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Citations for New England Biolabs :
101 - 150 of 2342 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... coli using standard transformation techniques with NEB 5-Alpha chemically competent cells (New England BioLabs), and to C ...
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bioRxiv - Synthetic Biology 2023Quote: All cloning was performed in NEB 5-alpha Competent Escherichia coli (New England BioLabs C2987U). The Pseudomonas putida KT2440 strain was purchased from ATCC (#47054) ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembled plasmid was introduced into Escherichia coli strain NEB 5-alpha (New England Biolabs) by heat shocking ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were amplified in chemically competent 5-alpha F’Iq Escherichia coli cells (New England Biolabs) after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: All plasmids were transformed into NEB 5-alpha Competent Escherichia coli cells (New England Biolabs) and purified using Qiaprep Spin Miniprep Kits or Plasmid Plus Maxi Kits for plasmid libraries (QIAGEN) ...
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bioRxiv - Biophysics 2024Quote: ... The ligation mix was then transformed into NEB 5-alpha competent cells (NEB, Cat. # C2987H) and colonies were selected using 50 µg/mL Kanamycin LB agar plate ...
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bioRxiv - Molecular Biology 2023Quote: ... the binding reaction was added to 200 μl of 5% (v/v) amylose beads (NEB) that has been blocked overnight with 1 mg/ml BSA and 0.5 mg/ml tRNA in 20 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Background assembly was measured by transformation of NEB 5-alpha competent cells (New England Biolabs C2987I) before cleanup of the assembly reactions using AmpureXP magnetic beads (Beckman Coulter A63880) ...
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bioRxiv - Genetics 2023Quote: ... All probes were 5’ end-labeled with [alpha-32P]-ATP and T4 polynucleotide kinase (NEB Inc.). The gels were exposed to a PhosphorImager ...
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bioRxiv - Immunology 2024Quote: ... the DNA was transformed into NEB® 5-alpha F’ I q chemically competent cells (NEB). Recombinants were selected on LB agar supplemented with 10 µg/ml of gentamicin ...
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bioRxiv - Molecular Biology 2024Quote: ... Cloning strains used were NEB Stable (lentiviral) and NEB 5-alpha (other plasmids) (New England Biolabs). For base editor cloning ...
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bioRxiv - Plant Biology 2024Quote: ... For molecular cloning and plasmid propagation Escherichia coli strain NEB® 5-alpha (New England Biolabs) and Stellar™ Competent Cells (Takara Bio Group ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2 μl of the assembly reaction was transformed into 5 μl chemically competent cells (E. coli NEB 5-alpha, New England Biolabs). Circuit constructs were analyzed by PCR ...
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bioRxiv - Biochemistry 2022Quote: ... Finale expression constructs were subcloned in NEB 5- alpha competent E.coli cells (New England Biolabs, Ipswich, US) followed by Sanger sequencing (Microsynth ...
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bioRxiv - Immunology 2021Quote: ... The assembled plasmid pCO2 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli NEB5-alpha (NEB#C2987) was used for the construction of all plasmids ...
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bioRxiv - Microbiology 2020Quote: ... Following KLD (kinase, ligase, and DpnI) treatment and transformation into NEB 5-alpha F’Iq cells (New England Biolabs), cells were grown as near-lawns on LB-chloramphenicol plates and a miniprep was performed from a resuspension of ~5000 colonies for each library.
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bioRxiv - Immunology 2020Quote: ... and the assembled plasmid pCO1 was transformed into NEB® 5-alpha competent Escherichia coli (High Efficiency) (NEB) according to manufacturer’s instructions (see Supplementary Figure 1 for plasmid maps) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli strain MET961 was constructed by replacing the glgB gene of strain NEB 5-alpha (New England Biolabs) with the glgB::Kan-pWV01repA region from E ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli NEB® 5-alpha was used for plasmid assembly and cloning following the manufacturer’s instructions (NEB). Transformed E ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H) and isolated using the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... Plasmids were transformed in 5’-Alpha or Stable competent bacteria (New England Biolabs, Cat C3040H and Cat C2987H), and extracted using a commercial kit (InvitrogenTM K182002) ...
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bioRxiv - Immunology 2023Quote: ... were single-cell sorted into 5 µl 1% (v/v) Nonidet P40 Substitute, Tris-HCl (20 mM, pH 8.0) containing 5 U murine RNase inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli NEB5-alpha (New England Biolabs) was diluted 1:100 in LB medium and grown to OD600 of 0.2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... NEB5-alpha (NEB cat no. C2987I) competent E ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was incubated at room temperature for 10 min followed by transformation into 5-alpha competent cells (NEB). The E ...
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bioRxiv - Microbiology 2021Quote: ... and npmA F and npmA_R, respectively (Table S5) Assembled vectors (Figs. S4C, S4D) were transformed into NEB 5-alpha (New England Biolabs), selecting for ampicillin and gentamicin resistance ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each library was split in two and transformed separately into 5-alpha cells (NEB, 3.5uL DNA in 35uL cells). Plasmid library isolation ...
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bioRxiv - Biochemistry 2024Quote: ... Purified RNA redissolved in 5 µl of 10% (v/v) DMSO was combined with 1x T4 RNA Ligase Buffer (New England Biolabs), 0.5 U/µl SuperaseIN (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... RNA dissolved in formamide was separated on 8% (w/v) acrylamide/7 M urea/1× TBE gels together with Low Range ssRNA Ladder (N0364S, New England Biolabs), stained with Diamond nucleic acid dye (H1181 ...
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bioRxiv - Biochemistry 2022Quote: ... The following cloning strains were used: NEB Stable (lentiviral and piggybac vectors) and NEB 5-alpha (all other plasmids) (New England Biolabs). For cloning of base editor constructs ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids carrying the dcas9/lacI cassette were recovered and propagated in the NEB 5-alpha F’ Iq strain (New England Biolabs). Cloning and/or propagation of plasmids containing the dcas9/lacI cassette in host E ...
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bioRxiv - Molecular Biology 2021Quote: ... coli NEB5-alpha cells (New England BioLabs) for quantitative exposure assays.
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bioRxiv - Microbiology 2022Quote: Chemically competent NEB5-alpha cells (NEB, C2987H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective LB agar plates (BD ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were loaded on to 1% (w/v) agarose gels stained with Midori Green Advance DNA stain (Geneflow #S6-0022) alongside 100 bp ladder (NEB #N3231S), before submitting for DNA Sanger sequencing (Source Genomics ...
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bioRxiv - Cancer Biology 2024Quote: ... Purified DNA fragments were cloned into pcDNA3.1 vector and transformed into 5-alpha competent cells (#C2987H, New England Biolabs, Ipswich, MA) and 10 colonies were picked for each cell line ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the resulting cell pellet was resuspended in 1 mL of RNA Protection Buffer (1× NEB DNA/RNA Protection Reagent, 1% (w/v) polyvinylpyrrolidone-40 ...
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bioRxiv - Synthetic Biology 2023Quote: Plasmids were transformed into BL21-alpha cells (NEB) and plated overnight at 37 □C on kanamycin LB plates (50 μg/mL) ...
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bioRxiv - Cell Biology 2022Quote: ... and transformed into competent DH5a E. coli (5-alpha Competent E. coli) following the protocol provided by the company (New England Biolabs, Ipswich, MA). For colony selection ...
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bioRxiv - Cell Biology 2021Quote: ... 1 uL of the Gibson Assembly reaction mixture was transformed via heat shock into chemically competent NEB 5-alpha cells (NEB, Catalog #C2988J), and cells were incubated on lysogeny broth with 10 μg/mL tetracycline (LB-Tet10 ...
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bioRxiv - Synthetic Biology 2021Quote: ... or dam+/dcm+ strain DH5-alpha Turbo (NEB C2984) to produce unmethylated or methylated plasmids ...
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bioRxiv - Plant Biology 2022Quote: ... 250 μL 2× lysis buffer (20 mM Tris-HCl, 40 mM Na2EDTA, 1% (w/v) SDS) and 3 μL proteinase K (NEB: P8102S, 20 mg/mL) were added and incubated under agitation at 55°C for 2 h ...
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bioRxiv - Developmental Biology 2020Quote: ... 1% v/v vanadyl ribonucleoside (NEB S14025), 0.2mg/mL BSA ...
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bioRxiv - Immunology 2022Quote: ... 2.5% v/v murine RNase inhibitor (NEB), pH 7.0) ...
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bioRxiv - Systems Biology 2023Quote: ... 1% (v/v) RNase inhibitor (NEB, M0314S) and 10% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... T4 DNA ligase and chemically competent DH5 alpha cells (NEB); Alt-R CRISPR-Cas9 crRNA ...