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Citations for New England Biolabs :
1201 - 1250 of 4533 citations for 5 2 3 Difluorophenyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... and 72°C for 5 min) (Phusion High-Fidelity DNA Polymerase, NEB, M0530S) using the P7 and a T7-fused P5 primer (5′ TAA TAC GAC TCA CTA TAG GGA ATG ATA CGG CGA CCA CCG A 3′ ...
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bioRxiv - Biochemistry 2023Quote: ... NEB Stable (lentiviral vectors) and NEB 5-alpha (other plasmids) (New England Biolabs) were used as cloning strains ...
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bioRxiv - Biochemistry 2024Quote: ... and then ligated to cDNA using Thermostable 5’ App DNA/RNA Ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5 mM BME) onto a 10 ml amylose column (New England Biolabs). The amylose column was washed with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using Monarch PCR & DNA Cleanup Kit (5 μg; NEB) and checked on 1% agarose gel and approximately 600 ng of each PCR product was used as template for in vitro dsRNA synthesis according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were mixed with Hydrophilic Streptavidin Magnetic Beads (5 µl) (NEB #S1421S) and binding buffer (500 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was degraded with 5 units of each RNase H (BioLabs cat #M029L) and RNase A/T1 (Thermo Scientific cat #EN0551) ...
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bioRxiv - Biophysics 2024Quote: ... PM187B20 was 5’ end labelled using 1 μL T4 polynucleotide Kinase (NEB, M0201S), 1 μL γ 32P ATP (Perkin Elmer ...
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bioRxiv - Genomics 2024Quote: ... 5 units of PolyA polymerase (E. coli or yeast as appropriate) (NEB#M0276S). The reaction was incubated at 37°C for either 7 or 30 minutes and stopped by purification with a MEGAclear column (Termofisher AM1908 ...
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bioRxiv - Cancer Biology 2024Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Bioengineering 2024Quote: ... phosphorylated at the 5’ ends with T4 polynucleotide kinase (New England Biolabs M0201), annealed to generate a double-stranded insert ...
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bioRxiv - Cancer Biology 2024Quote: ... Eluted cDNA was amplified 5-cycles (NEBNext Ultra II Q5 master mix (NEB) with Illumina TruSeq PCR primers RP-1 and RPI-X ...
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bioRxiv - Bioengineering 2024Quote: ... Tagmentation reactions were halted using 5 µL of a 50% proteinase K (NEB) solution (mixed with H2O ...
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bioRxiv - Bioengineering 2024Quote: ... Tagmentation reactions were halted using 5 µL of a 50% proteinase K (NEB) solution (mixed with H2O ...
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bioRxiv - Cancer Biology 2024Quote: ... The initial 5 cycles of PCR were performed using Q5 polymerase (NEB M0491), 200uM dNTPs (Invitrogen 10297-018 ...
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bioRxiv - Biophysics 2021Quote: ... of 0.1M sodium hydroxide: 0.02M 2-(n-morpholino) ethanesulfonic acid (MES) or 1X NEB DNase I reaction buffer (NEB B0303S ...
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bioRxiv - Molecular Biology 2020Quote: 3’ linker ligation (1x PNK buffer, 800 u T4 RNA ligase 2 truncated KQ (NEB), 80 u RNaseOUT ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 1.5 µl EndoH and 2 µl 10× GlycoBuffer 3 (all NEB) buffer at 37°C 1h ...
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bioRxiv - Developmental Biology 2020Quote: ... 3mM MgCl2) for 5 min before digesting with 0.2U/ml of DNase I (NEB). Embryos were then fixed in 4% PFA ...
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bioRxiv - Developmental Biology 2021Quote: ... To each of those tubes 5 μl of 10x CutSmart Buffer (New England Biolabs) and 2 μl of DpnI restriction enzyme (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was resuspended and treated with 7.5 units (1.5 µl) RNA 5′ pyrophosphohydrolase (NEB) for 1 h at 37°C in a total volume of 20 µl ...
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bioRxiv - Cell Biology 2022Quote: ... and then unligated linker was digested away with 25 U Yeast 5’-deadenylase (NEB) and 5 U RecJ exonuclease (Epicentre) ...
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bioRxiv - Immunology 2020Quote: ... Samples (20 μL) were loaded in DNA loading buffer (5 μL; New England Biolabs) and gels were run at 60 V for 120 mins.
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bioRxiv - Molecular Biology 2020Quote: ... RNA was treated with RppH (RNA 5’ Pyrophosphohydrolase, Art. No. M0356S, New England Biolabs) to dephosphorylate small RNAs and specifically enrich for 22G RNAs ...
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bioRxiv - Genomics 2021Quote: ... 5 µL of 5X Phusion HF reaction buffer (all from New England Biolabs, USA), 0.5 µL of 10 mM premixed dNTPs and 0.5 µL of gDNA ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 µM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: 5-10 μg RNA was diluted in 2x RNA loading dye (New England Biolabs) and size separated on 7 M Urea/15% polyacrylamide/0.5×TBE gels by gel electrophoresis ...
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bioRxiv - Biophysics 2020Quote: ... the cells were first labelled with 5 µM biotin-conjugated SNAP-tag substrate (NEB) in tissue culture medium ...
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bioRxiv - Cancer Biology 2020Quote: RNA 5’-ends were phosphorylated using 4 µl T4 PNK (NEB, catalog no. M0201) in a solution consisting of 8 µl 10x PNK buffer ...
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bioRxiv - Cell Biology 2022Quote: ... dynein-bound beads were mixed with 5 µM SNAP-Cell-TMR (New England Biolabs) for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: Escherichia coli DNA adenine methyltransferase (dam)+/dcm+ (NEB 5-alpha competent E. coli, #C2987) and dam−/dcm− (dam−/dcm− competent E ...
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bioRxiv - Immunology 2020Quote: ... about 5 μg of the genomic DNA was digested with HaeIII (New England Biolabs) and purified using phenol:chloroform extraction followed by ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 2X Blunt/TA Ligase master mix (New England Biolabs, cat#M0367S), and milliQ water up to 10 μl total volume ...
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bioRxiv - Molecular Biology 2020Quote: ... which was treated with 5 units of T7E1 (New England Biolabs, Ipswich,MA, USA) for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... the RNP injection mix [5 µM EnGen® Spy Cas9 NLS (NEB Cat# M0646T) mixed with 2.5 µM of each gRNA and Cas9 buffer] was freshly prepared ...
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bioRxiv - Zoology 2021Quote: ... we split the library and ran 5 high-fidelity Phusion (New England BioLabs, #M0530) PCRs for 12 cycles on a SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 5’ adaptor ligation using T4 RNA Ligase 1 (New England Biolabs, Ipswich, MA, M0204L). Generation of cDNA was done by using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 ng of RNA was digested by 5 U of RppH (New England Biolabs) for 2 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with 5‘-phosphate-dependent exoribonuclease XRN-1 (New England BioLabs, Inc., USA), samples were treated with RNA 5‘ polyphosphatase (Lucigen) ...
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bioRxiv - Genomics 2020Quote: ... incubated for 5 minutes and magnetically separated using a Magnetic Separation Rack (NEB #S1509S). Three 1 mL 70% ethanol washes are performed on the Eppendorf tubes ...
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bioRxiv - Biochemistry 2021Quote: ... Control samples containing only RNA were treated with RNA 5’ Pyrophosphohydrolase (RppH, NEB, M0356S) to convert 5’-PPP RNA into 5’-P RNA ...
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bioRxiv - Microbiology 2021Quote: ... dsRNA ladders and a 5’ Fluorescein-labeled RNA (300 nt) were provided by NEB and used as substrates for RNase I and RNase III activity assays ...
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bioRxiv - Cell Biology 2021Quote: ... dynein-coated beads were labeled with 5 μM SNAP-Cell-TMR (New England Biolabs) in the column for 10 min at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... coli transformations were performed using NEB 5-alpha chemically competent cells (New England BioLabs). E ...
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bioRxiv - Microbiology 2021Quote: ... 5’ RACE products were generated with Template Switching RT Enzyme Mix (New England Biolabs) using anchored poly(dT)23 and TSO (GCT AAT CAT TGC AAG CAG TGG TAT CAA CGC AGA GTA CAT rGrGrG ...
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bioRxiv - Molecular Biology 2021Quote: ... in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs). After dialysis ...
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bioRxiv - Neuroscience 2023Quote: ... containing 5 µl of collection buffer (NEBNext Single Cell Lysis Module, New England Biolabs). Noteworthy ...
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bioRxiv - Genomics 2023Quote: ... around 5 million cross-linked nuclei were digested overnight using 400 U DpnII (NEB). After digestion ...
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bioRxiv - Genomics 2023Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... 5 μl of genomic DNA was used with Luna Universal qPCR Master Mix (NEB) and primers for the R ...