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Citations for New England Biolabs :
101 - 150 of 1411 citations for HLA A*0201 GP100 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... RNAsin was replaced with 10 mM ribonucleoside vanadyl complex (New England Biolabs S1402S) was added to the ground tissue ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs; Ipswich, MA) for 10–20 min in a 37°C water bath ...
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bioRxiv - Molecular Biology 2021Quote: A purified CK2 complex was purchased (#P6010S, New England BioLabs, Beverly, MA, USA). GST-mNRK565-868 ...
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bioRxiv - Microbiology 2020Quote: ... The trimer complex was then partially deglycosylated using endoH digestion (New England Biolabs) (23 ...
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bioRxiv - Molecular Biology 2022Quote: ... The Cas9/gRNA complex was generated as described by the manufacturer’s protocol (NEB). For pks1 deletion ...
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bioRxiv - Molecular Biology 2022Quote: ... all the buffers above were supplemented with 10 mM ribonucleoside-vanadyl complex (NEB) to inhibit ribonucleases ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs; Ipswich, MA) for 10–20 min in a 37°C water bath ...
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bioRxiv - Biochemistry 2022Quote: ... The enzyme complex was then further purified by incubation with chitin beads (NEB), to remove traces of TEV and fully active kinase or phosphatase ...
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bioRxiv - Neuroscience 2024Quote: ... 2U/ul RNAse inhibitor and supplemented with ribonucleoside vanadyl complex (Concentration, NEB S1402S), then incubated for 30 minutes with secondary antibodies at RT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
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bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
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bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
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bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
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bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
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bioRxiv - Developmental Biology 2021Quote: ... for 10 min with 2 mM Ribonucleoside-Vanadyl Complex RVC (New England Biolabs, S1402S) at room temperature and then permeabilized for 5 min on ice in 0.5% Triton-X with 2mM RVC ...
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bioRxiv - Molecular Biology 2021Quote: The DNA-complex-coated beads were incubated with the end-repair enzyme mixture (NEB) at 20 °C for 30 min to repair the DNA ends and then with Klenow exo- (NEB ...
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bioRxiv - Pathology 2023Quote: ... was ligated to the RNA-cDNA hybrid complex using T4 DNA Ligase (NEB M0202L) and subsequently purified with the Agencourt RNAClean XP beads (Beckman-Coulter ...
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bioRxiv - Genomics 2023Quote: ... as described before. Cas9-gRNA complex (6.6 uM S. pyogenes Cas9 Nuclease (NEB, M0386T), 20 uM gRNA ...
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bioRxiv - Microbiology 2019Quote: ... or Bam HI and Xho I (New England BioLabs, MA). The column isolated DNA fragments were ligated into our modified pFastBac1 plasmid having 6xHis tag at C-terminus ...
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bioRxiv - Molecular Biology 2020Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB). The resulting next-generation sequencing libraries were sequenced on a HiSeq2500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Followed by subcloning the PCR amplicon in Bam-HI (NEB) restriction digested pCW57-tGFP-2A-MCS plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... using NEBuilder Hi-Fi Assembly (New England Biolabs, Cat. #E2621). The pMB1052 construct was subsequently cut using BspDI and EcoRV (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... and amplified via PCR with NEBNext Hi-fidelity enzyme (NEB).
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Cancer Biology 2023Quote: ... cut with hi-fidelity EcoRI and BamHI (New England Biolabs), and gel purified ...
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bioRxiv - Molecular Biology 2023Quote: ... The Hi-Scribe T7 High Yield RNA synthesis kit (NEB) was used to generate RNA transcripts at 37°C for 16 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: Both HLS-C and HLS-E protein at 1 mg/mL were incubated with human Furin (EC 3.4.21.75, P09958, obtained from NEB P8077) in digestion buffer (10 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... 200 ng of nucleic acids from each complex were mixed in 1xDNase I buffer (NEB) with either RNase-free DNase I (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... “RRtoGG,” mutant (clasped construct used for integrin:Fab complex) was generated via site-directed mutagenesis (NEB). The region encoding the β7 ectodomain (M1-H723 ...
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bioRxiv - Cancer Biology 2023Quote: ... HLA and neoantigen transduction: Constructs expressing HLA-A*02:01 were linearized and restricted with BamHI and XhoI (New England Biolabs) and purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research Cat ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Immunology 2021Quote: ... The fragments were assembled using Hi-Fi DNA Assembly Mix (NEB). For the DonorgRNA vector ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: His-MBP-Fbp17SH3 was precipitated by amylose beads (NEB; cat#E8021L). After purification ...
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bioRxiv - Genetics 2023Quote: ... To facilitate homology directed assembly (Gibson or NEB Hi Fi assembly), 30bp homology with the preferred SEED cassettes were included at each site of the BsaI sites ...
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bioRxiv - Synthetic Biology 2023Quote: ... For his purpose pEKEx2 was first linearized using SacI (NEB, USA). The gene for mCherry was amplified by PCR using the primer pairs low_mCherry_fw and low_mCherry_rev (Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...
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bioRxiv - Bioengineering 2020Quote: A 10x Cas12-gRNA complex mixture was prepared by pre-incubating 1 μM of LbCas12a (NEB) with 1.25 μM gRNA in 1x NEBuffer 2.1 at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by permeabilization in PBS containing 0.5% Triton X-100 and ribonucleosidevanadyl complex (New England Biolabs) on ice for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: The coding sequence of the BTB domain of selected ZBTB family proteins was amplified from cDNA derived from the human HCT116 cell line using Q5 High-Fidelity DNA Polymerase (NEB). Specifically for the Patz1 construct ...
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bioRxiv - Biochemistry 2019Quote: Wild-type and mutant human FICD proteins (aa 104-445) were expressed as His6-Smt3 fusion constructs in T7 Express lysY/Iq (NEB) E ...
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bioRxiv - Genetics 2019Quote: ... Hi-C DNA was amplified using Index Primers set 1 (NEB, E7335S). The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... and the fragment was assembled using Hi-Fi DNA Assembly Mix (NEB) into the donor vector pADN171XS7 ...