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Citations for New England Biolabs :
951 - 1000 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The eluted DNA fragments were purified on a Monarch PCR & DNA Cleanup Kit (#T1030S, New England BioLabs and eluted with 26 μL DNAase and RNAase free water ...
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bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Bioengineering 2021Quote: ... were PCR-amplified and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
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bioRxiv - Bioengineering 2021Quote: ... the PCR reaction was purified via Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). Plasmids were constructed via the Hot Fusion Assembly Method (Fu et al. ...
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bioRxiv - Microbiology 2020Quote: ... aeruginosa PA14 genomic DNA (gDNA) and cleaned up using the Monarch PCR Purification kit (New England Biolabs) were used for Gibson assembly together with pMQ30 cut with SacI and HindIII ...
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bioRxiv - Microbiology 2019Quote: ... The DNA was isolated from the supernatant using the PCR clean-up kit (New England Biolabs®) and quantified using a nanodrop ...
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bioRxiv - Microbiology 2022Quote: ... and mucI PCR products were ligated into pBGE using the Gibson Assembly Cloning Kit (New England Biolabs) to generate pmucG-C ...
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bioRxiv - Neuroscience 2020Quote: ... DNA from 2 biological replicates was purified with Monarch PCR & DNA Cleanup Kit (Cat. no. T1030, NEB). Library preparation ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were ligated using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA) and transformed into competent DH5a E ...
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bioRxiv - Synthetic Biology 2022Quote: ... The two PCR fragments were then assembled using the HiFi Gibson Assembly kit from NEB (Waltham, MA) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2019Quote: ... the DNA from the PCR reaction was purified using a clean-up kit (T1030, New England Biolabs) and sequenced using SimpleSeq™ tubes (Eurofins Genomics ...
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bioRxiv - Immunology 2021Quote: ... The reaction was purified using a PCR clean-up kit according to manufacturer’s directions (NEB, Cat#: T1030S) or using ExoSAP-IT ...
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bioRxiv - Developmental Biology 2019Quote: ... Guide RNAs were generated by PCR and transcribed using HiScribe T7 High-Yield RNA Synthesis Kit (NEB). 110-140 pg of guide RNA and 170 pg of cas9 mRNA per embryo was injected at one-cell stage into the cell ...
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bioRxiv - Microbiology 2020Quote: ... Specific gRNA and KOD PCR were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (42 ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR fragment and the plasmid (pOPINS3C) were assembled using the NEBuilder HiFi DNA Assembly kit (NEB), following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were amplified by PCR and assembled by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S).
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bioRxiv - Synthetic Biology 2022Quote: ... The two PCR fragments were then assembled using the HiFi Gibson Assembly kit from NEB (Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products corresponding to the correct size were excised and purified using DNA Gel Extraction kit (NEB). 100 ng of each gel-purified PCR products (total of 19 ...
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bioRxiv - Biochemistry 2022Quote: The amplicons described above were purified with the Monarch PCR and DNA purification kit (New England Biolabs) and sent for NGS EZ Amplicon Sequencing by Genewiz ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-free libraries were prepared with the NEBNext UltraTM II DNA Library Prep Kits (New England BioLabs) according to supplier recommendations ...
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bioRxiv - Microbiology 2023Quote: ... rLCMV-GP1-FLAG was generated by insertion PCR utilising Q5 site-directed mutagenesis kit (New England BioLabs) and FLAG primers ...
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bioRxiv - Microbiology 2023Quote: ... and RpoD control were assessed using the Luna Universal One-Step qRT-PCR kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
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bioRxiv - Cell Biology 2024Quote: ... The synthesized probes were then purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs) according to the manufacturer’s instructions and the quality was verified on denaturing 12-16% polyacrylamide gels.
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bioRxiv - Genomics 2024Quote: ... and used for the cloning of PCR fragments with a Gibson Assembly cloning kit (New England Biolabs) following the manufacturer’s instructions ...
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Linker-ligated RPFs were reverse transcribed with ProtoScript II RT (NEB) and gel-purified from 15% TBE-Urea gels ...
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bioRxiv - Cell Biology 2020Quote: ... After ligation at RT for 4 h with T4 ligase (NEB), the nuclei were pelleted ...
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bioRxiv - Immunology 2022Quote: ... 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Molecular Biology 2019Quote: ... with Luna Universal One-Step RT-qPCR reagents (New England Biolabs). 50 ng total RNA and 250 nM primers were added in each reaction ...
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bioRxiv - Synthetic Biology 2021Quote: ... RT-qPCR (Luna Universal qPCR Master Mix; New England BioLabs; M3003L) was performed in 384 well plates (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 4 μL LunaScript RT SuperMix 5X (New England Biolabs, NEB, USA) and 8 μL nuclease free water ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μl ProtoScript II RT (200 U/μl, New England Biolabs), and 2 μl 100μM template-switching oligonucleotide TSO (Supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription (RT) was performed with M-MuLV reverse transcriptase (NEB) according to manufacturer instructions with a short oligonucleotide to prevent annealing of the oligonucleotides on RNAs that lost a few nucleotides at the 3’ end during the migration (and would thus run faster in the reverse run ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was performed using the LunaScript RT SuperMix (NEB E3010) and 1μg DNase-treated total RNA with incubation at 25ºC for 2 min ...
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bioRxiv - Genomics 2023Quote: ... Viral RNA was reverse transcribed using LunaScript RT SuperMix (NEB #E3010) to generate cDNA as described (Freed et al ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: ... cDNA was generated using the Induro RT reverse transcriptase system (NEB). PCRs were conducted using Q5 DNA polymerase (NEB ...
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bioRxiv - Genomics 2023Quote: Each library was converted to cDNA with Induro RT (NEB M0681S) using a 57-mer ssDNA as a primer ...
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bioRxiv - Microbiology 2024Quote: ... and reverse transcription was performed with ProtoScript II RT enzyme (NEB) using barcode primers (Supplementary Table S1) ...
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bioRxiv - Bioengineering 2024Quote: ... mRNA was then reverse-transcribed using LunaScript RT-supermix (NEB #E3010). Genotyping PCR was then conducted using Q5 polymerase (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... The time between nuclear envelope breakdown (NEB) and anaphase onset (AO ...
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bioRxiv - Cell Biology 2023Quote: PCR with the teltail and P1 PCR primers was performed using Q5 PCR enzyme mix (NEB, #M0494) and the following conditions using 0.1 μl DNA-bound beads ...
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bioRxiv - Molecular Biology 2021Quote: PCR was performed with Taq polymerase PCR (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Genetics 2019Quote: ... Each PCR (20 μl) contained 1× standard PCR buffer (NEB), 1 U of Taq polymerase (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... before PCR using NEB 2xphusion PCR mastermix (NEB, cat #M0531S).
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bioRxiv - Evolutionary Biology 2021Quote: ... Products from the first round PCR were then purified using Monarch DNA Gel Extraction Kit (New England Biolabs) and used as the templates for the second round insert PCR ...
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bioRxiv - Genomics 2019Quote: ... The amplicons were size-selected (∼600 bp) and cloned using the NEB PCR Cloning Kit (New England Biolabs). The resulting amplicons were Sanger-sequenced at Eurofins Genomics (Cologne ...
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bioRxiv - Cell Biology 2021Quote: ... and PCR amplification were performed using the Truseq Small RNA Sample Prep Kit for Illumina (NewEngland Biolabs, E7335S) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... were amplified by PCR and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
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bioRxiv - Biochemistry 2020Quote: ... The DNA was immediately purified using a DNA cleanup column (the Monarch PCR & DNA cleanup kit from NEB) and eluted with water ...