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Citations for New England Biolabs :
1001 - 1050 of 3325 citations for Galectin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 µM of the 6-kb fragment was incubated with 1 unit/µL terminal deoxynucleotidyl transferase (TdT, New England Biolabs, MA, USA, #M0315S) and 0.5 mM deoxyadenosine triphosphate (dATP ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 µg of empty vector and 1 µg of purified amplicon were digested overnight with 1 µl of SphI-HF® (New England Biolabs, catalog no. R3182) in a total volume of 50 µl ...
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bioRxiv - Bioengineering 2022Quote: ... and 15ul BseYI-digested product were run side-by-side on gel (1% agarose gel in TAE buffer, 1 kb ladder (NEB, Cat. No. / ID: N3232L), most gels run at 115V for 40 minutes) ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted in 1% low melting point agarose (LMP) (Biolabs) within a 1mm diameter glass capillary ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (3 U/μl) of T4 DNA polymerase (NEB), 1 μl (10 U/μl ...
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bioRxiv - Physiology 2022Quote: ... anti-MBP (1:10,000, catalog no. E8032S, New England Biolabs); anti-CCNA2 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Excess adapter was digested by adding 1 µl RecJf (NEB) and 1 µl 5’deadenylase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-GFP (1:1000; TP401, Torrey Pine Biolabs, RRID:AB_10013661), mouse anti-GFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and 1 unit of poly(A) polymerase (New England Biolabs) and was incubated at 42°C for 1 hour followed by enzyme inactivation at 95°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L). The resulting RNA fragments were used for a reverse transcription reaction using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... 0.1mM dNTPs and 1 U/μl USER enzyme (NEB, USA). 75μl of the mix was added and incubated for 3h at 37°C ...
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bioRxiv - Genomics 2019Quote: ... 2 μl of proteinase k (800 units ml-1, NEB) were added and reacted for 1 hour at 50°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: ... and dNTPs in 1× T4 DNA ligase reaction buffer (NEB), followed by dATP-addition with Klenow ...
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bioRxiv - Microbiology 2020Quote: ... 1× Phusion HF buffer (New England Biolabs GmbH, Schwalbach, Germany) with 1.5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2019Quote: ... 2% DMSO and 1 U Phusion high fidelty polymerase (NEB) in 1x buffer provided by the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... and various amounts of the 1 kb ladder (NEB#N3232s) were run on 1% Agarose gel.
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bioRxiv - Cell Biology 2019Quote: ... 1% NP40 and PNGase-F (50 U/μl: NEB#P0704S).
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bioRxiv - Genomics 2019Quote: ... 34 µl of H2O and 1 µl of PstI (NEB) were added and restriction digestion was performed at 37°C for 4 h ...
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bioRxiv - Genomics 2021Quote: ... and 1 µl of PNK (NEB M0201, 10,000 units/ml) and incubated at 37 °C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... were ligated using 1 μl Quick T4 DNA Ligase (NEB) and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
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bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated K227Q (200U; NEB)] was added ...
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bioRxiv - Genomics 2020Quote: ... 1 µl Calf Intestinal alkaline Phosphatase (10 U; CIP, NEB) or quick CIP (10 U ...
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bioRxiv - Cell Biology 2020Quote: ... 2700 units mL−1 murine RNase inhibitor (New England Biolabs), and 0.1 M Tris-HCl (pH 8.0)).81 Primers consisted of a poly-T sequence to trap mRNA molecules ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... and 1 U/µL Murine RNAse Inhibitor (NEB, Ipswitch, MA). Labeling reactions were incubated for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM sodium vanadate (New England Biolabs, Ipswich, MA, #0758s); 50 mM sodium fluoride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Reactions were treated with Exonuclease 1 (New England Biolabs, M0293S) for 45 minutes at 37℃ ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pulse-labeled with SNAPcell-505 (1 µM; NEB) for 20 min in media ...
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bioRxiv - Genomics 2021Quote: ... 0.5 μL of 1 mg/mLbovine serum albumin solution (NEB), 1.2 μL of 50 mM NAD+ (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μL DpnI enzyme (20 U, New England Biolabs #R0176L) was added to the 25 μL PCR mixture immediately after the final extension and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: 1 U (0.5 μl) RNaseIII (NEB Short Cut RNase III), was combined with 1 μl of 10X reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 U/μL exonuclease III (New England BioLabs®), while the other half of each cleanup was not treated ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of 5 units/µl RNase H (NEB) were added and samples were incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl microccocal nuclease (New England Biolabs, Ipswich, MA, USA) and 2 μl benzonase (Merck-Millipore ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μl of T4 DNA ligase buffer (NEB, Ipswich, MA), 0.5μl of restriction enzyme (BsaI-HFv2 or Esp3I) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Lane M is DNA marker (NEB 1 Kb DNA Ladder), lane N shows the PCR product from negative control (ddH2O) ...
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bioRxiv - Developmental Biology 2020Quote: ... once with 1 mL NEBNext dA-Tailing reaction buffer (NEB) and then resuspended in 50 μL of with NEBNext dA-Tailing reaction buffer (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... 1% Triton-X 100 and 32 units Proteinase K(NEB) at 37C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: 1 ml of hydrophilic streptavidin magnetic beads (New England Biolabs) were washed 5 times with equal volume of Hybridization Buffer ...