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Citations for New England Biolabs :
1251 - 1300 of 1934 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were recovered in 1 ml NEB® 10-beta/Stable Outgrowth Medium (NEB, B9035) at 37 °C for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: RNA from K562 cell lines was extracted using the Monarch Total RNA purification kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... following staining for PreS2-LHBS and autolysosome by SNAP-Cell® TMR-Star (S9105S, NEB) and DALGreen (D675-10 ...
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bioRxiv - Cell Biology 2022Quote: ... RAD18 knockout positive cells were confirmed with PCR using Q5 High Fidelity Master Mix (NEB), sanger sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... at least 1×106 fixed cells were digested using the DpnII restriction enzyme (NEB, R0543M). Chromatin was re-ligated with T4 ligase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, MA, USA), Iso-Seq Express Oligo Kit ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from cells using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... HHV6A BAC DNA was electroporated into JJhan T cells using Lonza (NEB, New Brunswick, MA). When the cells in the transfected culture reached 80% GFP+ ...
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bioRxiv - Developmental Biology 2020Quote: ... which was injected into one-cell state embryos together with Cas9 protein (New England Biolabs). Mutant animals were identified by PCR using the primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were labelled with SNAP-Surface-649 (0.5 µM, from New England Biolabs, Hitchin, UK) for 30 min at 37°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using standard transformation techniques with NEB 5-Alpha chemically competent cells (New England BioLabs), and to C ...
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bioRxiv - Genomics 2021Quote: ... the ligation mixes were heat-shock transformed into NEB Stable competent cells (New England Biolabs) and grown as single colonies on LB-Agar (supplemented with Ampicillin 100 μg/ml ...
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bioRxiv - Genomics 2021Quote: ... The ligation mixes were heat shock transformed into NEB Stable competent cells (New England Biolabs) and grown as single colonies on LB-Agar plates (supplemented with Ampicillin 100 ug/ml ...
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bioRxiv - Genetics 2020Quote: ... and then introduced into Escherichia coli DH5α competent cells (New England Biolabs, Ipswich, MA, USA). The resulting plasmid was integrated into a recipient S ...
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bioRxiv - Immunology 2021Quote: Phagmids encoding the sequences of the selected nanobodies were transformed into BL21 cells (NEB, USA) for expression and purification ...
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bioRxiv - Biochemistry 2021Quote: C-terminally (His)6-tagged LukE was expressed in BL21 (DE3) Escherichia coli cells (NEB). Transformed cells were grown at 37 °C in Terrific broth supplemented with 100 μg/mL ampicillin to a density of OD600 = 0.6 ...
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bioRxiv - Immunology 2020Quote: Heavy and light chain vectors were verified by sequencing and transformed into DH5alpha cells (NEB). The transformed cells were grown and the plasmid was purified by midiprep (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2020Quote: ... Denatured cell lysate was then deglycosylated using 500 U of PNGase F (New England Biolabs) in 1x glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Developmental Biology 2021Quote: ... The constructs were transformed into BL21(DE3) competent cells for expression (C2527, New England Biolabs).
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bioRxiv - Immunology 2022Quote: ... cells were digested with Micrococcal Nuclease at 37°C for 40 min (New England BioLabs) and added 10 mM EDTA to stop the reaction ...
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bioRxiv - Biochemistry 2022Quote: Bacterial expression vectors were transformed into Escherichia coli BL21 (DE3) competent cells (New England Biolabs). The bacterial cultures were grown in LB until they reached OD600nm 0.8 ...
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bioRxiv - Bioengineering 2022Quote: ... 200 Ω) and cells were recovered in 1 mL SOC medium (New England Biolabs, #B9020) at 30 °C and for 90 min ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of cell samples was extracted using the Monarch Total RNA kit (NEB, T2010S) and then reverse transcribed with iScript cDNA Synthesis kit (Biorad ...
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bioRxiv - Cell Biology 2022Quote: ... dynein coated beads were incubated with 5 μM SNAP-Cell TMR (New England BioLabs, #S9105S) in the column for 10 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... We used the product of Gibson assembly for transformation into DH5α competent cells (NEB: C2987H). Transformed cells were cultured directly in LB liquid ...
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bioRxiv - Neuroscience 2023Quote: ... and those involving AAV plasmids were transformed into NEB Stable competent cells (New England Biolabs).
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bioRxiv - Synthetic Biology 2022Quote: Total RNA was extracted from cells using the Monarch Total RNA Miniprep Kit (NEB #T2010), following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Total RNA was extracted from cells using the Monarch Total RNA Miniprep Kit (NEB #T2010), following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin in the cell lysate was digested using Microccocal nuclease (MNase, New England BioLabs, M0247S) at room temperature for 5 min and 5 ul 0.25 mM EDTA was added to stop the reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were then incubated with RNase H (NEB, 1: 50 in 1X RNase H buffer) at 37°C for 4 hours ...
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bioRxiv - Genomics 2024Quote: ... All arrayed cloning was performed in NEB Stable chemically competent cells (New England Biolabs C3040H) according to the manufacturer’s recommendations and all cultures were grown at 30 °C ...
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bioRxiv - Molecular Biology 2024Quote: The flow cell chamber was passivated with PBS-BSA buffer (1.0 mg/mL BSA; NEB) in PBS for 5 min ...
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bioRxiv - Bioengineering 2023Quote: ... Frozen cell pellets were resuspended in 4 mL of IMAC buffer (NEB, Ipswich, MA, USA) on ice and dispersed using an ultrasonic disruptor (Sonics ...
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bioRxiv - Biophysics 2024Quote: ... The resulting construct was expressed in Escherichia coli BL21(DE3) cells (New England Biolabs, C2527H) at 18 °C for 16 hours after induction with 0.2 mM isopropyl-β-D-thiogalactoside (Goldbio ...
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bioRxiv - Genomics 2022Quote: ... GP5d cell nuclei were isolated and treated with GC methylase M.CviPI (New England Biolabs, M0227) as described[61] ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... The barcode plasmid libraries were used to transform T7 Express chemically competent cells (NEB #C2566I) to establish barcoded E ...
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bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
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bioRxiv - Developmental Biology 2023Quote: ... consisting of: 8.3 µl Luna Cell Ready Lysis Buffer 2X (New England Biolabs, Ipswich, MA), 0.66 µl Luna Cell Ready RNA Protection Reagent 25X (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs) was used to generate cDNA from the extraction ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from subconfluent cells with the Monarch total RNA purification kit (NEB), and reverse transcribed with LunaScript RT SuperMix (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... Calmodulin and myosin-5 expression plasmids were prepared from E.coli 5α cells (New England Biolabs), transformed with the each of plasmids ...
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bioRxiv - Biochemistry 2023Quote: Infection in HCT116: Cells were infected with virus and 8 μg/mL polybrene (NEB, #H9268).
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bioRxiv - Cancer Biology 2023Quote: Equal number (3×106) of cells were harvested in ice-cold nuclear extraction buffer (NEB) (400 μl ...
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bioRxiv - Plant Biology 2023Quote: ... The ligation product was introduced into NEB 5α competent Escherichia coli cells (NEB, Evry, France), transformants selected on kanamycin (50 µg.mL-1 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were amplified in chemically competent 5-alpha F’Iq Escherichia coli cells (New England Biolabs) after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... plasmids were chemically transformed into DH5-alpha cells (cat no. C2987, New England Biolabs Inc.) and plated on agar plates containing appropriate antibiotics ...
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bioRxiv - Genomics 2023Quote: Cells were resuspended in 330 μl of ligation mix (1x T4 ligase buffer (NEB, B0202S), 100 Units T4 DNA ligase (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: AtPAXX fused to a hexahistidine tag (pHAT4-atpaxx) was expressed in BL21(DE3) cells (NEB). The proteins were purified using Ni-NTA (Qiagen) ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019), where colony PCR and Sanger sequencing were again used to determine if the clone was correct.