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Citations for New England Biolabs :
1301 - 1350 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... which was injected into one-cell state embryos together with Cas9 protein (New England Biolabs). Mutant animals were identified by PCR using the primers ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... were precleared using 40μl of Protein-A Magnetic beads (New England Biolabs Cat No-S1425S) to remove non-specific components from the lysate ...
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bioRxiv - Microbiology 2021Quote: ... The protein expression level was evaluated in four T7 expression strains: T7 Express (C2566, NEB), T7 Express with LysY and lacIq (C3013 ...
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bioRxiv - Molecular Biology 2021Quote: ... IgG (Epredia™, NC748P) or 1X PBS was incubated with protein A beads (NEB, S1425S) with agitation for 1 hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... to visualize the bands alongside a color prestained protein broad range standard (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: Injection mixes for CRISPR mediated manipulations consisted of 650 ng/ul dCas9 protein (NEB, M0652) or Cas9 protein (NEB ...
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bioRxiv - Genetics 2019Quote: ... sgRNAs were mixed with Cas9 protein (gift from Darren Gilmour, M0646M NEB, or B25641 invitrogen) and co-injected into 1-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μg of microsomal proteins were treated with EndoH-Hf (New England Biolabs, Ipswich, MA) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were formed from sgRNA and recombinant Cas9 2NLS protein (New England Biolabs) mixed at a sgRNA to Cas9 ratio of 4.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lambda protein phosphatase procedure was carried out following the manufacturer’s guidelines (New England Biolabs). The sample was incubated at 30°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Bioengineering 2023Quote: The heterologous protein MBPeGFP was purified using NEBExpress Ni spin columns (NEB, Ipswich, MA, USA) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Chromatin was pre-cleared with 20 μl Protein A magnetic beads (New England Biolabs, S1425S) for 1.5 h and 100 μL of the solution was saved as an input control ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 1x NEBuffer for Protein MetalloPhosphatases (New England BioLabs; supplied with λPP), with 0.5% NP-40 and 0.01 mg/mL leupeptin ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein products were purified using the NEBExpress Ni Spin Columns (New England Biolabs, S1427S). The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
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bioRxiv - Molecular Biology 2023Quote: RNA-protein complexes were isolated with oligo[dT)]25 magnetic beads (New England Biolabs, S1419S). Briefly ...
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bioRxiv - Immunology 2023Quote: ... For embryo microinjection the annealed sgRNA were each complexed with Cas9 protein (New England Biolabs) at room temperature for 10min in injection buffer (final concentrations ...
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bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
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bioRxiv - Bioengineering 2023Quote: ... The luciferase protein expressed in NiCo21(DE3) competent Escherichia coli (New England Biolabs, MA, USA) was purified using a Ni-NTA agarose resin column (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... All aliquots were supplemented with 10X NEBuffer for Protein MetalloPhosphatases (PMP) and 10X MnCl2 (NEB). The first aliquot (Untreated control ...
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bioRxiv - Physiology 2024Quote: ... 20 mg of protein from each sample was subjected to PNGase F treatment (NEB #P0704) to remove N-linked oligosaccharides prior to SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Biochemistry 2023Quote: ... protein was combined with 1.5x molar excess of SNAP-Surface Alexa488 dye (NEB, Cat# S9129S). SNAP dye labeling was performed in buffer containing 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for 5 µg trimer ...
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bioRxiv - Developmental Biology 2024Quote: ... adrb1-/- zebrafish was obtained by coninjection of Cas9 protein (EnGen Spy Cas9 NLS, M0646, NEB) with gRNA targeting adrb1 ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA molecules were purified using a commercially available purification kit (MonarchQR Plasmid Miniprep Kit, NEB). The purified plasmids were run on a 0.8% agarose gel supplemented with 2.5 µg/mL of chloroquine in 1xTBE buffer containing 2.5 µg/mL of chloroquine at 25 V for 15 hours ...
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bioRxiv - Plant Biology 2020Quote: ... using the Gibson assembly kit (NEB). The pET28b vector was linearized by digesting with BamHI and XhoI to create overhangs compatible for Gibson assembly ...
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bioRxiv - Biophysics 2020Quote: Using PURExpress kits (New England Biolabs), 37.5 μL IVT reactions were assembled on ice with 1.5 μl RNase inhibitor ...
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bioRxiv - Microbiology 2020Quote: ... using the NEBuilder HiFi kit (NEB).
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bioRxiv - Microbiology 2020Quote: ... using the NEBuilder HiFi kit (NEB). The mdtU-lacZ translational fusions were constructed by PCR-amplifying the mdtU gene from either a WT (E ...
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bioRxiv - Molecular Biology 2022Quote: ... NEBNext rRNA Depletion kit( NEB #E7405) was used to deplete ribosomal RNA ...
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bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
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bioRxiv - Cell Biology 2019Quote: ... using Gibson assembly cloning kit (NEB). COX8A-SNAP vector was commercially obtained from NEB ...
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bioRxiv - Genomics 2021Quote: ... NEBNext UltraII Q5 kit (NEB M0544) was used for PCR enrichment ...
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bioRxiv - Microbiology 2021Quote: ... using Monarch Plasmid Miniprep kit (NEB). Sequencing was through the company Genewiz.
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bioRxiv - Molecular Biology 2021Quote: ... Q5 Site-Directed Mutagenesis Kit (NEB) was used for generating dCas9 plasmids encoding the mutant p300core* (Y1467F ...
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bioRxiv - Immunology 2022Quote: ... The second strand synthesis kit (NEB) at 16°C for 2 hours was used for cDNA synthesis followed by a 1.4X AMPure XP bead cleanup ...
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bioRxiv - Cell Biology 2022Quote: ... Q5 site directed mutagenesis kit (NEB) was used and the manufacturer’s instructions were followed.
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bioRxiv - Biophysics 2020Quote: ... and Q5 Hotstart Mutatagenesis kit (NEB) were used.
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bioRxiv - Genomics 2022Quote: Monarch Genomic DNA Purification kit (NEB)
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bioRxiv - Developmental Biology 2022Quote: ... “Q5 Site-Directed Mutagenesis Kit” (NEB) was used ...