACP-tag(m)-2 plasmid (New England Biolabs) to obtain pACP-CD4 ...
-
bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 S R403T and RaTG13 S T403R/T403A mutant plasmids were generated using Q5 Site-Directed Mutagenesis Kit (NEB).
-
bioRxiv - Plant Biology 2021Quote: GFP coding sequence was amplified from plasmid p2GWF7 (Karimi et al., 2002) using GFPF40 and GFPR40 primers (see Supplemental Method 2) with Phusion polymerase (New England BioLabs) according to the provider’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were fully linearized by incubation at 37°C for a minimum of 2 hours using ClaI restriction enzyme (New England Biolabs). Full-length genomic RNA was transcribed from the linearized plasmids using the MegaScript T7 Kit (Ambion) ...
-
bioRxiv - Biochemistry 2020Quote: ... 20 µl of 2 U/µl Phusion polymerase and 160 µl of 40 U/µl Taq ligase (all enzymes from NEB). ddH2O was then added to a final volume of 1.2 ml.
-
bioRxiv - Biophysics 2021Quote: SARS-CoV-2 S N501Y plasmid was obtained from SARS-CoV-2 S plasmid (HDM-IDTSpike-fixK) by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). SARS-CoV-2 S and SARS_CoV-2 S N501Y pseudotyped retroviral particles were produced in HEK293T cells as described previously (29) ...
-
bioRxiv - Biophysics 2020Quote: ... The construct was amplified by PCR and inserted into pSNAP-tag®(T7)-2 vector (New England Biolabs Inc. #N9181S) with a SNAPf-EGFP-6His cassette ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
-
bioRxiv - Synthetic Biology 2022Quote: ... αlvus tRNAPyl (Ma-tRNAPyl)35 was prepared by annealing and extending the ssDNA oligonucleotides Ma-PylT-F and Ma-PylT-R (2 mM, Supplementary Table 1) using OneTaq 2x Master Mix (NEB). The annealing and extension used the following protocol on a thermocycler (BioRad C1000 Touch™) ...
-
bioRxiv - Developmental Biology 2022Quote: ... PE3-EGFP-2-Fw and PE3-EGFP-2-Rv) were annealed and inserted into the BsmBI sites of BPK1520 using a Quick Ligation Kit (New England BioLabs). PuroR cDNA was amplified from PX459 with primers (PuroR-Fw ...
-
bioRxiv - Biochemistry 2022Quote: ... His-MBP-tagged proteins were first purified by Ni-NTA affinity chromatography as above and then applied for 2 h to an amylose agarose matrix (NEB). Bound proteins were eluted with wash buffer containing 20 mM maltose ...
-
bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 2.5 ng of the input RNA was subjected to 35 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
-
bioRxiv - Molecular Biology 2022Quote: ... and mixed with a master mix consisting of 20 μl of 2 x NEBNext Ultra II Q5 Master Mix (NEB), 1 μl of 40 μM P5* primer ...
-
bioRxiv - Molecular Biology 2022Quote: ... and a volume of cDNA corresponding to 5 ng of the input RNA was subjected to 30 cycles of PCR with the 2 x NEBNext Ultra II Q5 Master Mix (NEB).
-
bioRxiv - Biochemistry 2022Quote: ... D-loops were deproteinized by adding 2 μL of 5% lithium dodecyl sulfate and 1 μL of 20 mg/mL proteinase K (New England Biolabs), and incubating the mixture at 37°C for 15 min ...
-
bioRxiv - Biochemistry 2022Quote: ... To seventeen microliters of the eluates were added 2 μL of 10x Nucleoside Digestion Mix reaction buffer and 1 μL of Nucleoside Digestion Mix (New England Biolabs). Reactions were incubated at 37 °C for 1 hour ...
-
bioRxiv - Microbiology 2022Quote: ... Nsp1 and 2 genes were cloned into pENTR (L1-L2) using Hifi DNA Assembly (New England Biolabs, Ipswich, MA, USA). LR recombination Gateway (ThermoFisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: SNAP-tag labeling of SNAP-mGluR2 was done by incubating cells with 2 µM of SNAP-Surface Alexa Fluor 549 (NEB) and 2 µM of SNAP-Surface Alexa Fluor 647 (NEB ...
-
bioRxiv - Biophysics 2022Quote: ... The agarose was digested by 1 hour incubation at 42 °C with 2 units of beta-agarase (M0392, New England Biolabs). After this stage ...
-
bioRxiv - Biochemistry 2022Quote: ... and cloned in the EcoRV and BamHI restriction site of the pBBR1MCS-2 (55) using the NEBuilder Assembly kit (New England BioLabs). pBBR1MCS-2 was a gift from Kenneth Peterson (Addgene plasmid # 85168 ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA chimera was then cloned into the pACP-tag (m)-2 vector (addgene# 101126) using NheI and NotI (NEB) as the restriction sites ...
-
bioRxiv - Developmental Biology 2020Quote: ... The supernatant was removed using a magnetic separator and washed once with 1 mL NEBuffer 2 and resuspended in 115 mL of Ligation reaction with Quick Ligase buffer (NEB), 6,000 U of Quick Ligase (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... the V1-V3 region of 16S rRNA genes was amplified from 2 ng of DNA in 50 μl reactions containing the following components: 1x Standard Taq Reaction Buffer (NEB), 3 mM MgCl2 (NEB) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and the NIR fluorescent adaptor (5′-OH-AGATCGGAAGAGCGGTTCAGAAAAAAAAAAAA/iAzid eN/AAAAAAAAAAAA/3Bio/-3′) was ligated to the RNA using truncated RNA ligase 2 K227Q (NEB) overnight at 16°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The ends of DNA were repaired by incubating in 70 μL of 1X NEBuffer 2 containing 0.6 units of T4 DNA polymerase (NEB, M0203L), 2 units of T4 polynucleotide kinase (NEB ...
-
bioRxiv - Molecular Biology 2020Quote: ... Beads containing the repaired DNA were resuspended in 50 μL of 1X NEBuffer 2 containing 0.1 mM dATP and 25 units of Klenow Fragment (3’→5’ exo-) (NEB, M0212M), and incubated at 37°C for 30 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were ligated to barcode primers 1-20 of NEBNext Index Primers for Illumina sets 1 and 2 (New England Biolabs) and libraries analysed using DNA High Sensitivity chip on an Agilent 2100 Bioanalyzer before being pooled ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl 10 pM/μl TrAEL-seq adaptor 1 and 1 μl T4 RNA ligase 2 truncated KQ (NEB M0373L). Plugs were then rinsed with 1 ml tris buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... The new SNAP-tagged histones synthesized during the chase were fluorescently labelled with 2 µM of the red-fluorescent reagent SNAP-cell TMR star or SiR-647 (New England Biolabs) during a 15 min-pulse step followed by 30 min wash in fresh medium ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μg of recombinant PbChiB2 and PbChiB4 resuspended in 20 mM Tris (pH 8.0) were independently mixed with 2 mg of chitin magnetic beads (NEB, CAD), chitin from shrimp shell (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... double-strand DNA repair templates were amplified with PCR from the plasmids pSL779 for gfp or pSL780 for mKate2 using Phusion polymerase and the primers listed in table 2 (New England Biolabs) (Bone ...
-
bioRxiv - Microbiology 2021Quote: ... Non-encapsidated nucleic acids were removed by mixing 900 μL of each sample with 100 μL 10x DNase buffer and supplemented with 2 μL (4 U) of DNase I (NEB) and 1 U of RNase ...
-
bioRxiv - Immunology 2020Quote: ... the DNA ladder (Quick-Load® Purple 2-Log DNA ladder 0.1-10 kb, #N0550S) were provided by New England Biolabs (NEB), The polymerase enzyme (Accustart Taq DNA polymerase HiFi ...
-
bioRxiv - Biochemistry 2020Quote: pTXB1 Halo 3C SARS-CoV-2 (2019-nCoV) Nsp1 1-179-Intein CBD (DU67780 was made using NEBuilder (New England Biolabs), amplifying the vector from existing clone DU28033 (pTXB1-HALO-Mxe Intein-CBD ...
-
bioRxiv - Biochemistry 2020Quote: ... NC-siRNA sense: 5’-AGGUAGUGUAAUCGCCUUGdTdT-3’.35,36 NEBuffer™ 2 and nucleoside digestion mix were obtained from NEB (Ipswich, MA). Adenosine and N6-methyl adenosine (m6A ...
-
bioRxiv - Molecular Biology 2022Quote: ... These 2 fragments were assembled into pCOM1 (Hayashi et al., 2016) at Van91I sites by Gibson assembly (New England Biolabs). The assembled plasmid ...
-
bioRxiv - Immunology 2022Quote: ... 2018” tagmentation mix were either sorted into plates containing RCB buffer for condition “hiSDSprotK-TWEEN” (2 x RCB: 100 mM Tris-HCl pH 8, 100 mM NaCl, 40 µg/mL Proteinase K (NEB), 0.4 % SDS (Sigma ...
-
bioRxiv - Cell Biology 2022Quote: ... 100 μl of MBP-SYCP1N at 2 mg/ml was incubated for 20 minutes on ice with 100 ul amylose resin slurry (NEB) equilibrated in 20 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... and inserted into the pSB_Avi_3×FLAG_insert_mScarlet_Puro vector (linearized by AsiSI and NotI) using Gibson assembly based on protocol instructions of 2×HiFi master mix (New England Biolabs). The correct constructs were selected based on Sanger Sequencing results (Eurofins).
-
bioRxiv - Synthetic Biology 2019Quote: 2 μL of the Dynabeads were used as template for the final PCR using barcoded P5 and P7 primers and Q5 HiFi 2× Master Mix (NEB) + SYBR Green ...
-
bioRxiv - Microbiology 2019Quote: ... or T1L/T3D M2 μ1 M258I σ3 S344P virions (2×1012 particles/ml) were incubated in the presence of 10 μg/ml endoproteinase LysC (New England Biolabs) at 37°C in a S1000 thermal cycler (Bio-Rad) ...
-
bioRxiv - Biochemistry 2019Quote: ... Washed resins were resuspended in 1 CV of buffer A and mixed with 10xHis-tagged Gi heterotrimer at the ratio of 2 mg Gi per ml resin with the presence of 25 mU/ml apyrase (New England Biolabs). The resuspended resin/Gi mixture was subjected to irradiation with 495-nm long-passed filter for 10-15 minutes to induce activation of rhodopsin and G protein binding ...
-
bioRxiv - Molecular Biology 2020Quote: ... Ligation Test 1 was processed directly to index PCR while product in Ligation Test 2 was digested by 12.5 units of RNase H (NEB, Cat.No.M0297) at 37°C for 20min before index PCR.
-
bioRxiv - Microbiology 2019Quote: High throughput sequencing libraries were prepared using RNA isolated from PAR-CLIP samples using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 2; cat# E7580, New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... We treated DNA extractions with 2 μl of RNAase A (ThermoScientific) and purified them with Monarch PCR & DNA Cleanup Kit (New England Biolabs). We assessed DNA quantity and quality with Qubit 3.0 (Thermofisher) ...
-
bioRxiv - Immunology 2019Quote: ... Restriction enzyme digests were then performed using bsII at 55° for 2 hours (for cxcr4b) and bstXi (New England Biolabs) at 37° for 2 hours (for cxcl12a) ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were washed once more with PBS and reincubated with complete medium with 2 μM BTP (SNAP-Cell Block; New England Biolabs) for 30 minutes and again washed with PBS and further treated for analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by annealing and ligation with a DNA/RNA-hybrid stem-loop adapter using T4 RNA ligase 2 (New England Biolabs), which specifically ligates the adapter to mature tRNA ...