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Citations for New England Biolabs :
1351 - 1400 of 10000+ citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... after PCR amplification and Gibson cloning (New England BioLabs). Constructs were confirmed by DNA sequencing and introduced into BL21 (DE3 ...
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bioRxiv - Microbiology 2023Quote: ... PCR reactions were performed using Q5 DNA polymerase (NEB) or PrimeSTAR MAX (Takara) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were purified by gel extraction (NEB, T1020) and sequenced on a NextSeq 500 (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... Diagnostic PCRs were performed with OneTaq DNA polymerase (NEB) or GoTaq DNA polymerase (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... A Q5 high fidelity PCR reaction (New England Biolabs) was performed on the cDNA to amplify TsKSRP and TsAGO genes using primers encoding a c-myc tag at the 3’ end ...
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bioRxiv - Evolutionary Biology 2023Quote: ... indexing PCR was performed using Q5 (New England Biolabs) under the following conditions ...
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bioRxiv - Cell Biology 2023Quote: ... New vectors were generated by PCR (Phusion-HF, NEB) into parent vectors digested with FseI/AscI unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... PCRs were performed using Phusion polymerase (New England Biolabs). Details on primer sequences ...
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bioRxiv - Biophysics 2023Quote: ... PCR products were digested with BamHI and NotI (NEB) and ligated into BamHI/NotI-digested pACYC-Duet-1 vector (Novagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed with Taq polymerase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... All PCRs were made using Q5 DNA polymerase (NEB). All constructs were sequence verified ...
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bioRxiv - Neuroscience 2023Quote: ... End point PCR analysis (Taq 2X Master Mix, NEB) was performed using 300 ng DNA template and 2-LTR circle primers ...
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bioRxiv - Neuroscience 2023Quote: ... were amplified by PCR employing Q5 polymerase (NEB#M0494). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... The oligo was obtained from IDT DNA and amplified by PCR using Phusion Polymerase (NEB F630) and purified for in vitro transcription using the MEGAshortscript T7 kit (Invitrogen AM1333) ...
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bioRxiv - Immunology 2023Quote: ... 25μl NEBNext High-Fidelity 2x PCR Master Mix (NEB), 2.5μl Nextera XT V2 i5 primer and 2.5μl Nextera XT V2 i7 primer (Illumina) ...
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bioRxiv - Microbiology 2023Quote: ... Each gene was PCR-amplified (Phusion; New England Biolabs) along with ∼300 bases 5’ of the translation start site to include the native promoter using primers designed with 16- 20 bases complementary to the gene of interest and 18-22 bases that overlap the NcoI site in pMV306hyg (Table S4) ...
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bioRxiv - Bioengineering 2024Quote: ... primarily using PCRs (Q5 ultra II HotStart Polymerase, NEB), Gibson Assemblies (NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... After 5 PCR cycles using Q5 polymerase (NEB #M0491) with 5X GC Buffer with a melting temperature of 72°C ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: ... All PCRs were conducted using Q5 DNA polymerase (NEB). Primer sequences are listed in the supplemental data (DataSet1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and PCR-amplified 2.5 mM DNTPs (NEB, CAT#N0447S), 10X PCR buffer (Klentaq ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting PCR products were treated with DpnI (NEB) to remove the template plasmid DNA and then self-ligated using Ligation High Ver2 (Takara) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was digested by EagI (New England Biolabs) and ligated into FMR1 RNA construct backbone instead of 5’UTR of FMR1 sequence using T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were circularized via ligation (New England Biolabs). For Melt-effector fusions ...
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bioRxiv - Immunology 2024Quote: ... we amplified the targeted gene regions by PCR (NEB Next High-Fidelity 2xPCR Master Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... The PCR product was cloned with BamHI (R0136S, NEB), NheI (R3131S ...
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bioRxiv - Biochemistry 2024Quote: ... A colony PCR using Taq Polymerase (New England Biolabs) on positive resistant colonies can help identifying false positives for Ampicillin resistance ...
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bioRxiv - Genomics 2024Quote: ... and NEBNext High-Fidelity 2x PCR Master Mix (NEB) for 12 cycles to generate each library ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were subsequently ligated using T4 ligase (NEB) in a reaction with DpnI (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting PCR product was treated with DpnI (NEB), gel purified using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were performed using the Q5 DNA polymerase (NEB) in a GeneAmp PCR system 2400 (Applied Biosystems).
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bioRxiv - Microbiology 2019Quote: ... All PCR reactions were carried out with Phusion® High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, MA, USA) and high-fidelity polymerase (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with forward primer “reverse transcription primer (RTP)” ...
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bioRxiv - Plant Biology 2021Quote: The first round of PCR was performed using 1X Phusion High-Fidelity PCR Master Mix with HF Buffer (New England BioLabs; Ipswitch, MA, USA) with cDNA diluted 1:10 in water ...
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bioRxiv - Genomics 2023Quote: ... Amplification of the libraries was performed for 13 PCR cycles using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, cat. no. M0531L); 6-bp molecular barcodes were also incorporated during this PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... Amplification was achieved through 16S rRNA gene Polymerase Chain Reaction (PCR) with Illumina (San Diego, USA) adapted primers [50] and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, USA) at 98°C (2 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... PCR with custom Nextera PCR primers was then performed for five cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs, Ipswich, MA, USA) 76 ...
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bioRxiv - Molecular Biology 2021Quote: Pooled sgRNA oligonucleotides were synthesized as 76-mers by Custom Array (Bothell, WA, USA) and were amplified by PCR with NEBNext High Fidelity PCR Master Mix (New England BioLabs, NEB, Ipswich, MA, USA) using customized primers (Additional file 2 ...
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bioRxiv - Cell Biology 2022Quote: ... The V3-V4 hypervariable regions of bacterial 16S rRNA genes were amplified by PCR with Phusion® High-Fidelity PCR MasterMix (New England Biolabs, Ipswich, MA, USA) using specific primer as previously described59 ...
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bioRxiv - Molecular Biology 2019Quote: ... The templates of dsRNA were initially amplified from the first-strand cDNA and secondary amplified from the initial PCR products by RT-PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs Japan Inc., Tokyo, Japan). The initial PCR primers are described as Hvig_diap1_F/Hvig_diap1_R ...
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bioRxiv - Genomics 2021Quote: ... and 7 PCR cycles were used to amplified libraries using NEBnext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich MA, Catalog #M0541S) and SYBR Green I (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... Almost full-length PCR products were obtained in a preliminary PCR using 1.25U OneTaq® Quick-Load® DNA Polymerase (New England BioLabs, Ipswich, MA, USA), 200 μM mixed dNTPs ...
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bioRxiv - Microbiology 2024Quote: ... 5’-GCT GCG TTC TTC ATC GAT GC-3’) were chosen with barcodes for PCR amplification using Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, USA). The PCR products were then mixed at equal density ratios and purified with a Gel extraction kit (QIAGEN ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were cloned into pMiniT (New England Biolabs), and the stop codon was altered to create a NheI site using site-directed mutagenesis ...
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bioRxiv - Synthetic Biology 2021Quote: ... All plasmid-template PCR products were treated with DpnI (NEB) following standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR amplified (8-12 cycles) with Phusion polymerase (NEB) using custom primers (22) ...
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bioRxiv - Genetics 2020Quote: ... We performed this PCR using Taq DNA Polymerase (M0320S, NEB) and the following conditions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase (NEB). Each reaction contained 1x Q5 reaction buffer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1X Phusion High Fidelity PCR Mastermix (New England Biolabs). Cycle conditions were 98°C for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA polymerase (NEB) while qPCR products were amplified using Ssofast EvaGreen Supermix (BioRad ...