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Citations for New England Biolabs :
1001 - 1050 of 8881 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Using the NEBNext Ultra II DNA library prep kit (NEB), the DNA was adaptor-ligated and indexed for Illumina NGS sequencing ...
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bioRxiv - Bioengineering 2023Quote: sgRNA was synthesized using the EnGen sgRNA synthesis kit (NEB). Target specific oligos (Table S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... and concentrations determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced on an Illumina NextSeq 550 ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using Ultra II RNA library kit (NEB) and samples were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Genomics 2023Quote: ... the Monarch® Genomic DNA Purification Kit (NEB, Cat# T3010) was used (10 µl Proteinase K ...
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bioRxiv - Genetics 2024Quote: ... After the library quantification via NEBNext Library Quant Kit (NEB), paired-end sequencing was performed on the tiles of each region with a sequencing depth of ∼2 million reads per tile using an Illumina NextSeq 500 instrument via a NextSeq 500/550 High Output Kit v2.
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bioRxiv - Genomics 2024Quote: ... Ultra FS II library kit (New England BioLabs, Ipswich, MA) was used ...
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bioRxiv - Neuroscience 2023Quote: ... NEBNext Ultra II RNA Library Preparation Kit (New England BioLabs) for Illumina was used ...
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bioRxiv - Bioengineering 2024Quote: ... Transcription reactions using the Hiscribe IVT kit (New England Biolabs), were assembled to a final volume of 20 μl ...
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bioRxiv - Systems Biology 2024Quote: ... using the NEBuilder Hi-Fi Assembly kit (New England Biolabs). To ensure representation of all variants in the population ...
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bioRxiv - Synthetic Biology 2024Quote: Using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) 1 µg of the amplified and linearized DNA construct was transcribed in an 80 µL reaction volume and incubated overnight at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... or Monarch PCR and DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch PCR & DNA Cleanup Kit (NEB). The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The whole coding sequence was verified by automated sequencing ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepped following NEBNext Immune Sequencing Kit’s protocol (NEB), and qPCR was used to determine cycling conditions prior to plateau ...
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bioRxiv - Plant Biology 2023Quote: ... Transcripts were purified with a Monarch RNA purification kit (NEB) and quantified with a Qubit™ RNA broad-range assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Using ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs), RNA is denatured and cDNA was synthesized ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB), and then analyzed with QuantStudio Design & Analysis Software v.1.5.1 ...
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bioRxiv - Biochemistry 2024Quote: ... using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs), or by nicking mutagenesis32,60 ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal qPCR Master Mix kit (New England Biolabs). Reactions were performed as technical replicates within each of three biological replicates ...
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bioRxiv - Microbiology 2024Quote: ... Isolation was performed using Monarch Plasmid Miniprep Kit (NEB, UK).
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bioRxiv - Developmental Biology 2024Quote: The PCR-based Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) was used to perform truncation (constructs L-P ...
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Genetics 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Bioengineering 2024Quote: ... The LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA per manufacturer protocol ...
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bioRxiv - Biophysics 2024Quote: ... using the NEBuilder HiFi DNA Assembly kit (New England Biolabs); by strictly following the protocols recommended by the manufacturers ...
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bioRxiv - Biophysics 2024Quote: ... and R88 using the Q5 site-directed mutagenesis kit (NEB). Giant spheroplasts were generated as described previously (Martinac et al. ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was purified using the MonarchR RNA Cleanup Kit (NEB). The integrity of the purified RNA was determined by running RNA in a 1% agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBuilder® HiFi DNA Assembly Kit (catalog no. E2621L, NEB) was used to clone the sequence of interest into plasmid backbone.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). We verified the sequences of plasmid DNAs purified from transformed E ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were isolated using Monarch Plasmid Miniprep kit from NEB and linearized using restriction enzyme by NEB as per manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) was used to transcribe the template DNA per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified using the Monarch® DNA gel extraction kit (NEB) and subjected to Sanger Sequencing for ORF sequence-verification prior to codon-optimization at Genscript and subcloning ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Neuroscience 2021Quote: The moPrP-eGFP_39/40 fusion construct carrying eGFP within the flexible N-terminal tail between aa 39 and 40 of mouse PrP was created using NEB® Golden Gate Assembly (New England Biolabs). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... we performed eight 100 μl PCR reactions per sample (4 μg DNA per reaction, 32 μg per mouse) using Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments ranging from 3 kb to 5 kb with 40-100 bp terminal homologies were amplified from mouse BAC RP23-51O13 with Q5 polymerase (NEB, M0491L). Approximately equal amount (100 ng ...
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bioRxiv - Systems Biology 2023Quote: ... Signals following a 1h incubation at room temperature with the appropriate HRP-conjugated secondary antibodies (anti-mouse NEB 7076S or anti-rabbit NEB 7074S) were acquired using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... The cells were washed three times with a PBS buffer 1X and then incubated with mouse anti-MBP (NEB, Cat# E8032L) or rabbit anti-V5 (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragment ends were repaired using Quick Blunting™ kit (NEB), purified with QIAquick column (Qiagen ...