Labshake search
Citations for New England Biolabs :
101 - 150 of 8756 citations for Rat Fibroblast Growth Factor 21 FGF21 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... Ribosome concentrations were calculated with a NanoDrop spectrophotometer assuming 1A260 = 60 μg ribosome mL−1 (conversion factor provided by New England Biolabs). This conversion factor was used to estimate the molecular mass of bacterial ribosomes ...
-
bioRxiv - Immunology 2023Quote: ... The resulting proCLIPB4-6His-pFastBac1 plasmid was used as a template to produce the mutant proCLIPB4Xa-6His-pFastBac1.To utilize commercial Factor Xa (New England Biolabs), the predicted activation site LTDR107 of proCLIPB4 was replaced with IEGR107 ...
-
bioRxiv - Genomics 2024Quote: Each of the fragmented protein factor-enriched chromatin sample was mixed with 50 µg/ml of BSA (cat# B9000S, NEB) to prevent chromatin aggregation ...
-
bioRxiv - Bioengineering 2024Quote: ... This fragment was cloned into a lentiviral expression plasmid we have previously described (31) encoding the Notch1 core linked to the tTA transcription factor using NEBuilder HiFi DNA Assembly (New England Biolabs). The resultant plasmid encoded constitutive expression of CII-synNotch receptor from an EF1α promoter and production of the puromycin N-acetyl-transferase transgene via the constitutive PGK promoter for antibiotic selection ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and a maltose binding protein (MBP) (65) and Factor Xa sequence derived from pMAL-c5X vector (New England Biolabs, Ipswich, MA) with a V313A mutation to be consistent with the native E ...
-
bioRxiv - Cell Biology 2022Quote: ... which was incorporated into the TMD by amber stop codon suppression in the PURExpress system lacking all release factors (ΔRF123; New England Biolabs, USA). The release factors RF2 and RF3 ...
-
bioRxiv - Plant Biology 2021Quote: ... behind the α-factor signal peptide sequence using SmaI/EcoRI restriction enzymes and T4 DNA ligase (New England Biolabs, Beverly, MA, USA). Here ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were incubated at 37ºC in complete growth medium containing either 50 nM SNAP-tetramethylrhodamine (TMR) star (New England BioLabs: S9105S) for 60 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were labelled by replacing complete growth medium with 500 μl serum free DMEM/F12 containing 200 nM SNAP Surface Alexa Fluor-488 (New England Biolabs) and were incubated for 30 min at 37°C with 5% CO2 ...
-
bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
-
bioRxiv - Systems Biology 2023Quote: ... the cultures were split and diluted to an OD750nm of 0.8 and were placed on the Cell Growth Quantifier (CGQ, Aquila Biolabs, Scientific Bioprocessing (sbi) now ...
-
bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
-
bioRxiv - Genomics 2019Quote: ... and then cloned into a customized minigene plasmid (a derivative of the pSpliceExpress vector)29 containing an RSV-promoter and two control exons (rat insulin exons 2 and 3) using the NEBuilder® HiFi DNA assembly (NEB, E2621). Amplified fragments were inserted between the two control exons ...
-
bioRxiv - Genomics 2022Quote: Ribosomal RNA depletion kit (rRNA Depletion Kit, E6310L, NEB)
-
bioRxiv - Microbiology 2023Quote: ... Monarch kit (NEB) extracted and 0.1 pmol of each DNA fragment was CPER amplified in a 50 µL reaction containing 200 µM of dNTPs ...
-
bioRxiv - Neuroscience 2021Quote: ... T7 HiScribe kit (NEB) was used to transcribe the sgRNA DNA template with the appended T7 promoter ...
-
bioRxiv - Genomics 2021Quote: ... using the Golden Gate assembly kit (NEB® Golden Gate Assembly Kit #E1601). Each guide sequence was cloned with its own U6 promoter and was followed by a sgRNA scaffold ...
-
bioRxiv - Microbiology 2023Quote: ... A RNA cleanup kit (Monarch RNA Cleanup Kit T2050, New England Biolabs, France) was used to further clean the RNA samples ...
-
bioRxiv - Synthetic Biology 2022Quote: ... plasmid (Monarch® PCR mini-prep kit) and gel extraction kits (Monarch® DNA gel extraction kit) were also obtained from NEB and used according to the manufacturer’s protocols ...
-
bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
-
bioRxiv - Developmental Biology 2021Quote: ... using HiScribe Sp6 kit (NEB) instead of T7.
-
bioRxiv - Neuroscience 2021Quote: ... rRNA depletion kit (NEB #E6310), and Ampure XP beads (Beckman #A63881) ...
-
bioRxiv - Microbiology 2020Quote: ... the PNGase kit from (NEB) was used per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... NEBNext rRNA Depletion kit (NEB) was used to remove bacterial and eukaryotic rRNA from total RNA isolated from pooled samples ...
-
bioRxiv - Genetics 2022Quote: ... HiFi Assembly Cloning Kit (NEB) to insert the LYP1 homology sequence into the BamHI/EcoRV digest of Addgene plasmid #35121 to create the final backbone plasmid BFA0190 (Supplementary Figure 10) ...
-
bioRxiv - Genomics 2022Quote: EnGen sgRNA synthesis kit (NEB)
-
bioRxiv - Genomics 2022Quote: Monarch RNA Cleanup kit (NEB)
-
bioRxiv - Microbiology 2022Quote: ... LunaScriptTMSuperMix Kit (New England BioLabs), SYBR qPCR Master Mix (ChamQTMUniversal ...
-
bioRxiv - Microbiology 2022Quote: ... or NEBase Changer–Kit (NEB) and primers listed in supplementary Table 1 ...
-
bioRxiv - Genetics 2022Quote: ... Gibson assembly cloning kit (NEB) was applied to ligate vector and GFP∷PAB-1∷3xFLAG fragment to generate plasmid pQZ2 ...
-
bioRxiv - Microbiology 2021Quote: ... The A-Tailing Kit (NEB) was then used to add additional deoxyadenosine (A ...
-
bioRxiv - Neuroscience 2022Quote: ... Quick Ligation Kit (M2200L, NEB) was used for the ligation of these appropriate clone ...
-
bioRxiv - Biochemistry 2022Quote: ... using Q5 mutagenesis kit (NEB). N-(-42D ...
-
bioRxiv - Cell Biology 2022Quote: ... using Gibson Assembly kit (NEB). To test the interaction of KRP4 and KRP5 ...
-
bioRxiv - Microbiology 2022Quote: ... LunaScript RT SuperMix Kit (NEB) was used to generate cDNA using 1μg RNA ...
-
bioRxiv - Plant Biology 2023Quote: ... blunting (Quick Blunting kit, NEB), and re-ligating using T4 DNA Ligase (NEB).
-
bioRxiv - Genomics 2023Quote: ... or RNA cleanup kit (NEB) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Q5 DNA polymerase kit (NEB) was used to make amplicons ...
-
bioRxiv - Biophysics 2023Quote: ... Gibson Assembly Kit (#E5510S, NEB) was used following the manufacturer’s instruction ...
-
bioRxiv - Synthetic Biology 2023Quote: Monarch Plasmid MiniPrep kit (NEB) for isolating template plasmid and assembled plasmid from E ...
-
bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
-
bioRxiv - Biophysics 2023Quote: ... Plasmid DNA molecules were purified using a commercially available purification kit (MonarchQR Plasmid Miniprep Kit, NEB). The purified plasmids were run on a 0.8% agarose gel supplemented with 2.5 µg/mL of chloroquine in 1xTBE buffer containing 2.5 µg/mL of chloroquine at 25 V for 15 hours ...
-
bioRxiv - Plant Biology 2020Quote: ... using the Gibson assembly kit (NEB). The pET28b vector was linearized by digesting with BamHI and XhoI to create overhangs compatible for Gibson assembly ...
-
bioRxiv - Biophysics 2020Quote: Using PURExpress kits (New England Biolabs), 37.5 μL IVT reactions were assembled on ice with 1.5 μl RNase inhibitor ...
-
bioRxiv - Microbiology 2020Quote: ... using the NEBuilder HiFi kit (NEB).
-
bioRxiv - Microbiology 2020Quote: ... using the NEBuilder HiFi kit (NEB). The mdtU-lacZ translational fusions were constructed by PCR-amplifying the mdtU gene from either a WT (E ...
-
bioRxiv - Molecular Biology 2022Quote: ... NEBNext rRNA Depletion kit( NEB #E7405) was used to deplete ribosomal RNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
-
bioRxiv - Cell Biology 2019Quote: ... using Gibson assembly cloning kit (NEB). COX8A-SNAP vector was commercially obtained from NEB ...