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Citations for New England Biolabs :
1601 - 1650 of 3540 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... the plug was incubated in 160 μl of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England BIolabs) overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... From the resulting plasmid a fragment containing CRE-bGH-poly(A) was excised with restriction enzymes SalI (NEB, R3138S) and AleI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 μg of 32P-labelled R-loop-containing plasmid was either mock-treated or incubated with 0.5 U RNase H (NEB) in 75 mM KCl / 50 mM Tris-HCl pH 7.5 / 3 mM MgCl2 / 10 mM DTT in a 20 μL reaction volume for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer, 0.4 μl NEB Quick Ligase ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence in a reaction containing 40 ng/μl DNA (4 μg total) and 0.5 U/μl restriction enzyme (HindIII-HF; NEB, Cat ...
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bioRxiv - Microbiology 2020Quote: ... regions of ~600 bp containing the sgRNA sequence were amplified using NEBNext Ultra II Q5 Master Mix (NEB #M0544S) in 25 amplification cycles ...
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bioRxiv - Immunology 2020Quote: ... annealed oligos containing the sgRNA targeting sequence were phosphorylated and cloned into a dephosphorylated and BsmBI (New England Biolabs) digested SgOpti (Addgene#85681 ...
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bioRxiv - Molecular Biology 2021Quote: ... Each amplification template containing appropriate copy number of the gene was added into the master mix containing 1 μL of Bst 3.0 DNA polymerase (Cat. No. M0374L, NEB), 2.5 μL of 10 × isothermal amplification buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the first step PCR reaction was performed in a 12.5 μl mixture containing 1X OneTaq Hot Start Master Mix (NEB), 0.4 μM of each DHPV-U 1538F and DHPV-U 1887R primer and 20 ng of DNA template ...
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bioRxiv - Microbiology 2021Quote: ... containing mRNA was isolated from 1μg total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Immunology 2020Quote: ... annealed oligos containing the sgRNA targeting sequence were phosphorylated and cloned into a dephosphorylated and BsmBI (New England Biolabs) digested SgOpti (Addgene#85681 ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were covered with 30 μL of reverse transcription mixture containing dNTPs (0.5 mM each, New England BioLabs), acetylated BSA (1,5 μg) ...
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bioRxiv - Molecular Biology 2023Quote: ... ddH2O and 2μL of diluted cDNA were used in 10μL qPCR reactions containing the Luna® Universal qPCR Master Mix (NEB) and 0.3μM of gene-specific forward and reverse primer ...
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bioRxiv - Genomics 2022Quote: ... and inserting a 1969bp custom gBlock (IDT) containing EcoRI-myrGFP-StuI-Dam-MCS-Xbal via NEB HiFi assembly (NEB). The myrGFP sequence represents the first 85aa of D ...
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bioRxiv - Molecular Biology 2023Quote: ... each plug was incubated in 160 μl of 1x NEBuffer 3.1 containing 1 unit/μl of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Specific mutations in human PSEN1 were obtained by Q5 site-directed mutagenesis of the pMSCV-puro vector containing wild type human PSEN1.26 We designed primers for Q5 site-directed mutagenesis (New England Biolabs) with NEBaseChanger.neb.com and performed site-directed mutagenesis according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μl of a mix preheated to 37°C and containing 2.5 U of RNase H (New England Biolabs), 2.5× RNase H reaction buffer (1× buffer ...
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bioRxiv - Cancer Biology 2023Quote: Plasmids containing the “circularization cassettes” were generated by using NEBuilder HiFi DNA Assembly Cloning Kit (#E5520S; New England BioLabs) and KLD Enzyme Mix (#M0554S ...
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bioRxiv - Immunology 2023Quote: ... Amplicon containing U6 promoter and sgRNA4 was PCR amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs, M0530) and assembled into SalI linearized LentiGuide-GBP-Chr3 sgRNA3 plasmid using HiFi DNA Assembly Kit (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... were single-cell sorted into 5 µl 1% (v/v) Nonidet P40 Substitute, Tris-HCl (20 mM, pH 8.0) containing 5 U murine RNase inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
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bioRxiv - Bioengineering 2023Quote: ... reactions were carried out in a total of 25 μL containing 1 X Isothermal Amplification Buffer (New England Biolabs), 5 mM MgSO4 ...
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bioRxiv - Genomics 2023Quote: ... nuclei were sorted into a tube of a PCR tube strip containing 5 μL 1X CutSmart buffer (NEB, B7204S) per well ...
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bioRxiv - Bioengineering 2023Quote: ... previously described 34,51 containing the tetracycline resistance cassette and sacB3 gene via a three-fragment HiFi reaction (New England Biolabs). Escherichia coli S17-1 was transformed with the pMTL70641:ΔpilA construct and conjugated with C ...
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bioRxiv - Neuroscience 2023Quote: ... DNA ligation was performed in the same reaction mixture containing the phosphorylated samples using 5U T4 DNA ligase (NEB). A transformation assay was performed using 3 μL DNA ligation product in DH5alpha bacterial cells and the positive clones were verified by Sanger sequencing.
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bioRxiv - Neuroscience 2023Quote: ... sense and antisense oligos containing the 20 bp guide target sequence wereannealed and phosphorylated with T4 Polynucleotide Kinase (NEB), then inserted between BbsI sites in the plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... The next day samples were washed twice with dig-wash (wash buffer with 0.025% digitonin) and then resuspended in 150 µl dig-wash containing pA-MNase (New England Biolabs 700 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... were generated using the vPRC1.4 recombined plasmid (containing all three expression cassettes) as template and using Phusion polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Mutant variants of the mS29-containing construct were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). Mutagenesis primers were designed using the NEBaseChanger tool ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: A 10 µL reaction containing 200 ng of pTrc200HA plasmid DNA and 1× CutSmart Buffer (New England BioLabs, USA) with partially purified V2c or its variants normalized by the major V2c protein band was incubated at 37°C for 1 hour ...
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bioRxiv - Genomics 2024Quote: ... were carried out for a final volume of 12.5 µL containing 1x OneTaq HS Quick-Load Master Mix with Standard Buffer (NEB), 0.2 µM of each forward and reverse Intron-flanking primers (Supplementary Table 7) ...
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bioRxiv - Molecular Biology 2024Quote: ... Genes of interest were cloned into Bxb1 attB-containing plasmids using standard Gibson assembly cloning techniques (NEB cat #E2621L). To stably recombine the attB plasmids under control of the landing pad promoters ...
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bioRxiv - Neuroscience 2024Quote: ... brains were incubated in PBT (0.3 % Triton X-100 in PBS) containing CLIP-Surface 547 substrate (1µM, S9233S; New England Biolabs Inc.) for 15 min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... of PCR products carrying complementary USER junctions were mixed in a 10 μl reaction containing 0.75 units DpnI (New England Biolabs), 0.75 units USER enzyme (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... pellets dried and resuspended in 100 µl RNase-free water containing 40 U murine RNase inhibitor (New England Biolabs). Following quantification by Nanodrop ...
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bioRxiv - Molecular Biology 2024Quote: ... the previous step repeated then the pellets resuspended in ice cold NP-S containing 4U MNase (New England Biolabs) for 10 mins at 25 °C ...
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bioRxiv - Microbiology 2024Quote: ... The linearized plasmid containing sgRNA (∼10 kb) was purified and fragments flanking gadB were ligated using Gibson assembly (NEB). The primer and sgRNA sequences are listed in Table S1.
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bioRxiv - Synthetic Biology 2024Quote: ... approximately 0.5µg plasmid DNA was added into the digestion mixture containing 1.5µL rCutSmart™ Buffer (New England Biolabs, Cat# B6004S), 10 units of each restriction enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA from each phosphatase or control reaction was then used to set up two reactions 20 μL reactions containing 1 μg of treated RNA in NEBuffer 3.1 (New England Biolabs) with or without the addition of 1 μL (1U ...
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bioRxiv - Plant Biology 2024Quote: Mutations (D to V) of the MHD motif were carried out using inverse PCR with primers containing AarI (NEB) or BsmBI (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... a vector containing an N-terminal Thioredoxin-His10 tag using a Gibson Assembly® Cloning Kit (New England BioLabs). Plasmids were extracted and transformed into competent E ...
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bioRxiv - Neuroscience 2024Quote: ... the gel was immersed in 0.3 M sodium acetate solution containing 20 Unit RNase inhibitor (New England Biolabs, M0314L) overnight at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... Supernatant containing 200-500 bp DNA sizes was separated from beads using 12-tube magnetic separation rack (NEB # S1509S) and collected ...
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bioRxiv - Genomics 2024Quote: ... All the ligation products were used as templates for RT in a final 30 μl reaction volume containing 8 U of Bst 3.0 DNA Polymerase (8 U/μL, NEB), 1X RT reaction buffer ...
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bioRxiv - Genomics 2024Quote: ... a 16 hour RCA reaction was performed at 30°C using a mixture containing 500 µM dNTP (NEB #N0447S), 50 µM amino-allyl-dUTP (Thermo Scientific #R1091) ...
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bioRxiv - Developmental Biology 2024Quote: Tissue sections were initially washed with 0.1× SSC buffer (Thermo, AM9770) containing 0.05 U/ml RNase inhibitor (NEB, M0314L) to remove photographic staining ...