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Citations for New England Biolabs :
1551 - 1600 of 2481 citations for Dengue Virus Serotype 2 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Biochemistry 2019Quote: ... All sens RNA strands were labeled at their 5’ end with [γ-32P] ATP using protein nucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... 115 amino acids) fused to the C-terminus of maltose binding protein (MBP) in pMALX vector (New England Biolabs), MBP-5-HT3A-ICD-pMALX ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of genomic DNA was incubated with 3 µg of in vitro transcribed sgRNAs and 3 µg of purified Cas9 protein in 20µl of 1x NEB3 buffer (New England Biolabs) at 37°C over night ...
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bioRxiv - Developmental Biology 2019Quote: ... single copy scGESTALT.2 F1 male transgenic adults were crossed to wildtype female adults and one-cell embryos were injected with 1.5 nl of Cas9 protein (NEB) and sgRNAs 1-8 in salt solution (8 µM Cas9 ...
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bioRxiv - Microbiology 2021Quote: ... the nuclei were pelleted and supernatants were transferred to 2.0 ml screw-top tubes with O-rings containing magnetic beads (SureBeads Protein G Magnetic Beads, NEB) coupled to antibodies (anti-pan Ago antibody ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 750 µl ligation mixture containing 200 units T4 DNA ligase (NEB/ IMB core facility protein production), 1X T4-Ligation buffer (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: The total volume of CRISPR Cas12a-based cleavage assay was 20 μL including LbCas12a protein (New England BioLabs, Inc.), crRNA ...
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bioRxiv - Cancer Biology 2019Quote: ... 25ug of each protein lysate were diluted into 3xSDS gel loading buffer (New England Biolabs Cat# B7703S, Lpswich, MA) and heated to 95°C for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The purified target PCR amplicon was incubated with purified recombinant wt- or en-Cas12a protein and crRNA (RNA-guides or chimeric DNA-RNA guides) in 10X buffer (NEBuffer3.1, NEB) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of each sgRNA and 60 pmol Cas9-NLS protein (EnGen® Spy Cas9 NLS, NEB, M0646 M) with the Lonza 4D X-unit ...
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bioRxiv - Genetics 2021Quote: ... Five µl of the hybridization product was combined with 1 µl of 20 µM Cas9 protein (New England Biolabs) at room temperaturefor 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Expression of all SurA and SurA-OmpA proteins was carried out using chemically competent home-made stocks of E.coli strain BL21(DE3) originally sourced commercially (NEB). Both proteins were over-produced separately in 1 L of cultures as described previously [42] ...
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bioRxiv - Biochemistry 2022Quote: ... Expression of all soluble proteins used chemically competent home-made stocks of E.coli strain BL21(DE3) originally sourced commercially (NEB).
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bioRxiv - Cell Biology 2022Quote: ... Protein dephosphorylation was achieved by incubation of 20 μg sample with 400 units of λ phosphatase (New England Biolabs) in 10X NEBuffer for protein MetalloPhosphatases (PMP ...
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bioRxiv - Molecular Biology 2022Quote: ... a mixture of 0.5 μg of IMAC purified protein and 0.5 μg BSA (New England Biolabs, Ipswich, MA, USA) in 100 μl of coating buffer per well was added to 384-well microtiter plates (1 μg without BSA for direct detection) ...
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bioRxiv - Biophysics 2022Quote: ... and hCFTR-containing fractions based on SDS-PAGE analysis were pooled after thawing and immediately phosphorylated with 1,000 U of protein kinase A (New England BioLabs) per ml for ∼1 hr on ice prior to addition of VX770 and VX445 to some samples and final purification via Superose 6 Increase 3.2/300 gl microbore gel-filtration chromatography in glycerol-free TMN buffer with 150 µM ATP ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x 106 PDCL-1108 cells were electroporated with 10 μg spCas9-NLS protein (New England Biolabs, Ipswich, MA), 4 μg guide RNA and 200 pmol ssODN using the Amaxa Nucleofector II (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: The sf-GFP reporter protein was expressed essentially as described 38 in the PURExpress ΔRF123 system (New England Biolabs). The DNA template was PCR-amplified from the pY71sfGFP plasmid 58 by PCR using primers sfGFP-fwd and sfGFP-rev (all primers are listed in Supplementary Table S2) ...
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bioRxiv - Biochemistry 2023Quote: Trx-linker concatemers (1 mg/mL) were incubated with 50,000 units of the catalytic subunit of cAMP-dependent protein kinase (PKA) (NEB)—which recognizes the RRAS motif within the central linker of the Trx-linker nonamer—in protein kinase buffer (50 mM Tris HCl ...
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bioRxiv - Cell Biology 2023Quote: ... total proteins isolated from cell lysates were digested with Endoglycosidase H (endo H) enzyme (New England Biolabs, catalog #: P0703L) with G5 reaction buffer at 37°C according to manufacturer’s instruction and the published procedure 15 ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... histidine tags were cleaved with LysC (200 ng/mg of protein; two hours at room temperature; New England Biolabs), and for all others ...
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bioRxiv - Cell Biology 2023Quote: ... Total lysates (200 µg each) were incubated with or without 400 U lambda protein phosphatase (New England Biolabs, #P0753S) and 1 mM MnCl2 at 30 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Purified and concentrated mTAAR7f was mixed with about 10x molar excess of both the heterotrimeric G protein and Nb35 and 0.5 U apyrase (NEB) and incubated overnight ...
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bioRxiv - Immunology 2023Quote: ... Glycan removal was performed on 20 µg or 25 µg total protein using PNGase F (#P0704; New England Biolabs), Endo H (#P0702 ...
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bioRxiv - Developmental Biology 2024Quote: ... A cocktail of guide RNAs for each target (1 μL each) was pre-incubated with Cas9 protein (NEB #M0646M) and 0.68uL KCl (as described in (100)) ...
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bioRxiv - Biophysics 2023Quote: ... We then sequentially labeled Nup96 proteins with SNAP ligand conjugated Alexa Fluor 647 (BG-AF647, S9136S, New England Biolabs) and mitochondria with primary anti-Tomm20 antibodies (rabbit polyclonal ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing CrtSPARTA protein complex was pooled and further loaded onto an amylose resin column (New England Biolabs). The column was washed with 20 column volumes (CV ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng of each integrase amplicon was used per reaction using PURExpress® In Vitro Protein Synthesis Kit (NEB) in a total volume of 9 µl ...
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bioRxiv - Cell Biology 2023Quote: For protein synthesis the verified plasmid was transformed into BL21 competent cells (C2530, New England BioLabs, Ipswich, MA, USA). Cells were grown shaking at 37°C in 2XYT with appropriate selection to an OD600 of 0.6 and then transferred to 18°C ...
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bioRxiv - Microbiology 2024Quote: ... protein aliquots were subjected to PNGase F or a broad range mannosidase (α1,2/3/6) (New England Biolabs, France) digestion according to the manufacturer’s specifications ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.5 µL of 10X GlycoBuffer 3 (Cat.#B1720S; New England Biolabs) and 2.5 µL of Endo Hf in a total reaction volume of 25 µL ...
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bioRxiv - Developmental Biology 2024Quote: ... a mix containing 18µM sgRNA and 12µM Cas9 protein (EnGen™ Spy Cas9-NLS S. pyogenes, NEB Cat# M0646T) was incubated for 10 min at room temperature ...
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bioRxiv - Biophysics 2024Quote: M2R constructs were dephosphorylated through a 30-minute room temperature treatment with 200 units of lambda protein phosphatase (NEB), 10 units of calf intestinal phosphatase (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... MBP tags that co-eluted with NTF2L proteins were purified by incubating with amylose resin (New England BioLabs, #E8021L) overnight at 4°C and collecting the flow through the following day ...
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bioRxiv - Microbiology 2024Quote: ... the protein eluted from MonoQ 5/50 GL was incubated with 10 μM SNAP-Surface Alexa Fluor 647 (NEB) for 1 hour at 4°C prior to gel filtration.
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bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.2 μM primers (primer sequences are presented in Table S1) and 2 × PCR Master mix (M0482S, NEB), with the following cycling parameters ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Extracted RNA was treated with 2% RNase-free DNase I (New England BioLabs Japan Inc., Tokyo, Japan) at 37°C for 40 minutes and purified by phenol/chloroform extraction and ethanol precipitation.
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 2 nM HaloTag-JF549 and 5 nM SNAP-Cell® 647-SiR (NEB) before imaging.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... 7 μl PEG 8000 (17.5% final) and 1 μl of T4 RNA ligase 2 KQ (NEB, M0373L) in 1X T4 RNA ligase buffer ...
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bioRxiv - Genomics 2019Quote: ... by adding 20 µL of ligation mix [2 µL of [10X Taq DNA ligase buffer (NEB B0208S), 0.25 µL Taq DNA ligase (NEB M0208S) ...
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bioRxiv - Genomics 2019Quote: ... DNA fragment size was determined by running 2 µL of DNA along with low molecular ladder (NEB) on a 2% agarose gel.
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bioRxiv - Microbiology 2019Quote: ... PCR was performed on a plasmid template using the Q5 High-fidelity 2× Master Mix (NEB, M0492L), with primers at final concentration of 500nM ...
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bioRxiv - Microbiology 2021Quote: ... total RNAs were extracted from Caco-2 cells and followed by mRNA purification (Oligo dT beads, NEB). The mRNAs were fragmented and converted into double-stranded DNA by reverse transcription and then second-strand synthesis (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... 8 μL of the de-phosphorylated DNA was incubated with 2 μL T4 Polynucleotide Kinase (NEB M0203S), 3 μL γ32P-dATP (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified PCR products were denatured and reannealed in NEBuffer 2 (New England BioLabs, Ipswich, MA, USA), using a thermocycler ...
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bioRxiv - Neuroscience 2021Quote: ... 50mM Sodium Butyrate) and incubated in a 37C water bath with 2 microliters Micrococcal Nuclease enzyme (NEB) for eight minutes ...