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Citations for New England Biolabs :
1001 - 1050 of 2312 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... coli for protein purification were derivatives of pMal-c5X (New England Biolabs), which carries an AmpR marker and uses an IPTG-inducible tac promoter to expresses recombinant proteins with a factor Xa cleavable maltose-binding protein (MBP ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were expressed in Escherichia coli T7 Express (New England Biolabs) and grown in Luria-Bertani medium ...
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bioRxiv - Microbiology 2020Quote: ... protein extracts were treated with PNGase F according to manufactures instructions (NEB).
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bioRxiv - Immunology 2020Quote: The fusion protein was treated with glycerol free PNGase F (NEB, P0705S) in non-denaturing reaction conditions ...
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bioRxiv - Bioengineering 2021Quote: ... the eluted MBP-RFP-sspB proteins were cut with TEV protease (NEB) using a digestion site between the MBP and the RPF sections ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein digestion was achieved by sequential addition of endopeptidase Lys-C (BioLabs) and trypsin (Pierce™ ...
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bioRxiv - Developmental Biology 2022Quote: ... The bait protein vector was digested with AscI/BamHI (New England Biolabs) to remove the tag encoding region and then ligated to an eGFP insert generated by PCR using primers with AscI and BamHI tails (see Table S5 for primer sequences) ...
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bioRxiv - Immunology 2020Quote: ... 1x NEB buffer for Protein Metallophosphatase and 1mM MnCl2 (New England Biolabs) for 30 min at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... samples were diluted with 3x Blue Protein Loading Dye (New England Biolabs) and boiled for 5 minutes ...
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bioRxiv - Physiology 2021Quote: ... Proteins were then deglycosylated with PNGaseF (New England Biolabs, Ipswich, MA, USA) and digested with Lys-C (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... the protein was batch bound to 10 mL of chitin resin (NEB) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GFP-fusion proteins were purified from BL21(DE3) cells (NEB, # C2527H) using an N-terminal H6-tag ...
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bioRxiv - Biochemistry 2020Quote: Proteins have been expressed in Escherichia coli strain BL21 (DE3) (NEB # C2527). In brief ...
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bioRxiv - Immunology 2021Quote: ... The lysate and the precipitates were treated with Lambda Protein Phosphatase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 μg protein was mixed with 15 μL Glycoprotein Denaturing Buffer (NEB) in 150 μL total volume and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... while the other was treated overnight with Protein Deglycosylation Mix II (NEB) using non-denaturing reaction conditions according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... the MBP-fusion proteins were purified using amylose resin (New England Biolabs) and GST-fusions by glutathione-resin (GE Healthcare) ...
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bioRxiv - Plant Biology 2022Quote: ... incubated with λ protein phosphatase (40 units/μL) (P0753S, New England Biolabs) or recombinant TOPP4-GST/HIS for 1 h at 30□ ...
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bioRxiv - Plant Biology 2022Quote: ... MBP-fusion proteins were purified using Amylose Resin (New England Biolabs, E8021), following the manufacturer’s instruction with minor modification ...
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bioRxiv - Microbiology 2023Quote: ... and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs) using the 4D-Nucleofector system (Lonza) ...
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bioRxiv - Molecular Biology 2024Quote: ... including a Blue Pre-stained Broad Range Protein Standard (New England Biolabs). Gels were either stained (with 0.5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified on glutathione-Sepharose beads (New England Biolabs), and MBP-tagged proteins were purified on Amylose resin (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used as described previously (31) ...
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bioRxiv - Cell Biology 2023Quote: ... 25 µl protein A magnetic beads S1425S (New England Biolabs; Ipswich, MA) were washed 3 times in PBS and incubated overnight with 2-3 µg of antibody directed against CISD34 at 4°C with gentle mix ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNA was incubated at room temperature with Cas9 protein (NEB, Cat# M0646T). AB* embryos were injected at the one-cell stage with the sgRNA and Cas9 cocktail in a 1nL volume at 25pg sgRNA/nL ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were lysed and subsequently incubated with λ-protein phosphatase (NEB) for 1 h at 30°C according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were purified by NEBExpress Ni Spin Columns (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins with MBP-tag were loaded onto amylose resin (New England Biolabs) after elution from Ni-NTA ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL of 2X protein synthesis buffer (New England Biolabs, Beverly, MA), 0.25 μL of RNaseOUT RNAse inhibitor (Thermo-Fisher ...
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bioRxiv - Genetics 2024Quote: ... and 400 units of Lambda protein phosphatase (λpp) (New England Biolabs, P0753S). The reactions were incubated at 30°C for 30 minutes and terminated by an equal volume of 2x Laemmli buffer.
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bioRxiv - Microbiology 2024Quote: ... MBP fusion proteins were detected with a rabbit anti-MBP from NEB company 1:1000 followed by an incubation with a goat anti-rabbit HRP-conjugated (1:3000) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Microbiology 2024Quote: ... the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, E6800L) was used according to the manufacturer’s instructions ...
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Higher meiotic chromosome condensation: a potential function of kinetochore through polo-like kinasebioRxiv - Cell Biology 2024Quote: ... The diluted sample was treated with 2 µl Lambda protein phosphatase (NEB) and incubated in water bath at 30 ℃ for 4 hrs ...
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bioRxiv - Biochemistry 2024Quote: ... 2.5 μL 10 mM MnCl2 and 2 μL Lambda Protein Phosphatase (NEB). The dephosphorylation reaction was allowed to occur at 30 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... and New England Biolabs PURExpress In Vitro Protein Synthesis Kit (NEB #E6800). All E ...
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bioRxiv - Biochemistry 2024Quote: ... with or without 2 µL Lambda protein phosphatase (NEB, 400000 U/mL). Reactions were carried out for various times ...
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bioRxiv - Cancer Biology 2024Quote: ... All proteins were expressed in NEB 10-beta cells (New England Biolabs). Cells transformed with the corresponding expression vector were grown in LB medium supplemented with 100 µg/ml Ampicillin at 37 °C to an OD600 of 0.8 ...
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bioRxiv - Cell Biology 2024Quote: The protein expression plasmid was transformed into BL21-DE3 cells (NEB # C2527H). The cells were streaked onto LB agar plates containing the corresponding antibiotic ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs), or mouse monoclonal anti-β-actin antibody (A5316 ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-butyryllysine antibody (PTM BioLabs, PTM#301) at 1:2000 dilution was incubated with the membrane overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... a barcoded RNA adapter (RiL 19) was ligated to the 3’ end using T4 RNA ligase (NEB). The 3’ adaptor ligated RNA was again cleaned up as mentioned above ...
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bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
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bioRxiv - Microbiology 2021Quote: ... After dephosphorylation of the 3′ ends of the recovered RNA using T4 polynucleotide kinase (T4 PNK; NEB), RNA was ligated to Linker-1 (5′-App CTGTAGGCACCATCAAT ddC-d′ ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations on smaller fragments of the RBP10 3′-UTR were done using either site directed mutagenesis (NEB, Q5® Site-Directed Mutagenesis Kit Quick Protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations on smaller fragments of the RBP10 3’-UTR were done using either site directed mutagenesis (NEB, Q5® Site-Directed Mutagenesis Kit Quick Protocol ...