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Citations for New England Biolabs :
951 - 1000 of 1635 citations for Recombinant Human ROR1 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The unbound fraction was dephosphorylated using lambda protein phosphatase (NEB), calf intestinal phosphatase (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... kit and co-injected with Cas9 protein (New England Biolabs) into one-cell stage zebrafish eggs ...
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bioRxiv - Cell Biology 2023Quote: ... in the presence of protein kinase buffer (New England Biolabs) with 3 μCi [γ-32P]ATP ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µl of prestained protein ladder (New England Biolabs). The gel was electrophoresed at 150 V ...
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bioRxiv - Cell Biology 2024Quote: ... STAT1 proteins were dephosphorylated by lambda phosphatase (NEB, Ipswich MA) followed by the addition of protease and phosphatase inhibitors (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL of protein G beads (New England Biolabs (NEB), S1430S ...
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bioRxiv - Neuroscience 2021Quote: ... Human α-synuclein cDNA from a pcDNA3.1 plasmid was restriction digested with EcoRI and HindIII (NEB), purified and ligated in the same sites of the pAAV2.5-THP backbone to generate the pAAV2.5-TH-α-syn plasmid ...
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bioRxiv - Biophysics 2022Quote: ... The human μOR and V2R were amplified with a SNAP tag at their N-terminal (NEB) and subcloned in the pcDNA4/TO plasmid (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) according to the manufacturer’s instructions with 6 µl total RNA used as input per sample ...
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bioRxiv - Neuroscience 2022Quote: ... made from pCX with the promoter replaced by human Synapsin (hSyn) using golden gate assembly (NEB). For synaptic labelling pCAG-GPHN-FingR-EGFP-CCR5TC40 Addgene # 46296 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were prepared using the NEBNext rRNA Depletion Kit v2 (Human/ mouse) (NEB #E7405), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Genetics 2019Quote: ... Exon 14b was amplified with primers F: GACATGTTGCTAAGATTGAAATCCGT from exon 14 and R: GACCCAGCTTTCAGAGTAACCAGAAC from exon 15 using Phusion polymerase (NEB, Ipswich, MA). The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch ...
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bioRxiv - Developmental Biology 2021Quote: ... touchdown PCR (with primers atg13 F- GGCTCGTGCGACAATGGATAGTG; R- GACCTCGGGGATGTCCTTTATTGC) was followed by a HindIII restriction digest (R3104S, New England Biolabs, MA, USA), and fragments were separated by gel electrophoresis on a 3% agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were propagated in Escherichia coli NEB Turbo ((F’ proA+B+ lacIq ΔlacZM15/fhuA2 Δ(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10) TetS endA1 thi-1 Δ(hsdS-mcrB)5)) (New England Biolabs) at 37°C in lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2022Quote: ... We then carried out rRNA depletion with the NEBNext rRNA Depletion Kit (Human/Mouse/Rat NEB # E6310S) using the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... SUMO1 and SUMO3 were amplified from human cDNA and cloned in the pTXB1 vector (New England Biolabs) by restriction cloning according to the manufacturers protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Human RAC1P29S cDNA was cloned into the vector using EcoRI and XhoI restriction enzymes (New England Biolabs) and ligated using T4 ligase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Biochemistry 2024Quote: The cloning of human wild type Mad2 and the mutant Mad2R133A by the USER® method (NEB) into the pRSFDuet-1 vector (71341-3 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein dephosphorylation was performed on total WT and MLP knockout heart lysates by using Alkaline Phosphatase (rSAP)(M0371S NEB, 1U of rSAP/10mg of protein). The protein lysate containing phosphatases ...
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bioRxiv - Microbiology 2020Quote: ... The soluble proteins were mixed with amylose resin (New England Biolabs) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2020Quote: The protein was expressed in NiCo21 (DE3) cells (New England Biolabs) grown in Overnight Express Instant TB Medium (EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-Maltose Binding Protein monoclonal antibody IgG2a (New England Biolabs), mouse anti-Histone H3 [Trimethyl Lys9] 6F12-H4 (Novus Biologicals) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with DTT and proteins were degraded using proteinase K (NEB). Total cellular RNA was purified using the RNeasy maxi kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (New England Biolabs, #M0646M, EnGen Cas9 NLS 20 μM) and 300 pg of sgRNAs along with 5 ng of GFP cRNA were co-injected into the X ...
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bioRxiv - Developmental Biology 2021Quote: ... in equimolar ratios and EnGen®Spy Cas9 NLS protein (NEB) into 1-cell zebrafish embryos.
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bioRxiv - Immunology 2021Quote: ... Deglycosylation was performed on beads using Protein Deglycosylation Mix II (NEB) in denaturing conditions according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... associated with EnGen™ Spy Cas9 NLS protein (New England BioLabs), using the 4D-Nucleofector™ System (Lonza ...
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bioRxiv - Physiology 2020Quote: ... were co-injected with 0.5 μl Cas9 protein (NEB, M0386, 20μM), diluted 1:10 ...
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bioRxiv - Cell Biology 2019Quote: ... MBP-MKLP1 proteins were purified on amylose resin (New England Biolabs), washed 5 times with Washing Buffer (50 mM Tris-HCl pH 7.5 ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... MBP-fusion proteins were purified using Amylose resin (New England Biolabs), washed in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Molecular weights were identified using a protein standard (BioLabs, Cat# P7712S). Beta-actin was used as the loading control ...
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bioRxiv - Biochemistry 2020Quote: ... morsitans salivary proteins were treated with PNGase F (New England Biolabs), resolved by SDS-PAGE and transferred onto a PVDF membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µl of lambda protein phosphatase (New England BioLabs, Ipswich, MA). Each mixture was incubated at 30 °C for either 1 ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were then coupled to protein G beads (New England Biolabs). After beads were washed ...
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bioRxiv - Plant Biology 2020Quote: ... the purified proteins (0.125 mg) or RNase If (1.25 U; NEB) were incubated at 25°C for 60 minutes with a fluorescent-labeled RNA substrate (5’-fluorescein isothiocyanate (FITC)-CUUUUAG(A20) ...
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7719).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then digested with LysC endoproteinase (0.25 µg; NEB) for 4 hours at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... MBP fusion proteins were purified on amylose beads (New England Biolabs) in column buffer (20mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was first bound to amylose resin (New England Biolabs). Afterwards ...
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bioRxiv - Cancer Biology 2019Quote: ... After cooling 5 µl Protein Deglycosylation Mix II (New England Biolabs) was mixed in gently ...
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bioRxiv - Bioengineering 2020Quote: IVPS was performed using PURExpress In Vitro Protein Synthesis Kit (NEB) with supplements (16 U RNase inhibitor (NEB) ...
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bioRxiv - Pathology 2021Quote: ... Soluble protein extracts were purified using amylose resin (New England Biolabs) and/or Ni-NTA agarose beads (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Magnetic beads conjugated with IgG Protein A/G (New England Biolabs) were pre-blocked with 0.5 mg/mL of sonicated salmon sperm DNA (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 100μg/ml single stranded DNA binding protein (gp32) from T4 (NEB), 277μg/ml T4 DNA clamp ...
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bioRxiv - Biochemistry 2021Quote: ... the purified proteins were incubated with excess streptavidin (New England Biolabs) for 10 minutes before analysis via gel-shift using SDS-PAGE ...