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Citations for New England Biolabs :
851 - 900 of 2312 citations for Fc Receptor Like Protein 3 FCRL3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... SNAP-tagged proteins were labelled with Cell-SiR647 (NEB, Frankfurt, Germany). Labeling was done at RT for 1hr ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein-containing fractions were pooled and incubated with Chitin Resin (NEB) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µL of Colour Prestained Protein Standard (New England Biolabs #P7719S) was included on each gel ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were mixed with Cas9 protein (M0646M NEB, or B25641 invitrogen) and coinjected into one-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Cell Biology 2022Quote: Protein extract samples (5µl) were subjected to EndoHf (New England Biolabs) treatment according to manufactures instructions ...
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bioRxiv - Genomics 2022Quote: ... EnGen Spy Cas9 NLS protein (20 μM) was purchased from NEB. The injection mixture consisted of 600 ng/μl Cas9 protein ...
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bioRxiv - Genetics 2022Quote: ... 0.8pg of 3xNLS-SpCas9 protein and 1mU of I-SceI (NEB) was injected per embryo at early 1-cell stage ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... We washed 80µL Protein A/G Magnetic Beads (New England Biolabs) per sample with bead washing buffer (PBS with 0.1% BSA and 2mM EDTA ...
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bioRxiv - Microbiology 2024Quote: The PURExpress ΔRibosome In Vitro Protein Synthesis Kit (New England Biolabs) was used for in vitro translation assays ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A mixture of 600 ng/µL of Cas9 protein (NEB: M0386) and sgRNA complex was prepared at a 1:1 ratio and 1 nL of the mixture was injected into zebrafish embryos at the one-cell stage ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified heterotrimer was de-phosphorylated by lambda protein phosphatase (NEB), calf intestinal phosphatase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... motor protein ligation (NEBNext Quick Ligation Module, Cat. No. E6056, NEB) and the addition of loading beads (Ligation Sequencing Kit ...
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bioRxiv - Microbiology 2024Quote: ... using the Broad Range Blue Prestained Protein Standard (Νew England Biolabs). Electrophoresis was carried out at 100 V for 85 min with a Bio-Rad Mini-Protean3 electrophoresis system.
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bioRxiv - Biochemistry 2024Quote: ... The protein was digested by adding 2 µL of PK (NEB) to the mixture and incubating at 45°C for 45 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 25μL of the samples and 1μL of the protein ladder (BioLabs) were loaded into a Bio-Red 4-15% Mini-PROTEAN precast polyacrylamide gel ...
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bioRxiv - Plant Biology 2024Quote: ... MBP-fusion proteins were enriched via amylose resin (NEB, Ipswich, USA), while GST-fusion proteins were enriched PierceTM glutathione agarose resin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... protein-bound RNAs were dephosphorylated on bead using T4 PNK (NEB) with modified PNK Buffer (10mM Tris pH 6.5 ...
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bioRxiv - Genetics 2024Quote: ... The protein was then bound to amylose resin (New England Biolabs) via the MBP tag on the MMS22L subunit ...
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bioRxiv - Synthetic Biology 2024Quote: NEB PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800S) supplemented with RNase Inhibitor (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was sequentially treated with lambda protein phosphatase (PPase) (NEB) and benzonase (EMD Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo-) (NEB M0212S) and ligation with Illumina sequencing adapters using T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Top and bottom guide RNAs were annealed with T4 Polynucleotide Kinase (3’ phosphatase minus, NEB) and T4 DNA Ligase Buffer (NEB ...
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bioRxiv - Genomics 2022Quote: ... Genomic DNA was dephosphorylated by adding 3 μl of Quick CIP enzyme (NEB, Cat #M0508) to 5 μg of input DNA (in total volume of 24 μl at >210 ng/μl ...
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bioRxiv - Genomics 2020Quote: ... add 2 μl 10 mM dATP and 5 μl Klenow Fragment (3’ −> 5’ exo-) (NEB) to digest the unligated DNA fragments at 37°C for 40 min ...
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bioRxiv - Genomics 2021Quote: ... rRNA was removed from 3 replicates with NEBNext Bacteria rRNA Depletion Kit (New England Biolabs), while the other 3 were treated with NEBNext Depletion Core Reagent Set using custom probes targeted to Haloferax volcanii rRNA (Martinez-Pastor and Sakrikar ...
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bioRxiv - Genomics 2021Quote: ... The ligation mix (5 μL of LNB, 3 μL of Quick ligase (New England Biolabs), 1.2 μL of MQ ...
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bioRxiv - Pathology 2021Quote: ... The second strand was synthesized with Klenow Fragments (3′-5′ exo-; New England Biolabs Inc.) and complement chains of NSR primers ...
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bioRxiv - Neuroscience 2020Quote: ... These 3 DNA fragments were assembled into a vector backbone (NEB pMiniT vector, EcoRI linearized) with the NEBuilder HiFi DNA Assembly Cloning Kit (NEB Cat# E5520) ...
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bioRxiv - Biochemistry 2020Quote: ... was replaced with sequence for a 3×FLAG tag by Gibson Assembly (New England Biolabs) to create payload plasmid pSAB41 ...
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bioRxiv - Microbiology 2021Quote: ... coli MG1655 genomic DNA into pKK223-3 by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) and the first 300 bp portion of leuA was translationally fused with the nano-Luc (nLuc ...
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bioRxiv - Genomics 2022Quote: ... dA was added to the 3’-end of DNA using Klenow fragment exo- (NEB: M0212S) and then the adapter was ligated to the end of DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’ ligation (on-bead) with a barcoded RNA adapter followed using T4 RNA Ligase (NEB). Samples were again stringently washed ...
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bioRxiv - Developmental Biology 2022Quote: ... and the 3’ homology arm were produced by PCR (Phusion DNA polymerase, New England Biolabs). The 5’ and 3’ homology arm DNA fragments were amplified from genomic DNA prepared from vasa-Cas9 flies ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.1 mM dATP and 15 units of Klenow Fragment (3’→5’ exo-) (NEB, M0212M) and incubated at 37°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... CrPV 5’UTR-1A-GFP-3’ UTR was generated using Gibson assembly (NEB Gibson assembly). The respective mutants were generated using Site directed mutagenesis ...
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bioRxiv - Molecular Biology 2020Quote: ... 240 μM TruSeq Universal Adapter (5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’) and 0.025 U Taq DNA Polymerase (NEB) in 1X Standard Taq Reaction Buffer (NEB) ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2021Quote: ... to 3’-P presenting in the RNA that was dephosphorylated using T4 PNK (NEB, #M0201S). Linker and RNA ligation was performed by ligating pre-adenylated linker to the 3’-OH of RNA using RNA ligase2 ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Molecular Biology 2020Quote: 3’ linker ligation (1x PNK buffer, 800 u T4 RNA ligase 2 truncated KQ (NEB), 80 u RNaseOUT ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2.0 units of Klenow Fragment (3’-5’ exo−)(New England Biolabs, Ipswich, Massachusetts, USA), total 10 μl of the mixture was incubate at 37°C for 90 min ...
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bioRxiv - Genomics 2023Quote: ... followed by 23.5 μl of ligation mix (3 μl 1x T4 ligase buffer (NEB, B0202S), 0.15 μl 50 mg/ml BSA (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were incubated with 1.5 µl EndoH and 2 µl 10× GlycoBuffer 3 (all NEB) buffer at 37°C 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were 3’ dephosphorylated and 5’ phosphorylated with T4 polynucleotide kinase (T4PNK, NEB, #M0201S), and purified with RNA Clean and Concentrator-5 (Zymo Research ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and second strand cDNA synthesis (using Klenow fragment 3’-5’ exo- [New England BioLabs, USA]) of chicken fecal samples and dust samples was performed as previously described 55 and following manufacturer’ instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 3 μg purified DARPP-32 isoforms as well as 5 μl ATP (New England Biolabs) in kinase dilution buffer III (SignalChem ...
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bioRxiv - Cell Biology 2022Quote: ... A18-Y260A-SDM-Rev (5’-CGTTGTGTAACCAGGAGAATG-3’) and Q5 site directed mutagenesis kit (NEB E0554). This LT3N1-GPIR-Arhgef18 vectors were further modified to substitute EGFP with 3XHA-FLAG tag ...
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bioRxiv - Cell Biology 2022Quote: ... miRE-Rv (5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’) and Q5 Hot Start High-Fidelity DNA polymerase (NEB M049S). The final amplicon was then digested and cloned into LT3GEPIR in-between XhoI and EcoRI restriction sites as previously described2 ...