Labshake search
Citations for New England Biolabs :
1051 - 1100 of 1934 citations for Primary Human Coronary Artery Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... The cells were grown in 1 mL SOC Outgrowth Medium (B9020, NEB) for 2 h in a standard glass tube at 30 °C with 160 rpm ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were labelled with SNAP-Surface Alexa Fluor 546 (New England Biolabs) and SNAP-expressing fluorescent cells sorted by fluorescence-activated cell sorting (FACS ...
-
bioRxiv - Microbiology 2024Quote: Cell lysates were digested for 1 h with Endo H (P0702, NEB) or PNGase F (P0704 ...
-
bioRxiv - Microbiology 2024Quote: ... Assembled plasmid libraries were then electroporated into electrocompetent 10b cells (NEB C3020K) and incubated overnight on ampicillin containing LB plates ...
-
bioRxiv - Biochemistry 2024Quote: ... the corresponding plasmid was transformed into T7 express cells (New England Biolabs) and grown overnight at 37°C in 5 mL of LB-medium ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... transfected cells were labeled with SNAP-Surface Alexa Fluor 647 dye (NEB) as described earlier ...
-
bioRxiv - Cancer Biology 2024Quote: ... vectors were transformed into T7 Express lysY competent cells (New England Biolabs). For histone H4 expression ...
-
bioRxiv - Synthetic Biology 2024Quote: All DNA manipulations were performed using NEB Turbo cells (New England Biolabs) or Mach1F cells ...
-
bioRxiv - Synthetic Biology 2024Quote: ... All DNA manipulations were performed using NEB Turbo cells (New England Biolabs) or Mach1F cells ...
-
bioRxiv - Biophysics 2021Quote: ... This vector was transformed into T7 express cells (enhanced BL21 derivative, NEB C2566I), grown to OD∼0.4-0.8 ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were allowed to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 30 minutes and later transferred to 200 mL of LB medium with ampicillin 4X overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... the ligated products were transformed into NEB 5α competent cells (New England BioLabs) using the heat shock method ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then transfected with sgRNA and the EnGen Cas9 NLS protein (NEB) using Lipofectamine RNAiMAX and according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... washed before either incubation with SNAP-Cell TMR-Star at 2 μM (NEB) and SNAP-Surface Block at 13 μM (NEB ...
-
bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1.2 mL of 1X DpnII buffer (NEB, Ipswich, MA), transferred to a VK05 tubes (Precellys ...
-
bioRxiv - Genomics 2020Quote: ... and the resulting product was transformed into 10-Beta Electrocompetent cells (NEB C3020K). We plated 1% of the library to estimate complexity and grew the rest of the sample and then midi prepped (Zymo D4200 ...
-
bioRxiv - Neuroscience 2019Quote: ... was first transformed into NEB 5-alpha competent cells (New England Biolabs #E2621S) and plated on LB plus ampicillin (60 µl/ml).
-
Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... we used either a 647-SiR dye (SNAP-Cell® 647-SiR, NEB) or TMR-Star dye (SNAP-Cell® TMR-Star ...
-
bioRxiv - Systems Biology 2020Quote: ... cells were then resuspended in 600 μL 1x T4 ligase buffer (M0202L, NEB). The following additional reagents were added to make a master mix ...
-
bioRxiv - Immunology 2019Quote: ... Gel filtered proteins were labeled with either SNAP-Cell 505 (NEB, catalog S9103S) or SNAP-Cell TMR (NEB ...
-
bioRxiv - Microbiology 2019Quote: ... For live cell imaging parasites were stained with Hoechst 33342 (New England Biolabs), transferred to poly-L-lysine-coated μ-slides VI (Ibidi ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 µg cell lysate was treated with 80 units of lambda-phosphatase (NEB) at 30°C for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-glutaryl-lysine (Cell Signaling Technologies, non-commercial #14943MF and PTM-Biolabs #1151), anti-succinyl-lysine (Cell Signaling Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-succinyl-lysine (Cell Signaling Technologies, non-commercial # 13599 and PTM-Biolabs #401), anti-malonyl-lysine (Cell Signaling Technologies ...
-
bioRxiv - Biochemistry 2021Quote: Plasmid propagation and subcloning was performed using Escherichia coli DH10β competent cells (NEB). The codon optimized GTL gBlock (Supporting Information Table 1 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were lysed in 150μL of 1x Gel Loading Buffer (New England Biolabs), sonicated and boiled 5min at 95°C ...
-
bioRxiv - Biophysics 2021Quote: ... where GRN-3 was expressed in Escherichia coli SHuffle cells (New England Biolabs) while other GRNs were expressed in Origami 2 DE3 cells (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... The vector was transformed into BL21 (DE3) competent cells (New England Biolabs, UK) for expression ...
-
bioRxiv - Synthetic Biology 2021Quote: Entry and expression plasmids were grown in NEB-5α cells (New England Biolabs). Destination plasmids were grown in One Shot ccdB survival cells (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... The assembly reaction was then transformed into high efficiency DH5alpha cells (NEB C2987I). For eutD ...
-
bioRxiv - Synthetic Biology 2022Quote: ... These plasmids were transformed into chemically competent BL21(DE3) cells (New England Biolabs) and single transformants selected for using ampicillin resistance.
-
bioRxiv - Biochemistry 2022Quote: Cell lines and molecular biology materials were purchased from New England Biolabs (NEB) unless otherwise indicated ...
-
bioRxiv - Cancer Biology 2022Quote: ... The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB). To prepare the transposase ...
-
The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... cells were lysed in 150μL of 1x Gel Loading Buffer (New England Biolabs), sonicated and boiled 5min at 95°C ...
-
bioRxiv - Microbiology 2022Quote: ... All cloning and strains were performed in dh10b genotype cells (NEB, Intact Genomics).
-
bioRxiv - Cell Biology 2022Quote: ... Cells were labelled in full media with SNAP-Surface 549 (New England Biolabs) for 30 minutes at 37°C and imaged in RPMI without phenol red in a Nikon Eclipse Ti spinning disk confocal microscope with an ORCA-Flash 4.0 camera (Hamamatsu ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were preserved in a 1:3 glacial acetic acid: methanol (Biolabs-chemicals) solution and karyotyped using g-banding.
-
bioRxiv - Plant Biology 2021Quote: ... constructs were separately introduced into the Escherichia coli (Express Competent cells C2523H, NEB). The purification was performed using amylose resin (NEB ...
-
bioRxiv - Developmental Biology 2021Quote: ... and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB). Only embryos exhibiting low mosaic sfGFP expression were grown to adulthood ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were labelled with 1 μM SNAP-Surface 549 (New England Biolabs) for 15 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... and E-cadherin (24E10, Cell Signaling Technology, NEB, Frankfurt, Germany, #3195, 1:400) were used for immunohistochemistry (IHC ...
-
bioRxiv - Biochemistry 2021Quote: ... Transformation of the resulting construct into NiCo21(DE3) cells (New England Biolabs, USA) and culture in a ZY5052 medium (25 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The plasmid was amplified in DH5a cells (NewEngland Biolabs, Ipswich, MA; Cat #C2987H), and purified using plasmid purification midi-kit (Qiagen ...
-
bioRxiv - Molecular Biology 2022Quote: ... The ligation was directly transformed into NEB-5-alpha electrocompetent cells (NEB C2987H) and plated on LB-agar supplemented with carbenicillin (100 µg/mL) ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was extracted from cells using Monarch total RNA Miniprep kit (NEB). cDNA synthesis was performed using LunascriptTM RT Supermix Kit (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were stained with SNAP-Surface® 549 (DY549P1) (S9112S, New England Biolabs) for 15 min in a 37° incubator with 5%CO2 and sorted for the fluorescent signal.
-
bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S ...
-
bioRxiv - Molecular Biology 2019Quote: ... A total of 107 cells were cross-linked and digested with HindIII (NEB). After inactivating HindIII ...
-
bioRxiv - Cell Biology 2019Quote: ... The time taken for each cell to progress from nuclear envelope breakdown (NEB) to anaphase onset (chromatid separation ...
-
bioRxiv - Microbiology 2019Quote: ... Growth was monitored every 2 min using a Cell Growth Quantifier (Aquila Biolabs).