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Citations for New England Biolabs :
1 - 50 of 1327 citations for HLA A*0201 GP100 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... phosphorylated using T4 PNK enzyme (#0201S NEB, Ipswich, MA USA), annealed and ligated into Esp3I-digested (#R0734L NEB ...
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bioRxiv - Developmental Biology 2021Quote: ... A pre-assembled complex of purified Cas9 protein (NEB) and gRNA was injected and the efficiency of Crispr/Cas9-induced mutagenesis in the F0 generation monitored at 24 hpf using a T7 endonuclease assay (Jao et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... an RNA-protein complex was formed with 1 µM Cas9 protein (New England Biolabs) and 1 µM gRNA pool (3 gRNAs in total ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: Ni pull down experiments were performed using 3μl of His tagged OsSRT1 constructs (0.3μg/ul) and 1μg recombinant human Histone H3 (NEB) and mixed with 20 μl of Ni-NTA slurry (Qiagen) ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2% vanadyl ribonuceloside complex (NEB)] and frozen at –80°C for at least 30 min ...
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bioRxiv - Biochemistry 2019Quote: ... 0.2% vanadyl ribonuceloside complex (NEB)] and frozen at –80°C for at least 30 min ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... RT-PCR was performed on cDNAs from murine Th17 or human HEK293 cells using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) with the standard thermocycling protocol ...
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bioRxiv - Molecular Biology 2022Quote: The HRAS minigene reporter was constructed by PCR amplifying a ∼900bp region spanning exon 4 to exon 6 from human genomic DNA isolated from HEK293 cells using Phusion high- fidelity DNA polymerase (NEB). The PCR fragment were inserted into pcDNA3.1(+ ...
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bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... the spacer-Trp2 sequence was cloned from pUC57 to downstream of MIP3α-gp100 and also to dMIP3α-gp1001using the XbaI restriction enzyme (New England Biolabs, Ipswich, MA) to create MIP3α-Gp100-Trp2 and dMIP3α-Gp100-Trp2 vaccines ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM vanadyl ribonucleoside complex (NEB), 0.02% RNase-free BSA (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... RVC (Ribonucleoside Vanadyl Complex, NEB, S1402) and ssDNA+ tRNA in 2× SSC ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM Ribonucleoside Vanadyl Complex (NEB), Roche cOmplete™ ...
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bioRxiv - Developmental Biology 2020Quote: ... 2 mM Vanadyl ribonucleoside complex (NEB), 0.02% RNAse-free BSA (ThermoFisher) ...
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bioRxiv - Systems Biology 2020Quote: ... 2mM Vanadyl ribonucleoside complex (NEB S1402S), and 0.1% Tween 20 (VWR 97062-332 ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mM ribonucleoside-vanadyl complex (NEB)) supplemented with 50 ng of Cy5-oligo-dT45 probe for 12 hr at 37°C in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM vanadyl ribonucleoside complex (NEB), 0.02% RNAse-free BSA (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Genomics 2022Quote: ... 10 mM ribonucleoside vanadyl complex (NEB). Dissociated tissue was filtered through a 70 uM cell strainer on ice and washed with an additional 5 mL of ice-cold lysis/fixation buffer ...
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bioRxiv - Genetics 2022Quote: ... 2mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM Ribonucleoside Vanadyl Complex (NEB), 0.2% v/v Triton X-100and 0.12 U/μL SUPERase•In™ RNase Inhibitor) ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM ribonucleoside vanadyl complex (NEB, S1402S), 2μg salmon sperm DNA (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and 0.2 mM vanadyl complex (NEB). 5 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Neuroscience 2023Quote: Codon-optimized human TDP-43-His LIC-A constructs were transformed into T7 express (New England Biolabs, #C3029J) E ...
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bioRxiv - Molecular Biology 2019Quote: ... After annealing the complex an equimolar amount was mixed with 1000ng Cas9 recombinant protein (NEB; final conc 20ng/ul) and incubated at RT for 15’ ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing CrtSPARTA protein complex was pooled and further loaded onto an amylose resin column (New England Biolabs). The column was washed with 20 column volumes (CV ...
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bioRxiv - Developmental Biology 2019Quote: ... and 2 mM ribonucleoside-vanadyl complex (NEB) for 30 minutes at 30ºC before overnight incubation with 10nM probes in hybridization buffer (10% (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ribonucleoside vanadyl complex (NEB S1402S), 100 units/mL SUPERase In (Thermo Fisher AM2694) ...
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bioRxiv - Microbiology 2021Quote: ... 2mM vanadylribonucleosid complex RNase inhibitor (NEB, USA)) ...
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bioRxiv - Biophysics 2022Quote: ... 2 mM ribonucleoside vanadyl complex (NEB, S1402S), 0.1% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 2mM vanadyl-ribonucleoside complex (New England Biolabs), and 10.6% dextran sulphate (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... Ribonucleoside vanadyl complex (New England Biolabs, S1402S). Formamide (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM ribonucleoside vanadyl complex (NEB, S1402S), 100 units/mL SUPERaseIn (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM vanadyl ribonucleoside complex (NEB, S1402S). The cells were then washed 4 times in wash buffer containing 25% formamide (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 10 mM ribonucleoside-vanadyl complex (NEB, S1402S)) and incubated overnight at 37°C in a humidified chamber ...
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bioRxiv - Molecular Biology 2024Quote: ... vanadyl ribonucleoside complex (VRC; New England Biolabs) was added to the NPB at a concentration of 10 mM ...
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bioRxiv - Cell Biology 2024Quote: ... + 2 mM vanadyl-ribonucleoside complex (NEB S1402) + 0.02% [w/v] BSA ...
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bioRxiv - Genetics 2024Quote: ... 2 mM Vanadyl Ribonucleoside complex (NEB S142), 0.5% BSA (Ambion ...
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bioRxiv - Plant Biology 2022Quote: ... His-TAD1 fusion proteins were purified according to the manufacturer’s protocol (New England Biolabs, Ipswich, MA, USA). The bait protein GST-WL1 was incubated with GST beads (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... and 5% (v/v) Ribonucleoside Vanadyl Complex (NEB)) at 4°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... and 5% (v/v) Ribonucleoside Vanadyl Complex (NEB)) and homogenized centrifuged at 15.000 g at 4°C.
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bioRxiv - Cell Biology 2019Quote: ... 2 mM ribonucleoside vanadyl complex (RVC) (NEB, S1402), 0.02% RNase-free BSA (Ambion ...
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bioRxiv - Systems Biology 2020Quote: ... and 5mM Ribonucleoside Vanadyl Complex (New England Biolabs). Cells and nuclei were then co-strained with 0.4μg.mL-1 green HCS CellMask (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... 10 mM ribonucleoside vanadyl complex (RVC by NEB), 33 mM 1,2-diheptanoylsn-glycero-3-phosphocholine (DHPC by Merck ...