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Citations for New England Biolabs :
51 - 100 of 2482 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The end repaired dsDNA was amplified for 15 cycles using 10 μL of NEBNext 2X High Fidelity PCR MM (NEB), and 0.2 μL each of primers Solexa 1GA and Solexa 1GB (stock concentration of 100 μM and final concentration of 1 μM) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μL 10× Poly(A) Polymerase Reaction Buffer and 1 μL Poly(A) Polymerase (NEB) for poly(A ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified by 13 cycles of PCR with Phusion polymerase (New England Biolabs). A size selection step was done using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... 17 μl of the lysates was treated with 500 U PNGase F (NEB, cat# P0704S), 500 U Endo H (NEB ...
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bioRxiv - Genomics 2024Quote: ... resuspended in 17 µl water and proceeded with nucleoside digestion reaction (NEB, catalog number: M0649) to prepare for LC-MS.
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bioRxiv - Developmental Biology 2023Quote: ... and 3.3 kb DNA sequence upstream of each respective gene was amplified using PCR and cloned into the vector containing the sequence gfp::rab-7::rab-7 3’UTR amplified from plasmid rgef-1p::gfp::rab-7::rab-7 3’UTR using PCR and primers 5’-atgagtaaaggagaagaacttttca-3’ and 3’-aagcttatcgataccgtcgac-5’ were used to generate tissue-specific gfp::rab-7::rab-7 3’UTR by using Gibson assembly (NEB E2611).
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bioRxiv - Developmental Biology 2023Quote: ... rgef-1p and gfp were inserted into a vector containing the sequence rab-7 amplified using PCR and primers 5’-atgtcgggaaccagaaagaa-3’ and 3’-aagcttatcgataccgtcgac-5’ to create rgef-1p::gfp::rab-7::rab-7 3’UTR using Gibson assembly (NEB E2611). A full list of reagents and resources can be found in table S2.
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bioRxiv - Molecular Biology 2023Quote: ... 3-4 μg of sperm gDNA was dephosphorylated with 10 μl of rSAP (NEB, no. M0371S) and 16 μl of 10× rCutSmart buffer (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
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bioRxiv - Neuroscience 2021Quote: The plasmid trans-Tango MkII was generated using HiFi DNA Assembly (New England Biolabs #2621) and was incorporated into the attP40 locus using the ΦC31 system as described in the original trans-Tango paper4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 15□l of 2.1 buffer (NEB), 30 units of SSP1 (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... 16.4 mL elution was taken into 40 mL ligation reaction (13 Quick ligase buffer [NEB] ...
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bioRxiv - Microbiology 2021Quote: ... Pooled samples were clarified by centrifugation (10 000 g, 15 min) and treated with DNase I and RNase If (NEB, Great Britain) for removing of naked out-capsid nucleic acid ...
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bioRxiv - Genetics 2023Quote: ... 1 ul of 5 mM 7-deaza-dGTP (NEB), 1 ul of 25 mM MgCl2 (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... 7 μL Murine RNase Inhibitor (New England Biolabs, M0314) was added ...
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bioRxiv - Microbiology 2020Quote: ... 14-17 A260 of lysate was digested with 800 U/A260 of micrococcal nuclease (MNase, NEB) at 25°C with shaking at 14,500 rpm for 20 min in lysis buffer supplemented with 2 mM CaCl2 and 500 U RNase Inhibitor ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
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bioRxiv - Neuroscience 2023Quote: Plasmids for the trans-Tango components were generated using the Hi-Fi DNA Assembly (NEB #E5520S), BP Gateway Cloning (Thermo Fisher ...
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bioRxiv - Genetics 2022Quote: ... or 13 cycles using oligos CT279 and CT297 with Phusion polymerase and HF reaction buffer (NEB). Each reaction was then analyzed by PAGE on a 12% TBE gel ...
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bioRxiv - Neuroscience 2022Quote: DNA was purified using the Monarch PCR and DNA cleanup kit (New England Biolabs, 13-0041) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 17 PCR cycles were performed using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB, M0532) with primers and template oligo each at a concentration of 10uM ...
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bioRxiv - Microbiology 2022Quote: ... the above construct was amplified using primers 17 and 18 and integrated into BamHI/PacI-digested (NEB) pUPRT plasmid by Gibson assembly ...
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bioRxiv - Biochemistry 2023Quote: ... 17 μl of the lysates was treated with 1 μl of Deglycosylation mix II (NEB, cat# P6044S) or distilled water ...
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bioRxiv - Genomics 2022Quote: ... and 15 units of Quick CIP (NEB) by incubating at 37°C for 20 min followed by Quick CIP inactivation at 80°C for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... 15 units of Exonuclease V (RecBCD; NEB) and T5 exonuclease (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... 15 units of T4 DNA polymerase (NEB) and 5 units of Klenow DNA polymerase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a 15 min DNaseI (NEB) digestion at 37°C ...
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bioRxiv - Genetics 2022Quote: ... followed by 15 min DNAse I (NEB) treatment at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... 15 μL T4 ligase (NEB, cat#M0202S), 30 μL T4 ligase buffer and ...
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bioRxiv - Cell Biology 2024Quote: ... amplified by PCR (15 cycles, Phusion, NEB) and cloned into the XhoI and EcoRI sites of pSC2 as previously described(38 ...
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bioRxiv - Molecular Biology 2023Quote: ... 7 µL Ultra II End-prep reaction buffer (NEB, E7647A), 3 µL Ultra II End-prep enzyme mix (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... Cell extracts (average OD 7) were treated with MNase (NEB), CaCl2 (5mM final concentration) ...
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bioRxiv - Molecular Biology 2024Quote: ... 7 µL Ultra II End-prep reaction buffer (NEB, E7647A), 3 µL Ultra II End-prep enzyme mix (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... for 12 h at 4 °C with 10 μg of Factor Xa protease (New England Biolabs, Hitchin, UK) per 1 g of mitochondria ...
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bioRxiv - Biophysics 2024Quote: ... A 4°C AKTA Pure FPLC system (Cytiva) was prepared with a 10 mL amylose column (NEB #E8021S) and 2 mL/min flow rate ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified DNA was eluted in 17 μL H2O and treated with exonuclease (New England Biolabs, ordering info missing). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 units USER Enzyme for 30 minutes at 37°C (NEB)51 ...
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bioRxiv - Genomics 2021Quote: ... 7 μL 20 μg/μL proteinase K (New England Biolabs P8107) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... 2) 7 µL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per slide was added for deglycosylation and incubated overnight at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... 7 μL of NEBNext Ultra II End Prep Enzyme buffer (NEB) and 3 μL of NEBNext Ultra II End Prep Enzyme Mix (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... and 15 uL of H2O) (New England Biolabs) for 1 hour at 37°C followed by DNase I (10 µL DNase I ...
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bioRxiv - Genomics 2024Quote: ... and 15 U Klenow exo (New England Biolabs), for 30 min at 37°C before inactivation for 20 min at 65°C ...
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bioRxiv - Genomics 2024Quote: ... 15 U T4 polynucleotide kinase (New England Biolabs), 5 U Klenow DNA polymerase (Roche) ...
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bioRxiv - Genomics 2023Quote: ... 15 μL 2x Q5U Master Mix (NEB M0597S), 0.4 μL 100 μM Nextera P5 index primer and 0.4 μL 100 μM Nextera P7 index primer ...
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bioRxiv - Cell Biology 2024Quote: ... at 15 minutes before nuclear envelope breakdown (NEB) no NSC had begun the rounding up process that marks mitotic entry ...
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bioRxiv - Biophysics 2020Quote: ... and loaded denatured (70 °C, 10 minutes) samples alongside an RNA ladder (2-4 μL, ssRNA ladder, N0362S, NEB). The resulting gels were stained with ethidium bromide for 30 minutes (0.5 μg/mL ddH20 ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were collected by centrifugation at 800g for 10 minutes at 4°C and resuspended in 1.2 X of NEB buffer 2.1 (New England Biolabs, B7202). 1 x 107 nuclei were then solubilized with 0.3% SDS for one hour at 37°C followed by adding 1.8% of TritonX-100 and incubating one hour at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...