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Citations for New England Biolabs :
1101 - 1150 of 9563 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and purified using a Monarch RNA Cleanup Kit (NEB, T2040S), both according to the Manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit HS DNA or RNA kit (Thermo) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated by using LunaScript RT SuperMix Kit (NEB) and qPCR was performed with the Luna universal qPCR Master Mix (NEB ...
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bioRxiv - Biophysics 2024Quote: ... purified using a PCR clean up kit (NEB, Cat# T1030L) and eluted in water ...
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bioRxiv - Biophysics 2024Quote: ... All mutations were constructed by using Gibson assembly kit (NEB). The signal peptide of murine immunoglobulin κ chain was used for expression of PDGFRβ and CSF-1R receptors ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated using the mRNA Isolation kit (NEB, S1550S). mRNA was fragmented using the NEBNext Magnesium RNA fragmentation module (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... Ribosomal RNA was removed using the rRNA Depletion kit (NEB) and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 site-directed mutagenesis kit (New England Biolabs). These substitutions did not alter protein sequences ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were extracted using Monarch Plasmid Miniprep kit (NEB) and precipitated by cold ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... with NEB Next Library Quant Kit for Illumina (NEB, E7630L) and KAPA Library Quantification Kit (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using Monarch® RNA Cleanup Kit (NEB, T2040). An RNA/DNA mix was obtained by retrotranscribing 200pmol of RNA + 200pmol of the Reserve primer (5’-TTGTCGCACGTTCGGTGTCG-3’ ...
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bioRxiv - Microbiology 2024Quote: Experiments with PURExpress in vitro protein synthesis kit (NEB, E6800) were performed as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and purified with the Monarch RNA clean-up kit (NEB). cDNA was prepared from 1 μg of RNA ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using NEBuilder HiFi DNA assembly kit (NEB). DNA was quantified on a Nanodrop Lite or Qubit ...
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bioRxiv - Neuroscience 2024Quote: ... and hybridized with NEBNext rRNA Depletion Kit v2 (NEB E7400) to substantially diminish rRNA from the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... The NebNext® UltraTM II DNA library prep kit (NEB) for Illumina was used for sequencing library preparation following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... or a region in the N-terminal part (for TGDs) were PCR amplified (see Table S4 for primer sequences) from 3D7 or IT4 gDNA (Monarch gDNA Purification Kit, NEB or QIAGEN DNA extraction kit) and cloned into pSLI (Birnbaum et al. ...
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bioRxiv - Neuroscience 2021Quote: The moPrP-eGFP_39/40 fusion construct carrying eGFP within the flexible N-terminal tail between aa 39 and 40 of mouse PrP was created using NEB® Golden Gate Assembly (New England Biolabs). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... we performed eight 100 μl PCR reactions per sample (4 μg DNA per reaction, 32 μg per mouse) using Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments ranging from 3 kb to 5 kb with 40-100 bp terminal homologies were amplified from mouse BAC RP23-51O13 with Q5 polymerase (NEB, M0491L). Approximately equal amount (100 ng ...
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bioRxiv - Systems Biology 2023Quote: ... Signals following a 1h incubation at room temperature with the appropriate HRP-conjugated secondary antibodies (anti-mouse NEB 7076S or anti-rabbit NEB 7074S) were acquired using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed three times with a PBS buffer 1X and then incubated with mouse anti-MBP (NEB, Cat# E8032L) or rabbit anti-V5 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared by amplifying the sgID-BC region from 32μg of genomic DNA per mouse using unique dual-indexed primers and the Q5 Ultra II 2x Master Mix (New England Biolabs, M0544X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragment ends were repaired using Quick Blunting™ kit (NEB), purified with QIAquick column (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were made following the NEBnext UltraII kit (NEB) and associated protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA libraries were prepared using the E7765L mRNA kit (NEB next). Paired-end read sequencing was performed using the HiSeq2500 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Assembly was done using NEB HiFI DNA assembly kit (NEB E5520) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) following the product instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... MeRIP was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... ARF1 point mutations were generated by Quick-change Mutagenesis kit (NEB) and KLD reactions ...
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bioRxiv - Cell Biology 2022Quote: ... and treated with the NEBNext rRNA depletion kit (New England Biolabs) to deplete the ribosomal RNA (rRNAs) ...
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bioRxiv - Microbiology 2020Quote: ... For 3’ adaptor ligation the NEBNext Small RNA Kit (E7560S, NEB) was used ...
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bioRxiv - Immunology 2021Quote: ... The adaptors from the NEBNext Multiplex Oligos Kits (NEB, Ipswich, MA) were ligated to the adenylated 3’ ends of the DNA fragments ...
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bioRxiv - Genomics 2020Quote: ... A quick Ligation Kit (New England Biolabs Inc., MA, CA#M200l) was used for ligating the insert and vector ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed using the (AMV cDNA kit, NEB, E6550S) according to the manufacturer’s protocol using random primers and 0.1-3 µg of total RNA per sample according to the experiment ...
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bioRxiv - Microbiology 2019Quote: ... and Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA) following the supplier protocols ...
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bioRxiv - Biochemistry 2020Quote: ... then RNAs were purified using a Monarch RNA Cleanup kit (NEB), or homemade equivalent buffers and regenerated columns,75 following the same rationale ...
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bioRxiv - Developmental Biology 2019Quote: ... Library preparation (New England Biolabs Nest Ultra RNA library prep Kit) and single read Next generation sequencing (Illumina HiSeq 2500 ...