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Citations for New England Biolabs :
851 - 900 of 3325 citations for Galectin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and murine lung slices were incubated overnight with primary antibodies in 0.1% BSA as follows: anti-CDH1 (clone EP700Y, Biozol, 1:250) or anti-CDH1-Alexa-488 (clone 24E10, New England Biolabs, 1:50), anti-Twist1 (clone Twist2C1a ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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bioRxiv - Biochemistry 2021Quote: ... the receptor was truncated at C-terminus at two positions (i.e. 1-342 and 1-351) by inserting a STOP codon by site-directed mutagenesis (NEB, Cat. no. E0554). The surface expression of WT and truncated receptor constructs were normalized to similar levels by DNA titration in HEK-293 cells ...
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bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were visualized on a 1% agarose gel using the Quick-load Purple 1-Kb DNA ladder (New England Biolabs, Ipswich, MA). Primer3 Plus (Untergasser et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in 1 mL ice-cold RNase-free cell lysis buffer supplemented with 1 μL RNase inhibitor (M0307S, NEB; Ipswich, MA). After storage in −80°C refrigerator for about 40 mins ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated with mouse anti-CaMKIIα (6G9) (1:1000, Pierce Biotechnology, Catalog #MA1-048) and rabbit anti-Gaussia luciferase (1:1000, New England BioLabs, Catalog #E8023S) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... μL supernatant were incubated with biotinylated DNA that was immobilized to streptavidin magnetic beads (1 pmol DNA to 1 μL beads; New England Biolabs, Ipswich, MA), as described in Meinhardt et al.79 ...
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bioRxiv - Plant Biology 2019Quote: ... 1 U of enzyme (New England Biolabs, Hitchin, UK) and adjusted to final volume by adding molecular biology grade pure water ...
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bioRxiv - Plant Biology 2019Quote: ... 1× of appropriate buffer (New England Biolabs, Hitchin, UK), 1 U of enzyme (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 U of T4-ligase (New England Biolabs). After ligation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and 1 μl DeepVentR exo-polymerase (New England Biolabs). The PCR thermal regime consisted of 22 rounds of 94 °C for 20 sec ...
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bioRxiv - Genomics 2020Quote: ... and 1 μl (10 U/μl) of APE1 (NEB) in a final volume of 40 μl at 37°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μl RtcB RNA ligase (New England Biolabs) to a total volume of 20 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP conjugated (New England Biolabs, E8038S, dilution 1:10000); IRDye 680RD goat anti-mouse IgG (LI-COR ...
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bioRxiv - Molecular Biology 2020Quote: ... using 8 units of T4 RNA ligase 1 (NEB) and 8 nmol of ATP in a final volume of 8 μl for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed twice in 1× NEBuffer3.1 (NEB, B7003S) and resuspended in 342 μl of 1× NEBuffer3.1 ...
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bioRxiv - Genomics 2022Quote: ... and Quick-Load 1 kb Extend DNA Ladder (NEB) to aid size determination ...
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bioRxiv - Genomics 2020Quote: ... 1 μL of 5U/μL Klenow (New England Biolabs). The beads were incubated for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 1 unit/μL of DpnI (NEB; catalogue number R0176S) was added in 5 μL of purified msDNA and digested overnight at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 1 U/μl USER enzyme (both from NEB, USA), 6% v/v lymphoprep (STEMCELL Technologies ...
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bioRxiv - Microbiology 2019Quote: ... and 1 mM of each dNTP (New England BioLabs) was aliquoted and stored at −80°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1 µL of T4 DNA ligase buffer (NEB), and underwent 50 cycles of golden gate assembly (2 minutes at 42 °C ...
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bioRxiv - Biophysics 2019Quote: ... 1% v/v Q5 hotstart polymerase (NEB, cat# M0493), 1x Q5 Reaction Buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL 1 mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 400 U/µL (NEB) ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 1 µl of BsaI 10 U/µl (NEB) were added to 7 µl of a mixture of the DNA parts used for each TU ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... anti-MBP mouse 1/80,000 (E8032S, New England Biolabs). Western Blots were quantified using Fiji ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-myc (NEB/Cell Signaling, 2276; 1:1500) o/n at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... 1 µl T4 RNA Ligase 2 truncated (200U; NEB)] was added ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.038 U μL−1 Lambda Exonuclease (NEB, M0262L)] to each well using the Mantis liquid handler and preamplified the cDNA using the following protocol ...
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bioRxiv - Bioengineering 2020Quote: ... 1 μL of Lambda Exonuclease (M0262S, New England Biolabs) and 2 μL of 10 X Lambda Exonuclease Buffer ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μL of 2 U L-1 ExoI (NEB) was added and incubated at 37 °C for 30 minutes then 80 °C for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μl/sample volume of T4 DNA ligase (NEB) was added to the master mix ...
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bioRxiv - Biophysics 2021Quote: ... re-suspended in 800 µL 1 × DpnII buffer (NEB) with 1.6% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... NEBNext Multiple Oligos for Illumina Set 1 (NEB #E7335), 2 (NEB #E7500 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of plasmid was treated with Sssl (NEB) or mock as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 1 U/μl RNase inhibitor (M0314S; New England Biolabs), and 2 mM RVC ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL of 5000 units/mL RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 10 units of T4 RNA Ligase 1 (NEB). Ligation reactions were incubated at 25 °C for 16 hours ...
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bioRxiv - Systems Biology 2021Quote: ... Inserts were purified from a 1% agarose gel (NEB), digested with NheI-HF and SphI-HF (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... and 1 mM of each dNTP (New England BioLabs)) was aliquoted and stored at −80° C ...
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bioRxiv - Systems Biology 2021Quote: ... supplemented with an additional 1 mM ATP (NEB P0756) at room temperature for 1 hour to allow ligation reaction between the 5ʹ- and 3ʹ-ends of the DNA seqFISH+ primary probes ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of Endonuclease IV (10,000 U/ml, NEB) was added to the reaction ...
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bioRxiv - Physiology 2020Quote: ... phospho-AKT (#9271, 1:1000, Cell signalling, NEB, UK), total-AKT (#9272 ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of 10x T4 DNA ligase buffer (NEB), 1 µL of T4 DNA ligase (400,000U/mL ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of T4 DNA ligase (400,000U/mL, NEB) and 2 µL ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.02 mg/ml DNase 1 (New England Biolabs, USA), 0.2 mg/ml dispase (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... 1% (vol/vol) murine RNase inhibitor (New England Biolabs), and 10% (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μL of Lambda Protein Phosphatase (New England Biolabs) or 1 μL of phosphatase inhibitor mix (20 mM β-glycerophosphate ...