Labshake search
Citations for New England Biolabs :
51 - 100 of 10000+ citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and real-time PCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on an ABI 7500 Fast RT-PCR system (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... We performed qPCR on a StepOneTM Real-Time PCR System with the Luna® Universal qPCR Master Mix (NEB). Gene specific primers are outlined in Extended Data Table S4.
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bioRxiv - Systems Biology 2022Quote: ... PCRs were performed using the Luna® Universal One Step RT-qPCR Kit (NEB) in a ThermoFisher Quantstudio 3 instrument ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was amplified by RT-PCR (Phusion High Fidelity Polymerase kit, New England Biolabs) using primers listed in Methods S3.
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bioRxiv - Microbiology 2024Quote: ... the NEB OneTaq® One-Step RT-PCR Kit (New England Biolabs, Product # E5315S) was used to target the haemagglutinin gene ...
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bioRxiv - Immunology 2022Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Immunology 2022Quote: ... integrated HIV DNA and unspliced HIV RNA was performed by modified nested real-time PCR assay using Taq DNA polymerase (BioLabs) in the first PCR and TaqMax Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplifications were performed in an Applied Biosystems 7500 Fast Real-Time PCR System using the Luna Universal Probe Mix (NEB). We evaluated the performance of the qPCR primers and probes using a DNA concentration gradient spanning 0.1-100.0 ng/reaction in single gene reactions as well as in a multiplex reaction ...
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bioRxiv - Microbiology 2020Quote: The quantification of DWV genome copies was performed by SYBR-Green Real-Time Quantitative PCR (qPCR) using Luna Universal qPCR master mix (New England Biolabs), 0.25 μM forward and reverse DWV_qPCR primers and 2 μl of cDNA ...
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bioRxiv - Microbiology 2022Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (NEB) with the primers listed in (Table S3) ...
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bioRxiv - Immunology 2024Quote: ... Two microliters were subsequently used in real-time quantitative PCR reactions containing SYBR-green based Luna universal dye qPCR mix (New England Biolabs) and gene specific PCR primers ...
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bioRxiv - Microbiology 2023Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) with the primers listed in Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) and the primers listed in Suppl ...
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bioRxiv - Neuroscience 2021Quote: ... Genes of interest were amplified with the One Taq One-Step RT-PCR kit (NEB) using 1 μg of RNA ...
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bioRxiv - Plant Biology 2021Quote: ... and reverse-transcribed following manufacturer’s recommendations (The ProtoScript Taq RT-PCR Kit, New England Biolabs). Relative mRNA ...
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bioRxiv - Immunology 2021Quote: ... and 60 ng were used for the One Taq RT PCR Kit (New England Biolabs) according to the instructions provided by the manufacturer for mRNA quantification and reverse transcription (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... and first strand cDNA was synthesized with One-Taq RT-PCR kit (New England Biolabs), according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... followed by cDNA synthesis using ProtoScript® Taq RT-PCR Kit [New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR with OneTaq (New England Biolabs #M0480S) was performed on cDNA preparations ...
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bioRxiv - Systems Biology 2019Quote: ... Both real-time and first-step PCRs were done similarly to the manufacturer’s protocol for Phusion polymerase (New England BioLabs, Ipswich, MA). For qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed to quantify the expression level of mRNAs by Luna® Universal qPCR Master Mix (NEB, M3003E) and LightCycler ®480 Real-Time PCR System (Roche) ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analysis was carried out using the Luna Universal One-Step RT-qPCR kit (NEB) using 100 ng RNA as input according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: Conventional RT-PCR was performed using Phusion® High-Fidelity DNA Polymerase kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... before cDNA was synthesized with the AMV LongAmp® Taq RT-PCR kit (New England Biolabs). A 500bp fragment covering the second and third exons common to all polymorphic MHC class I genes as amplified from each cDNA using NEB Go Taq polymerase and ovine MHC class I generic primers 416 and Cr as described in36 ...
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bioRxiv - Bioengineering 2024Quote: ... and cDNA libraries were generated by RT-PCR using the ProtoScript II cDNA Synthesis Kit (BioLabs) according to the manufacturers’ protocols ...
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bioRxiv - Microbiology 2022Quote: ... LunaScript RT SuperMix Kit (NEB) was used to generate cDNA using 1μg RNA ...
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bioRxiv - Immunology 2021Quote: ... directly into One Step RT-PCR reaction mix (NEB) loaded in MicroAmp Optical 96-well reaction plates (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB) using approximately 100-200 ng of total RNA per sample as input ...
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bioRxiv - Molecular Biology 2023Quote: ... All qRT-PCR reactions were performed with the Luna Universal One-Step RT-qPCR kit (E3005 NEB) according to manufacturer protocol with a few modifications ...
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bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
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bioRxiv - Cancer Biology 2022Quote: ... Using LunaScript RT Supermix kit (BioLabs), cDNA was prepared in a 20 μL reaction according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted RNA from light and heavy fractions were subjected to qRT-PCR using Luna RT-qPCR kit (NEB).
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis and qRT-PCR were performed simultaneously using the Luna Universal One-Step RT-qPCR kit (NEB) with the Quantstudio 7 Flex (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: PCR was carried out using NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) or Taqman Fast Universal PCR master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... The annealing temperatures for RT-PCR were calculated by NEB Tm Calculator (https://tmcalculator.neb.com/) ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR was carried out using Q5 DNA polymerase (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 viral RNA detection and quantification was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs, Whitby, ON, Canada) on the Rotor-gene Q platform (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... The RT-qPCRs with Luna Universal One-Step RT-qPCR kit (NEB, USA) were performed according to the protocol of the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR kit (NEB) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA and quantitative PCR was performed using the Luna Universal One-step RT-qPCR Kit (NEB) using 1 µg of RNA per reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Physiology 2022Quote: ... EnGen Mutation Detection Kit (New England BioLabs) was used for amplification of target DNA ...
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bioRxiv - Microbiology 2023Quote: ... LunaScript RT SuperMix Kit (New England Biolabs) was used to generate cDNA using 1µg RNA ...
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bioRxiv - Microbiology 2020Quote: ... The amplified 1,297 RT-PCR products were digested with MluI (NEB). Amplified DNA products ...