Labshake search
Citations for New England Biolabs :
101 - 150 of 628 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... HEK293 DNA was extracted and amplified by Q5 High-Fidelity PCR (NEB, M0494S) using primers encompassing the sgRNA recognition site ...
-
bioRxiv - Microbiology 2020Quote: ... rabbit anti-SNAP-tag (P9310, New England BioLabs), rabbit anti-beta-tubulin (NB600-936 ...
-
bioRxiv - Cell Biology 2021Quote: ... The SNAP tag (New England Biolabs, Ipswich, MA) was amplified by PCR with compatible cut sites (HindIII and BamHI ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The SNAP tag sequence was obtained from NEB vector pSNAPtag(T7_2 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... SARS-CoV-2 RNA was replaced with nuclease free water (NEB) for the assay without the target RNA ...
-
bioRxiv - Cell Biology 2021Quote: ... 20µg of protein lysates and media samples were incubated with glycoprotein denaturation buffer at 95°C for 10 min and treated with 0.3µl Endo H (NEB; Catalog no #P0702) enzyme in glyco-buffer 3 for one hour at 37°C ...
-
bioRxiv - Bioengineering 2019Quote: ... eukaryotic recombinant Stlac2 was denatured with Glycoprotein denaturing buffer at 100 °C for 10 min to be deglycosylated using PNGase F (NEB, USA) according to instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Lysates were first denatured with Glycoprotein Denaturing Buffer at 65°C for 15 min and then treated with Endoglycosidase H (Endo H) (New England Biolabs, #P0702S) or Peptide-N-Glycosidase F (PNGase F ...
-
bioRxiv - Cancer Biology 2020Quote: ... The GST tag in this construct was replaced by a 6xHis tag using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). N-PTEN was recombinantly expressed in E ...
-
bioRxiv - Plant Biology 2021Quote: ... S1 and cloned into pAGM1311 vector by Gibson assembly (New England Biolabs, Ipswich, USA). The CRISPR plasmid and circular donor plasmid were co-transformed into SG200 protoplast and mutants were singled out on PD plate with 2 μg/ml carboxin for CRISPR plasmid first then transfer to PD plate without antibiotic to get rid of CRISPR plasmid ...
-
bioRxiv - Genomics 2021Quote: ... Tables S1 and S7) using NEBNext Ultra II Q5 Master Mix (New England Biolabs). Each reaction (100 µL total volume ...
-
bioRxiv - Microbiology 2022Quote: ... dsRNA was treated by enzymatic digestion with DNaseI and S1 nuclease (New England Biolabs) to remove DNA and ssRNA from the samples ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
-
bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
-
bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
-
bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
-
bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
-
bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
-
bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
-
bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
-
bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
-
bioRxiv - Molecular Biology 2020Quote: ... with mCherry-tag by Gibson Assembly (New England Biolabs).
-
bioRxiv - Biophysics 2022Quote: ... and 0.12 µM SiR-SNAP-Tag ligand (NEB, S9102S) for 30 min at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal anti-SNAP-tag (New England BioLabs, P9310S), mouse monoclonal anti-HSP90 (BD Transduction Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-SNAP-tag antibody (New England Biolabs, Ref. P9310S), anti-clathrin heavy chain antibody (BD Bioscience ...
-
bioRxiv - Bioengineering 2020Quote: ... the SARS-CoV-2 RNA was obtained by in vitro transcription (NEB, HiScribe™ T7 High Yield RNA Synthesis Kit ...
-
bioRxiv - Biochemistry 2023Quote: ... of the SARS-CoV-2 Positive Control (N gene) plasmid (NEB N2117). Sanger sequencing confirmed successful mutagenesis ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 DNA fragment plasmids were digested with I-SceI (NEB) to release the overlapping fragments from the backbones ...
-
bioRxiv - Neuroscience 2020Quote: ... The DNA amplified fragments (Table S1) were digested with XhoI and NheI restriction enzymes (NEB). Expression of the resulting genes led to fusion proteins containing a His6 tag and a TEV Niα protease cleavage site ...
-
bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
-
bioRxiv - Plant Biology 2021Quote: ... restriction enzyme analysis (Supplemental Table S1) was performed following the manufacturer’s recommendations (NEB, Bethesda, USA). The final PCR products were analyzed via 1.5% (m/v ...
-
bioRxiv - Microbiology 2023Quote: ... was amplified from JE2 using the primer pair 3810/9647 and the snap-tag coding sequence amplified from pSNAP-tag (T7)-2 (New England Biolabs, NEB; Supplementary Information) using the primer pair 9631/9632 ...
-
bioRxiv - Genomics 2022Quote: ... we spike in 2.5 µL of 3M U/mL T7 DNA ligase (NEB M0318L) and continue incubating for 1 hour at 25C ...
-
bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
-
bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
-
bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
-
bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
-
bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
-
bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
-
bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
-
bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
-
bioRxiv - Cell Biology 2023Quote: ... The DNA fragment encoding SNAP-tag was amplified from the pSNAP-tag (m) vector (a gift from New England Biolabs & Ana Egana, Addgene #101135), and then inserted into the pDisplay vector (Invitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... Fusions to the SNAP-tag (New England Biolabs, Beverly, MA) were expressed from plasmid pAGT-Xpress ...
-
Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... SNAP-tag® ligand Blue 430 (New England Biolabs, S9109S) was applied for 30 min at a final concentration of 2 µM ...
-
bioRxiv - Biophysics 2021Quote: ... The His6-MBP tag was recaptured using amylose resin (NEB). The flow-through was loaded onto a Q-HP anion exchange column (Cytiva ...
-
bioRxiv - Systems Biology 2022Quote: ... The SNAP tag insert was derived from pSNAPf (NEB, N9183S) and the FRB-Fis1 insert was derived from pC4-RhE-FRB-Fis1 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... for Strep-tag II pull-downs and Amylose resin (NEB) for MBP pull-downs) ...
-
Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... the MPB tag was cleaved overnight using Factor Xa (NEB) in dialysis buffer and loaded again on an MBP-Trap HP column ...