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Citations for New England Biolabs :
1551 - 1600 of 10000+ citations for Cow Casein Kinase II Subunit Alpha 2 CSNK2A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The antisense strand with a 3′ phosphate was radiolabeled by T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB) and [γ-32P]ATP ...
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bioRxiv - Microbiology 2020Quote: The 3′ ends of the ribosome footprint RNA fragments were then treated with T4 polynucleotide kinase (NEB) to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr ...
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bioRxiv - Biochemistry 2020Quote: ... T4 polynucleotide kinase (T4 PNK) used in EMSAs and all other enzymes mentioned were purchased from NEB and used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The minimal promoter oligos were phosphorylated and annealed using T4 Ligation Buffer and T4 Polynucleotide Kinase (NEB) at 37 °C for 30 minutes followed by 95 °C for five minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... 400 ng of eluted DNA fragments were end-labeled with T4 Polynucleotide Kinase (T4 PNK) (NEB M0201L) and 10 µCi of [α-32P]-ATP (Perkins Elmer NEG002A ...
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bioRxiv - Biochemistry 2021Quote: Radiolabeling of oligonucleotides was carried out for 1 h at 37 °C using T4 polynucleotide kinase (NEB), 0.25 μM oligonucleotide ...
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bioRxiv - Cell Biology 2022Quote: ... Hybridized oligonucleotides were phosphorylated with 5 U T4 polynucleotide kinase in T4 Ligation buffer (New England Biolabs) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Table 4 was end labeled using gamma-ATP and T4 Polynucleotide Kinase radioactive labeling protocol from NEB. Labelled oligos were purified using GE Healthcare illustra ProbeQuant G-50 Micro Columns and the membrane was probed overnight ...
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bioRxiv - Synthetic Biology 2021Quote: ... oPool oligos were phosphorylated at their 5’ ends using T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA). DNA adapters were then annealed to the phosphorylated oPool oligos by incubating extension ...
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bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
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bioRxiv - Microbiology 2022Quote: ... polynucleotide kinase (PNK) and Antarctic phosphatase used during cloning were all purchased from NEB (Ipswich, MA, USA). Purification of PCR products was carried out using the Qiagen PCR purification kit according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... Fresh cultured cells were used for total genomic DNA extraction and the T4 polynucleotide kinase (Biolabs #M0201S) concentration was adjusted to 100U and incubated for 30 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Two annealed oligonucleotides containing the homology region were phosphorylated by T4 polynucleotide kinase (New England Biolabs, M201) and cloned into the vectors pX335 and pKN7 (see Appendix Table S1 ...
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bioRxiv - Biochemistry 2023Quote: ... Dephosphorylated guide RNAs were then radiolabeled using T4 Polynucleotide Kinase (0.33 U/µL, New England Biolabs, M0201) and 0.42 µM [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’ ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) and ligated to a pre-adenylated 3’ adapter (L3-App) ...
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bioRxiv - Biochemistry 2023Quote: ... The RNA fragments purified from 48S PIC RPFs were dephosphorylated using T4 Polynucleotide Kinase (PNK) (NEB; M0201L) and ligated to pre-adenylated linkers (NI-810 to NI-815 ...
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bioRxiv - Molecular Biology 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’ ends using T4 Polynucleotide Kinase (New England Biolabs, M0201S) and ligated to a pre-adenylated 3’ adapter (L3-App) ...
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and a 50 pm RNA probe was incubated with 20U T4 Polynucleotide kinase (PNK) (NEB, catalogue # M0201S), 1X T4 PNK buffer and 30 µCi P32ATP at 37°C for 30minutes (reaction volume 50µl ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified and desalted ligation product was then electroporated into high efficiency 5-alpha E.coli cells (NEB). The cells were grown overnight on LB-Carbenicillin plates and then ...
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bioRxiv - Biochemistry 2022Quote: ... Finale expression constructs were subcloned in NEB 5- alpha competent E.coli cells (New England Biolabs, Ipswich, US) followed by Sanger sequencing (Microsynth ...
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bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Microbiology 2019Quote: ... (2) DSBs blunting with NEB’s Quick Blunting Kit (NEB, cat. number: E1201); (3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µM IllLigBio adapter pre-adenylated using a 5’ adenylation kit (NEB), 20-200 nM pppUGAAUG hexamer standard ...
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bioRxiv - Microbiology 2021Quote: ... was utilized using SARS-CoV-2 specific primers[43] or SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit (New England Biolabs), which became available in assays after September 15 ...
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bioRxiv - Microbiology 2022Quote: ... human NINL isoform 2 (NCBI accession NM_001318226.2) and the NINL isoform 2 mutant (Q231R) were mutagenized using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). The plasmids encoding 3C proteases (coxsackievirus B3 (CVB3 ...
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bioRxiv - Cancer Biology 2023Quote: ... and ligated to hairpin adaptor 2 (ENDseq-adaptor-2, 5′-Phos-GATCGGAAGAGCACACGTCUUUUUUUUAGACGTGTGCTCTTCCGATC*T-3′ [*phosphorothioate bond]) (Quick ligation kit, NEB). To prepare libraries for sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Adaptors were removed from the 3’ end of reads using cutadapt (Martin ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA-Seq libraries (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, New England BioLabs, Catalog No. E7760) were produced from 16 samples according to the manufacturer’s protocol using 200 ng total RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... and PCR addition of indices (7-11 cycles) was done using NEBNext Ultra II DNA library prep kit (NEB). Biotinylated capture probes 70 nt in length were designed against every DpnII restriction fragment in a 2.5 Mb window centered on Runx1 (chr16:91,566,000-94,101,999) ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645) according to protocol ...
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bioRxiv - Plant Biology 2021Quote: ... was used to generate libraries with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Cat# 7645). The resulting libraries were sequenced (single end 50 bp ...
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bioRxiv - Molecular Biology 2021Quote: ... The library preparation was done using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S). The libraries were multiplexed (six samples per lane ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and sequencing libraries were prepared using the NEBNext® Ultra™ II DNA Kit (New England Biolabs, Massachusetts, USA) with a modified protocol to account for fragmented DNA ...
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bioRxiv - Microbiology 2021Quote: ... and adapter ligation reactions were performed on the fragmented DNA using the NEBNext Ultra II kit (New England Biolabs). Illumina paired-end sequencing was performed on a NextSeq500 device at the Georgia Genomics Facility (University of Georgia).
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bioRxiv - Evolutionary Biology 2021Quote: ... Libraries were constructed with the NEBNext® Ultra™II DNA Library Preparation kit (New England Biolabs Inc, USA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs #E7805) and Multiplex Oligos for Illumina (NEB #E7500) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for mRNA purification followed by a NEBNext® Ultra(tm) II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: Libraries for next-generation sequencing were prepared using the NEBNext Ultra II DNA library prep kit (New England Biolabs) following the manufacturer’s instructions and sequenced on an Illumina NextSeq 500 platform (75 bp reads ...
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bioRxiv - Molecular Biology 2021Quote: ... Second end processing and library amplification were performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... fragmented and processed into an Illumina sequencing library using the NEB Ultra II FS kit (NEB cat. no. E7805S). Libraries were paired-end sequenced on an Illumina HiSeq 4000 instrument.
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bioRxiv - Cell Biology 2022Quote: IP samples were prepared for high-throughput sequencing by using the Ultra II DNA Library Prep kit (NEB E7645L). Samples were multiplexed and paired-end sequencing was performed using a MiniSeq (Illumina ...
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bioRxiv - Genomics 2022Quote: ... sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, MA). Library preparation and sequencing were performed at the University of Missouri Genomics Technology Core ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Intact total RNA was reverse transcribed into cDNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) according to manufacturer’s instructions with random primers ...
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bioRxiv - Genomics 2019Quote: ... Strand-specific sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries from two biological replicates were sequenced on the Illumina NextSeq 500 platform resulting in 22-25×106 single-end reads ...
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bioRxiv - Genomics 2020Quote: Micro-C sequencing library was generated by using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) with some modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... we followed the manufacturer’s protocol for the NEBNext® Ultra™ II Directional RNA Library Prep kit (NEB, E7765S) and poly(A ...
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bioRxiv - Genomics 2019Quote: ... 16 libraries for each histone mark) using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, cat. # E7645). NEBNext Multiplex Oligos for Illumina (NEB ...