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Citations for New England Biolabs :
1401 - 1450 of 3540 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... the protein was mixed with Quick Calf Intestinal Alkaline Phosphatase (New England BioLabs) for 1 hour at room temperature to be dephosphorylated ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound antibodies were detected by incubation with anti-rabbit or anti-mouse DyLight 800-conjugated secondary antibodies (New England BioLabs). Slide images were acquired using an InnoScan 710-IR scanner (Innopsys ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies used were: New England Biolabs (NEB), Hitchin ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-MBP antibody (NEB E8032L; 1:10,000) in 1x TBST supplemented with 5% low-fat milk were incubated either at 4 °C overnight or 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse antibodies against MBP (NEB E8032L, 1:20,000) were used to detect 2XMBP-tagged proteins and rabbit antibodies against HA (Cell Signaling C29F4 ...
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bioRxiv - Cancer Biology 2024Quote: The rabbit anti-m6A antibody (#E1610S, NEB, U.S.A) was conjugated to Protein A agarose beads (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Sac II restriction enzymes (NEB) and cloning each promoter ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This was then loaded and incubated in a gravity column containing 25 mL of chitin bead slurry (NEB), then washed with 400 mL column buffer (20 mM TrisHCl pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... primers containing overhangs of the 3x FLAG peptide were phosphorylated using the T4 polynucleotide kinase (New England BioLabs). Phosphorylated primers were used to PCR amplify pQLinkN-pgaD to generate the plasmid pQLinkN-pgaD-FLAG ...
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bioRxiv - Systems Biology 2020Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA-bound beads were resuspended in 100µl of mix containing 82µl of 1X NEB T4 DNA ligase buffer with 10mM ATP (NEB), 10µl of 10 (2.5mM each ...
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bioRxiv - Genetics 2021Quote: ... Different SCON variant fragments containing the respective complementary ends were then inserted into eGFP with SapI (R0569S, NEB) and T4 ligase for 20 cycles of 2 minutes at 37 °C and 5 minutes at 16 °C ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 1 μM uracil-containing oligonucleotide with 0.6 U UDG (New England Biolabs) (55 ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was used as template in PCR reactions containing the Taq 2X Master Mix (New England Biolabs) and 5 μM of MP2- and scorpine-specific primers (Table S7) ...
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bioRxiv - Immunology 2022Quote: Plasmids containing templates for CRISPR-mediated KI were assembled using the NEBuilder HIFI assembly kit (New England Biolabs) starting from PCR amplified fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... EcoRV de-staining buffer (deionized water containing 1X cut-smart buffer and 200 units of EcoRV (NEB, #R3195S) in 500ul) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNase I de-staining buffer (PBS containing 1X DNase buffer and 20 units of DNase I (NEB, #M0303) in 500ul) ...
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bioRxiv - Microbiology 2021Quote: ... Transposon-containing fragments were enriched by PCR using ComP7 primers ComP5 using Phusion DNA polymerase (New England Biolabs) in a 20-cycle reaction ...
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bioRxiv - Microbiology 2021Quote: ... The PCR for each sample was performed in 50µl total volume: containing 0.5µl Q5 High-Fidelity DNA polymerase (New England Biolabs, USA), 10µl 5X Q5 buffer ...
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bioRxiv - Genomics 2021Quote: ... 1uL of the oligo mix was added to a master mix containing 1x T4 ligase buffer (NEB M0202M), 0.5 uL T4 PNK (NEB M0201L ...
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bioRxiv - Genetics 2021Quote: ... We then created a construct containing the alternate allele using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were:
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bioRxiv - Biochemistry 2021Quote: ... fully assembled complexes containing His-Rpn12 and MBP-Rpn6 were purified using a HisTrap and amylose resin (NEB), and cleaved with HRV-protease ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer ...
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bioRxiv - Genomics 2021Quote: ... in a 25 μL reaction containing 1X PNK buffer and 15 units of polynucleotide kinase (New England Biolabs) by incubating at 37°C for 45 minutes ...
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bioRxiv - Genomics 2020Quote: ... A construct containing the alternate allele was then created using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were as follows:
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bioRxiv - Immunology 2020Quote: ... before amplification with Illumina adapter-containing primers (Nextera i7 indices) and NEBNext Ultra II Q5 Master Mix (NEB) as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in Fast AP Master Mix (1x Fast AP Buffer containing 80U RNase Inhibitor (M0314, NEB), 2U TURBO DNase (AM2238 ...
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bioRxiv - Immunology 2021Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... MYH7 fragment containing mutation was amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) and 500 nM forward and reverse primers (Table 3) ...
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bioRxiv - Biophysics 2023Quote: ... p-SFG retroviral backbone containing an IRES-ΔCD19 reporter gene was linearized by NotI-HF (NEB, ref: R3189S) and XhoI (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... and beads resuspended in a 50-µl PCR mix containing 1x NEBNext High-Fidelity PCR Master Mix (NEB) and 0.5 µM of Nextera Ad1_noMX and Ad2.X primers ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA containing one copy of the SIV gene was linearized using EcoR1 (New England Biolabs, Ipswich, MA) following their restriction digest protocol (New England BioLabs ...
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bioRxiv - Microbiology 2022Quote: ... pRS1 containing the YohP-MS2-6xbs coding sequence was first linearized by EcoRI restriction digest (NEB, Frankfurt, Germany). In vitro transcription was performed using AmpliScribe T7-Flash Transcription kit (Lucigen Corp. ...
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bioRxiv - Microbiology 2023Quote: NINL (1-702) containing an amino-terminal HaloTag was expressed in BL-21[DE3] cells (New England Biolabs), which were then grown until OD 0.4-0.6 and induced with 0.1 mM IPTG for 16 hr at 16°C ...
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Oligos containing the spacer sequence and Esp3I sticky ends were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) and purified using the Oligo Clean and Concentrator kit (Zymo Research) ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... coli in vitro transcription were synthesized through a 200 µL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 µM dNTP Mix (NEB ...
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bioRxiv - Biophysics 2023Quote: A Tandem HaloTag plasmid containing the HaloTag-GTGSGSGS-HaloTag cDNA sequence was built via Gibson Assembly (NEB, (62)) of two HaloTag genes ...
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bioRxiv - Molecular Biology 2023Quote: ... Such probes were generated by linearizing pDONR221-vectors containing the DsuzIR62a-coding sequences using NheI (New England Biolabs) following protocols described above ...
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bioRxiv - Microbiology 2024Quote: ... TAP-PCR was performed in a total volume of 25 μL containing 0.25 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... A mixture containing 1 μl of a 4-fold dilution of Low Range ssRNA Ladder (New England Biolabs) and 0.75 pmol each of RPIX_SC1_Bridge ...
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bioRxiv - Physiology 2023Quote: ... The PCR was performed in a total volume of 50 µl containing 1× HF buffer (New England BioLabs), 1 µl dNTP Mix (New England BioLabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was then replaced with a fresh imaging medium containing 1 µM SNAP Cell Block (NEB, S9106S) to prevent the binding of residual SNAP dye to newly synthesized SNAP-tagged proteins ...
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bioRxiv - Microbiology 2024Quote: ... 3 μL of a ligation mixture containing 0.01 U of Bst 2.0 DNA polymerase (NEB, Ipswich, MA, USA), 0.5 U of SplintR ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were carried out in 20 μl reaction solutions containing 1X Luna® Universal qPCR Master Mix (NEB), 20 ng cDNA and 0.25 μM each gene-specific primer ...
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bioRxiv - Genomics 2024Quote: ... A 50 µL PCR reaction was prepared containing 1X NEBNext® High-Fidelity PCR Master Mix (NEB #M0541L), 1 µM Ex situ custom forward primer ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic region containing the CRISPR-edit was PCR-amplified using OneTaq polymerases in Standard Buffer (New England Biolabs). After PCR ...