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Citations for New England Biolabs :
1401 - 1450 of 1706 citations for Bis 3 acetyl 4 hydroxyphenyl methane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... n=16 ligations were performed using 300 ng of digested and dephosphorylated Trono-BR backbone and 3 ng of digested insert with high concentration T4 DNA Ligase (NEB #M0202M). The ligation reactions were precipitated using QuantaBio 5PRIME Phase Lock Gel tubes before being resuspended in 3 µL of EB Buffer per 4 precipitated reactions ...
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bioRxiv - Systems Biology 2023Quote: ... 3) the wt bZIP sequences by digesting them out of them original plasmids with BamHI-HF (New England Biolabs, Ipswitch, MA) and SpeI-HF (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... and PE 2.0 (5′-CAA GCA GAA GAC GGC ATA CGA GAT CGG TCT CGG CAT TCC TGC TGA ACC GCT CTT CCG ATC* T-3′) for 15 cycles using Phusion polymerase (NEB M0530S). The library was purified by electrophoresis on a 1.2% agarose gel to get rid of adapter dimers ...
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bioRxiv - Microbiology 2023Quote: ... the gRNA cassette carrying the human U6 promoter and the invariant scaffold sgRNA sequence was inserted into the HIV-1 NL4-3 and HIV-1 CH077 pro-viral DNA between separated Nef and 3’LTR region using homologous recombination (NEB builder Hifi DNA assembly mastermix, NEB #E2621). The U6 promotor and the invariant scaffold are separated by a unique BsmBI restriction site using Q5® Site-Directed Mutagenesis Kit (NEB #E0554) ...
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bioRxiv - Cancer Biology 2023Quote: ... McGill University) and used to replace the mTurquoise of constructed 14-3-3ζ-mTurquoise using AgeI and NotI-HF (NEB; # R3189S). To conjugate Rluc8 to the N-termini of 14-3-3ζ ...
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bioRxiv - Genomics 2023Quote: ... Eluted RNA was treated with T4 PNK and preadenylated linker was ligated to the 3’ end using T4 RNA Ligase 2 truncated KQ (NEB, M0373L).
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bioRxiv - Cell Biology 2022Quote: ... TSF-ATG101:ATG13 (1-197) complex (final 3 μM) were incubated with 30 μl Amylose resin (New England Biolabs, Ipswich, MA) at 4 °C overnight in the ITC buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were washed 3 times with PBS and permeabilized 5 min on ice with permeabilize sol (1xPBS, 1%RNAse inhibitor Ribovanadylcomplex (RVC, NEB,#S1402S), 0,5 % Triton X-100 (Sigma ...
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bioRxiv - Genomics 2022Quote: ... blunt DNA fragments on the beads were adenine-tailed by adding 7μl of Klenow 3’→5’ exo-polymerase 5U/μl (New England Biolabs cat. #M0212L), 2.3μl of dATP 10mM and 5 μl NEB2 of 10x NEBuffer 2 and incubating the mixture 30 minutes at 37ºC and a further 10 minutes at 65ºC to inactivate the enzyme.
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bioRxiv - Genomics 2022Quote: ... The RNA was removed from beads by Trizol and then followed with the 3’ adapter ligation using T4 RNA Ligase 1 (NEB M0204L). After this ...
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bioRxiv - Genomics 2022Quote: ... the SapI sgRNA expression cassette was ligated into the KpnI linearized PX458 in a 3:1 molarity ratio using T4 DNA-ligase (New England Biolabs, M0202S) according to the manufacturer’s instructions followed by transformation and Sanger sequencing to verify successful cloning ...
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bioRxiv - Genomics 2022Quote: ... PX458 and the synthetized SapI sgRNA expression cassette (IDT, find sequence in Table 3) were digested with KpnI (New England Biolabs, R3142S). Next ...
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bioRxiv - Microbiology 2022Quote: ... Assembly of the two cDNA fragments was done using five overlapping cDNA fragments containing the VOC lineage defining mutations and replicon specific gene replacements (see Supplementary Table 3) using a NEBuilder® HiFi DNA Assembly Master Mix (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... The resulting double-stranded oligos had 5’ extensions that were complementary to the non-palindromic 3’ overhangs generated by BsaI-HFv2 (NEB, R3733) digestion of pJJW101 ...
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bioRxiv - Genomics 2022Quote: ... Approximately 3 μg of input DNA was dephosphorylated with alkaline phosphatase (ONT, cat SQK-CS9109) in CutSmart Buffer (NEB, cat B7204). Following enzyme inactivation of alkaline phosphatase ...
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bioRxiv - Cell Biology 2022Quote: ... stop codon and 3’ T7 terminator were used as templates for the coupled in vitro transcription/translation PURExpress system (New England Biolabs, USA). The various SQS constructs used for cysteine crosslinking comprised an N-terminal 3xFLAG tag ...
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bioRxiv - Microbiology 2023Quote: ... The 2X FLAG sequence was introduced via PCR as an oligonucleotide primer along with a reverse primer that produced the 3’ CNA1 UTR and cloned by use of the Gibson Assembly Cloning Kit (NEB #E5510S). The identity of the vector pCnat-CNA1-2X FLAG was also confirmed by sequencing.
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bioRxiv - Genomics 2024Quote: ... DNA and cDNA from the three replicates were kept separate and underwent a 3-cycle PCR using NEBNext Ultra II Q5 Master Mix (New England Biolabs, M0544L), forward primer P7-pLSmp-ass16UMI-gfp and reverse primer P5-pLSmP-5bc-i# ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was incubated for 3 days at 37C in the dark for conjugation and purified for 3 rounds using Monarch PCR & DNA Cleanup Kit (5 µg) (NEB, T1030S) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The transgene and sex of the F0 and F1 pups were determined by 3 PCRs of the Y chromosome using oligonucleotides described in Table S2 and LongAmp DNA polymerase (New England Biolabs, USA). Thermocycling conditions were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Small RNAs were treated with 5’ RNA polyphosphatase (Epicenter RP8092H) and ligated to 3’ pre-adenylated adapters with Truncated T4 RNA ligase (NEB M0373L). Small RNAs were then hybridized to the reverse transcription primer ...
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bioRxiv - Neuroscience 2024Quote: ... N-glycans were released from lyophilized glycopeptides after resuspension in 200 μL of 50 mM ammonium bicarbonate and incubated with 3 μL PNGase F (New England Biolabs, #P0704) at 37 °C for 4 h ...
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bioRxiv - Genomics 2024Quote: ... The RNA pellet was re-diluted and barcoded 3’-adapters ligated over night at 25°C using T4 RNA Ligase I (NEB, M0204L) in 10 µl reaction volume and 5 µM final 3’-adapter concentration ...
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bioRxiv - Systems Biology 2024Quote: ... but the 3’ SIRLOIN oligo was replaced with a duplex oligo ligated into the digested product with Hi-T4 ligase (NEB #M2622) at RT for 1 hr ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of digested psvIII vector was incubated with a 3 fold molar excess of PCR insert in 1x NEBuilder® HiFi DNA Assembly Master Mix (NEB) for 2 hours at 50°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Single turnover reactions were carried out as follows: 5 µM Clr4 was preincubated 5 minutes with 1 mM final S-adenosyl-methionine (liquid SAM, 3 2mM, NEB #B9003S), and varying concentrations of pSwi6 or unP Swi6 ...
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bioRxiv - Synthetic Biology 2024Quote: ... for RNA purification and recovery instead of AMPure XP beads and the use of heat-denaturation to release streptavidin-bound 3’-Desthiobiotin-GTP (New England Biolabs, N0761) capped RNA ...
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bioRxiv - Biochemistry 2024Quote: The library was prepared following our previously reported procedure with slight changes.[21] 3′-End repair and 5′-phosphorylation were conducted with T4 polynucleotide kinase (PNK) (NEB, M0201S). 16 µL RNA was mixed with 2 µL 10× T4 PNK reaction buffer and 1 µL T4 PNK ...
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bioRxiv - Microbiology 2024Quote: ... N-4005, and N-4004) were used to cap available 3’-terminal ends using terminal transferase (1 µl, 20 units) (NEB #M0315S), TdT reaction buffer (1.5 µl) ...
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bioRxiv - Molecular Biology 2024Quote: Small RNA sequencing libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following the manufacturer’s recommendations with exclusion of the initial size selection and the 3’ ligation step changed to 16°C for 18 hours to improve capture of methylated small RNAs (New England Biolabs, E7300S). Small RNA PCR products were size selected on a 10% polyacrylamide gel ...
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bioRxiv - Genomics 2024Quote: ... and 1.0 µL T4 DNA Ligase (100 units/µL, NEB M0202L buffer diluted 1:3 in NEB B8001S enzyme dilution buffer) were added to each reaction ...
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bioRxiv - Genomics 2024Quote: ... The aliquots were then divided into 3 digest reactions of 5 M cells and digested with the NlaIII restriction enzyme (NEB, R0125L). After subsequent proximity ligation and DNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... 5’UTR - GluA1flip(R) - 3’UTR and GluA3 flip(Q) (Y454A/R461G) was obtained using the Q5® Site-Directed Mutagenesis Kit (NEB). To obtain Halo-tag - GluA2flip(Q ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP–seq libraries were prepared from 3–5 ng of ChIPed DNA using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645S), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... cleanup was performed before dephosphorylation of ends followed by second digestion with the inner guides M2 and P2 (24 μL eluate, 3 μL Quick CIP (NEB, M0525S), and 3 μL 10X rCutSmart buffer (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Gels were sealed in a plastic bag and hybridized at 37 °C overnight with a 5′-(CCCTAA)5−3′ telomeric oligonucleotide probe 5′-end-labeled with T4 polynucleotide kinase (NEB M0201S) and [γ-32P]ATP (PerkinElmer BLU502A) ...
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bioRxiv - Biochemistry 2024Quote: ... where the biGBac compatible homology regions for Gibson Assembly (sequences A to F [14]; Supplementary Table 3) were inserted by BsmBI linearisation and HiFi DNA assembly (NEB, #E2621L) using ssDNA templates.
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bioRxiv - Cancer Biology 2024Quote: ... for 15 min at RT with 3 µl of the USER adaptor (NEBNext Multiplex Oligos for Illumina, NEB, cat. no. E7338A) for an additional 15 min at 37°C ...
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bioRxiv - Genetics 2024Quote: ... 5 µg of input gDNA in 24 µL nuclease-free water were dephosphorylated by incubation with 3 µL Quick CIP Phosphatase (NEB, M0525) and 3 µL rCutSmart™ Buffer at 37°C for 20 min (adapted from the original protocol ...
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bioRxiv - Cell Biology 2024Quote: ... were then ligated to pre-annealed Nano-tRNAseq 5’ and 3’ RNA adapters at room temperature for 2 hours with 20% PEG PEG 8000 (NEB, B10048) using recombinant E ...
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bioRxiv - Genetics 2021Quote: ... 2nd cDNA strand was again synthesized using a transcript specific primer (either 5’UTR_βG-intron _F or 5’UTR_CMV _F2, Table 4) and Q5 2x master mix (NEB, #M0494). After another round of bead purification ...
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bioRxiv - Genomics 2022Quote: To digest linear DNA 1 μg of DNA sample was incubated in 50 μl with 1× NEBuffer 4 (NEB), 1mM ATP (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were centrifuged at 30,597xg and 4°C for 20min and the supernatant was applied to amylose resin (NEB). The column was washed with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2020Quote: ... Vector and fragment were ligated in an overnight reaction at 4 °C using T4 DNA Ligase (New England Biolabs). After transformation ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 58,000 × g for 50 minutes at 4°C and the protein was batch purified using chitin beads (NEB). Protein-bound chitin beads were washed with lysis buffer and high salt buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The region of interest was amplified with specific primers (see Supplemental Table 4) and the Q5 DNA polymerase (NEB), and indexed by PCR using primers containing Illumina indexes (see Supplemental Table 4) ...
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bioRxiv - Biophysics 2020Quote: ... and loaded denatured (70 °C, 10 minutes) samples alongside an RNA ladder (2-4 μL, ssRNA ladder, N0362S, NEB). The resulting gels were stained with ethidium bromide for 30 minutes (0.5 μg/mL ddH20 ...
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bioRxiv - Biophysics 2020Quote: ... T270 plasmid was digested by HpaI at 37°C for 4 hr in the CutSmart buffer (New England BioLabs) to place the (TTAGGG)270 at the middle region of the linearized substrate ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2022Quote: ... Next, 500 nL of MspJI digestion mix (1x NEBuffer 4, 8x enzyme activator solution, 0.1 U MspJI (NEB, R0661L)) was added to each well and the plates were incubated at 37°C for 4.5 hours ...