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Citations for New England Biolabs :
1201 - 1250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... dCas9-SNAP protein samples were incubated with SNAP-Surface Alexa Fluor 647 (NEB S9136) at 37°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... The proteins were expressed in BL21 DE3 competent cells (New England Biolabs, Ipswich, MA). Protein purification was undertaken using nickel-affinity chromatography (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... Recombinant proteins were purified using gravity flow columns with amylose resin (New England Biolabs). MBP cleavage was performed by incubation in cleavage buffer (50 mM Trizma-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... Precipitated proteins were resuspended in 150 μL of Glycoprotein Denaturing Buffer (New England Biolabs) and incubated at 100 °C for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... reduced and alkylated and proteins were digested with first PNGase F (New England BioLabs), endoproteinase Lys-C (Promega ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... against a Colour Prestained Protein Standard (Broad Range 10-250 kDa, New England BioLabs) and resolved by electrophoresis for 70 minutes at 150-180V ...
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bioRxiv - Neuroscience 2020Quote: ... AP fusion proteins are quantified using calf intestinal alkaline phosphatase (CIP, New England Biolabs) activity ...
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bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in phosphatase buffer with 200U lambda protein phosphatase (New England Biolabs) at 30 °C for 20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein sample was concentrated by ultrafiltration and incubated with PNGaseF (New England Biolabs) overnight.
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bioRxiv - Neuroscience 2022Quote: Recombinant proteins were labeled with SNAP-Surface 549 dye (New England BioLabs, Cat# S9112S) for visualization ...
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bioRxiv - Biophysics 2022Quote: ... purified proteins were labelled with SNAP-Surface Alexa Fluor 647 tag (New England Biolabs) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... The denatured proteins were then supplemented with 500 U of EndoH-MBP (EndoHf, NEB) and incubated for 45 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... All other proteins were expressed in Escherichia coli BL21(DE3) cells (New England BioLabs).
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bioRxiv - Cell Biology 2019Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was degraded by adding 50 µl of 20mg/ml proteinase K (NEB, P8102), 50 µl of 10% SDS and incubated at 55°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at RT for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2-bound proteins were eluted by adding 7.5 μl RNase H (NEB), 2 μl TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: Recombinant proteins from Escherichia coli lysates were immobilized on amylose resins (New England Biolabs), incubated for 1 h at 4 °C with purified GST-SH3P2 ...
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bioRxiv - Cell Biology 2019Quote: ... one was treated with 400U Lambda protein phosphatase (Lambda PP, New England Biolabs, #P0753S) in 50µl phosphatase assay buffer ...
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bioRxiv - Immunology 2020Quote: ... For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Proteins were then mixed with Buffer A supplemented with S-adenosyl-methionine (NEB B9003S) containing 1 ug of either naked unmethylated DNA (Plasmid ASP3552 ...
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bioRxiv - Cancer Biology 2022Quote: ... and biotin-labelled proteins were isolated by immunoprecipitation using streptavidin magnetic beads (NEB, S1420S) in an immunoprecipitation buffer containing 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of S proteins were digested respectively using alpha lytic protease (New England BioLabs), chymotrypsin (Athens Research and Technology) ...
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bioRxiv - Plant Biology 2022Quote: ... For recombinant protein expression we used GW versions of pMAL-C2 (New England Biolabs) and pDEST-17 (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... and protein purification was carried out according to the manual of Amylose Resin (NEB). The protein ...
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bioRxiv - Microbiology 2022Quote: ... The final injection mix contained 300 ng/μL spCas9 protein (M0386, New England Biolabs), 40 ng/μL of each sgRNA (3 targeting exon 5 and 7 of Dicer2 and 4 targeting the kmo gene ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
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bioRxiv - Molecular Biology 2022Quote: ... The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified on the amylose resin (New England Biolabs, Ipswich, MA, USA) and stored at −20 °C in 25 % glycerol ...
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bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs). The fractional dye labeling and DNA binding activity were assessed as previously described [12].
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bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Physiology 2023Quote: ... The proteins were then purified by affinity chromatography using amylose resin (Catalog E8021L, NEB). The amylose resin was washed and pre-equilibrated in 20 mM tris ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (NewEngland Biolabs) as a standard ...
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bioRxiv - Immunology 2023Quote: ... with a mixture of 0.33 µM EnGen Cas9 protein (New England Biolabs, Cat.No. M0646T), two Cas9 guides RNA ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
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bioRxiv - Immunology 2023Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified on amylose resin (New England Biolabs, Ipswich, MA, USA; Cat# E8021) and eluted with maltose using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of a broad range color pre-stained protein standard (New England BioLabs) was added to the outer lanes ...
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bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Plant Biology 2024Quote: ... The protein samples were treated with Endo Hf (New England BioLabs, Ipswich, MA, USA) at 37°C for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were then incubated with MBP amylose magnetic beads (New England Biolabs) for 1 h at 4°C and washed with 200 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: For lambda phosphatase assay the reactive from Lambda Protein Phosphatase (Lambda PP) (P0753L, NEB) were used and followed the protocol from the manufacturer.
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Molecular Biology 2024Quote: ... purified using column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with Nhe1 and BamH1 double-digested and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 ...